Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_707 and Pf1N1B4_708 are separated by 96 nucleotides Pf1N1B4_708 and Pf1N1B4_709 are separated by 96 nucleotides Pf1N1B4_709 and Pf1N1B4_710 are separated by 6 nucleotides
Pf1N1B4_707 - Nucleoside-diphosphate-sugar epimerases, at 806,937 to 807,929
_707
Pf1N1B4_708 - Chromosome initiation inhibitor, at 808,026 to 808,925
_708
Pf1N1B4_709 - FIG00953860: hypothetical protein, at 809,022 to 809,423
_709
Pf1N1B4_710 - Transporter, LysE family, at 809,430 to 810,032
_710
Position (kb)
808
809 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.208 kb on + strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.209 kb on - strand, within Pf1N1B4_707 at 807.256 kb on + strand, within Pf1N1B4_707 at 807.256 kb on + strand, within Pf1N1B4_707 at 807.257 kb on - strand, within Pf1N1B4_707 at 807.502 kb on + strand, within Pf1N1B4_707 at 807.503 kb on - strand, within Pf1N1B4_707 at 807.503 kb on - strand, within Pf1N1B4_707 at 807.503 kb on - strand at 807.583 kb on + strand, within Pf1N1B4_707 at 807.584 kb on - strand, within Pf1N1B4_707 at 807.584 kb on - strand, within Pf1N1B4_707 at 807.941 kb on - strand at 807.941 kb on - strand at 807.941 kb on - strand at 807.952 kb on + strand at 807.953 kb on - strand at 807.967 kb on - strand at 807.981 kb on - strand at 808.148 kb on + strand at 808.148 kb on + strand, within Pf1N1B4_708 at 808.148 kb on + strand, within Pf1N1B4_708 at 808.148 kb on + strand, within Pf1N1B4_708 at 808.149 kb on - strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.269 kb on + strand, within Pf1N1B4_708 at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand at 808.270 kb on - strand, within Pf1N1B4_708 at 808.270 kb on - strand, within Pf1N1B4_708 at 808.324 kb on - strand, within Pf1N1B4_708 at 808.549 kb on - strand, within Pf1N1B4_708 at 808.908 kb on + strand at 808.908 kb on + strand at 808.909 kb on - strand at 808.909 kb on - strand at 808.916 kb on - strand at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.088 kb on + strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.089 kb on - strand, within Pf1N1B4_709 at 809.220 kb on + strand, within Pf1N1B4_709 at 809.221 kb on - strand, within Pf1N1B4_709 at 809.221 kb on - strand at 809.221 kb on - strand, within Pf1N1B4_709 at 809.221 kb on - strand, within Pf1N1B4_709 at 809.341 kb on - strand, within Pf1N1B4_709 at 809.341 kb on - strand at 809.393 kb on - strand at 809.421 kb on + strand at 809.429 kb on + strand at 809.429 kb on + strand at 809.430 kb on - strand at 809.430 kb on - strand at 809.430 kb on - strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.442 kb on + strand at 809.669 kb on - strand, within Pf1N1B4_710 at 809.669 kb on - strand at 809.744 kb on - strand at 809.806 kb on - strand at 809.865 kb on + strand, within Pf1N1B4_710 at 809.865 kb on + strand, within Pf1N1B4_710 at 809.866 kb on - strand, within Pf1N1B4_710 at 809.866 kb on - strand, within Pf1N1B4_710 at 809.893 kb on + strand, within Pf1N1B4_710 at 809.893 kb on + strand, within Pf1N1B4_710 at 809.912 kb on - strand, within Pf1N1B4_710
