Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5906 and Pf1N1B4_5907 are separated by 63 nucleotides Pf1N1B4_5907 and Pf1N1B4_5908 are separated by 89 nucleotides Pf1N1B4_5908 and Pf1N1B4_5909 are separated by 70 nucleotides
Pf1N1B4_5906 - Esterase/lipase, at 348,829 to 349,845
_5906
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Pf1N1B4_5908 - Nicotinamidase family protein YcaC, at 351,426 to 352,052
_5908
Pf1N1B4_5909 - Exoenzymes regulatory protein AepA precursor, at 352,123 to 353,961
_5909
Position (kb) 349
350
351
352 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 349.234 kb on + strand, within Pf1N1B4_5906 at 349.235 kb on - strand, within Pf1N1B4_5906 at 349.292 kb on - strand, within Pf1N1B4_5906 at 349.553 kb on - strand, within Pf1N1B4_5906 at 349.688 kb on - strand, within Pf1N1B4_5906 at 349.736 kb on - strand, within Pf1N1B4_5906 at 349.805 kb on + strand at 349.844 kb on - strand at 349.844 kb on - strand at 349.844 kb on - strand at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.281 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.728 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand, within Pf1N1B4_5907 at 350.956 kb on + strand at 351.166 kb on + strand, within Pf1N1B4_5907 at 351.167 kb on - strand, within Pf1N1B4_5907 at 351.367 kb on + strand at 351.367 kb on + strand at 351.367 kb on + strand at 351.391 kb on + strand at 351.391 kb on + strand at 351.469 kb on + strand at 351.709 kb on - strand, within Pf1N1B4_5908 at 352.005 kb on + strand at 352.037 kb on - strand at 352.037 kb on - strand at 352.079 kb on - strand at 352.079 kb on - strand at 352.124 kb on + strand at 352.124 kb on + strand at 352.124 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 349,234 + Pf1N1B4_5906 0.40 -3.4 349,235 - Pf1N1B4_5906 0.40 -2.4 349,292 - Pf1N1B4_5906 0.46 -1.4 349,553 - Pf1N1B4_5906 0.71 -1.4 349,688 - Pf1N1B4_5906 0.84 -0.8 349,736 - Pf1N1B4_5906 0.89 -2.3 349,805 + -0.4 349,844 - -1.0 349,844 - +0.6 349,844 - +0.6 350,138 + Pf1N1B4_5907 0.16 -0.6 350,138 + Pf1N1B4_5907 0.16 -0.9 350,204 + Pf1N1B4_5907 0.21 -2.4 350,204 + Pf1N1B4_5907 0.21 -0.1 350,204 + Pf1N1B4_5907 0.21 +1.1 350,205 - Pf1N1B4_5907 0.21 -0.8 350,205 - Pf1N1B4_5907 0.21 -1.0 350,205 - Pf1N1B4_5907 0.21 +2.7 350,281 + Pf1N1B4_5907 0.26 -1.4 350,361 + Pf1N1B4_5907 0.32 +1.6 350,361 + Pf1N1B4_5907 0.32 +0.6 350,728 + Pf1N1B4_5907 0.57 +0.6 350,956 + Pf1N1B4_5907 0.73 -1.2 350,956 + -0.4 351,166 + Pf1N1B4_5907 0.88 +0.8 351,167 - Pf1N1B4_5907 0.88 -0.4 351,367 + -0.9 351,367 + -0.0 351,367 + -0.1 351,391 + -0.4 351,391 + -0.4 351,469 + +0.2 351,709 - Pf1N1B4_5908 0.45 -1.8 352,005 + -2.8 352,037 - -1.4 352,037 - -1.8 352,079 - +0.1 352,079 - -0.8 352,124 + +0.6 352,124 + -3.0 352,124 + +1.6
Or see this region's nucleotide sequence