Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5905 and Pf1N1B4_5906 are separated by 162 nucleotides Pf1N1B4_5906 and Pf1N1B4_5907 are separated by 63 nucleotides
Pf1N1B4_5905 - Transcriptional regulator, LysR family, at 347,761 to 348,666
_5905
Pf1N1B4_5906 - Esterase/lipase, at 348,829 to 349,845
_5906
Pf1N1B4_5907 - cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases, at 349,909 to 351,336
_5907
Position (kb) 348
349
350 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 347.957 kb on + strand, within Pf1N1B4_5905 at 348.115 kb on + strand, within Pf1N1B4_5905 at 348.116 kb on - strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.251 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand at 348.474 kb on - strand, within Pf1N1B4_5905 at 348.589 kb on + strand at 348.589 kb on + strand at 348.590 kb on - strand at 348.642 kb on + strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.648 kb on + strand at 348.812 kb on - strand at 348.813 kb on + strand at 348.814 kb on - strand at 348.814 kb on - strand at 348.814 kb on - strand at 349.234 kb on + strand, within Pf1N1B4_5906 at 349.235 kb on - strand, within Pf1N1B4_5906 at 349.292 kb on - strand, within Pf1N1B4_5906 at 349.553 kb on - strand, within Pf1N1B4_5906 at 349.688 kb on - strand, within Pf1N1B4_5906 at 349.736 kb on - strand, within Pf1N1B4_5906 at 349.805 kb on + strand at 349.844 kb on - strand at 349.844 kb on - strand at 349.844 kb on - strand at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.138 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.204 kb on + strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.205 kb on - strand, within Pf1N1B4_5907 at 350.281 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.361 kb on + strand, within Pf1N1B4_5907 at 350.728 kb on + strand, within Pf1N1B4_5907
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 347,957 + Pf1N1B4_5905 0.22 -0.1 348,115 + Pf1N1B4_5905 0.39 -1.2 348,116 - Pf1N1B4_5905 0.39 -0.4 348,155 + Pf1N1B4_5905 0.43 +0.1 348,155 + Pf1N1B4_5905 0.43 -0.1 348,155 + Pf1N1B4_5905 0.43 -1.4 348,251 + Pf1N1B4_5905 0.54 +0.7 348,473 + Pf1N1B4_5905 0.79 +0.8 348,473 + -2.8 348,474 - Pf1N1B4_5905 0.79 -1.0 348,589 + -1.4 348,589 + +1.1 348,590 - +0.4 348,642 + -1.4 348,643 - -1.4 348,643 - +0.6 348,643 - +0.6 348,648 + +0.9 348,812 - +0.1 348,813 + -1.4 348,814 - -0.1 348,814 - +0.6 348,814 - +0.4 349,234 + Pf1N1B4_5906 0.40 -3.4 349,235 - Pf1N1B4_5906 0.40 -2.4 349,292 - Pf1N1B4_5906 0.46 -1.4 349,553 - Pf1N1B4_5906 0.71 -1.4 349,688 - Pf1N1B4_5906 0.84 -0.8 349,736 - Pf1N1B4_5906 0.89 -2.3 349,805 + -0.4 349,844 - -1.0 349,844 - +0.6 349,844 - +0.6 350,138 + Pf1N1B4_5907 0.16 -0.6 350,138 + Pf1N1B4_5907 0.16 -0.9 350,204 + Pf1N1B4_5907 0.21 -2.4 350,204 + Pf1N1B4_5907 0.21 -0.1 350,204 + Pf1N1B4_5907 0.21 +1.1 350,205 - Pf1N1B4_5907 0.21 -0.8 350,205 - Pf1N1B4_5907 0.21 -1.0 350,205 - Pf1N1B4_5907 0.21 +2.7 350,281 + Pf1N1B4_5907 0.26 -1.4 350,361 + Pf1N1B4_5907 0.32 +1.6 350,361 + Pf1N1B4_5907 0.32 +0.6 350,728 + Pf1N1B4_5907 0.57 +0.6
Or see this region's nucleotide sequence