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 807,208 + Pf1N1B4_707 0.27 -0.6 807,208 + Pf1N1B4_707 0.27 +0.6 807,208 + Pf1N1B4_707 0.27 -0.0 807,208 + +1.0 807,208 + Pf1N1B4_707 0.27 -0.6 807,208 + Pf1N1B4_707 0.27 +2.6 807,208 + Pf1N1B4_707 0.27 -1.6 807,209 - Pf1N1B4_707 0.27 -0.0 807,209 - Pf1N1B4_707 0.27 +0.8 807,209 - Pf1N1B4_707 0.27 -0.0 807,209 - Pf1N1B4_707 0.27 +0.6 807,209 - Pf1N1B4_707 0.27 +1.0 807,209 - Pf1N1B4_707 0.27 +0.1 807,209 - Pf1N1B4_707 0.27 -0.7 807,209 - Pf1N1B4_707 0.27 -0.7 807,256 + Pf1N1B4_707 0.32 -1.0 807,256 + Pf1N1B4_707 0.32 +1.0 807,257 - Pf1N1B4_707 0.32 -1.0 807,502 + Pf1N1B4_707 0.57 +0.3 807,503 - Pf1N1B4_707 0.57 -1.0 807,503 - Pf1N1B4_707 0.57 -2.0 807,503 - -0.0 807,583 + Pf1N1B4_707 0.65 -1.0 807,584 - Pf1N1B4_707 0.65 -0.8 807,584 - Pf1N1B4_707 0.65 -1.6 807,941 - +0.4 807,941 - +0.6 807,941 - -1.8 807,952 + -0.5 807,953 - -0.7 807,967 - +0.6 807,981 - -0.0 808,148 + -0.0 808,148 + Pf1N1B4_708 0.14 +1.0 808,148 + Pf1N1B4_708 0.14 -0.0 808,148 + Pf1N1B4_708 0.14 -2.8 808,149 - Pf1N1B4_708 0.14 -1.8 808,269 + Pf1N1B4_708 0.27 -1.0 808,269 + Pf1N1B4_708 0.27 -2.3 808,269 + -1.0 808,269 + Pf1N1B4_708 0.27 -0.3 808,269 + Pf1N1B4_708 0.27 -1.0 808,269 + Pf1N1B4_708 0.27 +1.8 808,269 + Pf1N1B4_708 0.27 +0.6 808,270 - Pf1N1B4_708 0.27 -2.3 808,270 - +0.6 808,270 - Pf1N1B4_708 0.27 -1.8 808,270 - -1.0 808,270 - Pf1N1B4_708 0.27 -0.7 808,270 - Pf1N1B4_708 0.27 -2.0 808,324 - Pf1N1B4_708 0.33 -1.4 808,549 - Pf1N1B4_708 0.58 -2.6 808,908 + -2.3 808,908 + -2.3 808,909 - -1.0 808,909 - -0.6 808,916 - -2.6 809,088 + Pf1N1B4_709 0.16 +1.2 809,088 + Pf1N1B4_709 0.16 -0.6 809,088 + Pf1N1B4_709 0.16 -0.6 809,088 + Pf1N1B4_709 0.16 -1.0 809,088 + Pf1N1B4_709 0.16 +0.7 809,089 - Pf1N1B4_709 0.17 -1.0 809,089 - Pf1N1B4_709 0.17 -1.6 809,089 - Pf1N1B4_709 0.17 -0.4 809,220 + Pf1N1B4_709 0.49 -0.7 809,221 - Pf1N1B4_709 0.50 -1.6 809,221 - -1.0 809,221 - Pf1N1B4_709 0.50 -0.2 809,221 - Pf1N1B4_709 0.50 +1.0 809,341 - Pf1N1B4_709 0.79 -0.0 809,341 - -1.0 809,393 - +1.3 809,421 + +2.0 809,429 + -0.6 809,429 + -0.7 809,430 - -0.7 809,430 - -1.0 809,430 - -1.6 809,442 + -0.6 809,442 + -2.0 809,442 + +1.2 809,442 + -2.0 809,442 + -0.0 809,442 + +0.7 809,669 - Pf1N1B4_710 0.40 +1.6 809,669 - +1.0 809,744 - +1.6 809,806 - +1.0 809,865 + Pf1N1B4_710 0.72 +0.0 809,865 + Pf1N1B4_710 0.72 +0.2 809,866 - Pf1N1B4_710 0.72 +0.0 809,866 - Pf1N1B4_710 0.72 -0.5 809,893 + Pf1N1B4_710 0.77 -1.0 809,893 + Pf1N1B4_710 0.77 -0.9 809,912 - Pf1N1B4_710 0.80 +0.0
Or see this region's nucleotide sequence