Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5901 and Pf1N1B4_5902 are separated by 305 nucleotides Pf1N1B4_5902 and Pf1N1B4_5903 are separated by 2 nucleotides Pf1N1B4_5903 and Pf1N1B4_5904 overlap by 12 nucleotides Pf1N1B4_5904 and Pf1N1B4_5905 are separated by 24 nucleotides
Pf1N1B4_5901 - hypothetical protein, at 345,393 to 346,082
_5901
Pf1N1B4_5902 - FIG022160: hypothetical toxin, at 346,388 to 346,528
_5902
Pf1N1B4_5903 - FIG045511: hypothetical antitoxin (to FIG022160: hypothetical toxin), at 346,531 to 346,839
_5903
Pf1N1B4_5904 - FIG00955320: hypothetical protein, at 346,828 to 347,736
_5904
Pf1N1B4_5905 - Transcriptional regulator, LysR family, at 347,761 to 348,666
_5905
Position (kb) 346
347
348 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 345.846 kb on - strand, within Pf1N1B4_5901 at 345.943 kb on - strand, within Pf1N1B4_5901 at 345.972 kb on + strand, within Pf1N1B4_5901 at 345.973 kb on - strand, within Pf1N1B4_5901 at 346.015 kb on - strand at 346.181 kb on - strand at 346.181 kb on - strand at 346.223 kb on + strand at 346.224 kb on - strand at 346.224 kb on - strand at 346.224 kb on - strand at 346.242 kb on - strand at 346.247 kb on - strand at 346.432 kb on - strand, within Pf1N1B4_5902 at 346.527 kb on - strand at 346.598 kb on - strand at 346.767 kb on - strand, within Pf1N1B4_5903 at 346.856 kb on - strand at 346.886 kb on + strand at 346.928 kb on + strand, within Pf1N1B4_5904 at 346.929 kb on - strand, within Pf1N1B4_5904 at 347.034 kb on - strand, within Pf1N1B4_5904 at 347.180 kb on + strand, within Pf1N1B4_5904 at 347.957 kb on + strand, within Pf1N1B4_5905 at 348.115 kb on + strand, within Pf1N1B4_5905 at 348.116 kb on - strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.155 kb on + strand, within Pf1N1B4_5905 at 348.251 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand, within Pf1N1B4_5905 at 348.473 kb on + strand at 348.474 kb on - strand, within Pf1N1B4_5905 at 348.589 kb on + strand at 348.589 kb on + strand at 348.590 kb on - strand at 348.642 kb on + strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.643 kb on - strand at 348.648 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 345,846 - Pf1N1B4_5901 0.66 -0.0 345,943 - Pf1N1B4_5901 0.80 -0.4 345,972 + Pf1N1B4_5901 0.84 +1.7 345,973 - Pf1N1B4_5901 0.84 +0.1 346,015 - -1.0 346,181 - +0.1 346,181 - -0.4 346,223 + +0.4 346,224 - +0.0 346,224 - +1.4 346,224 - -2.3 346,242 - -1.3 346,247 - +0.1 346,432 - Pf1N1B4_5902 0.31 -0.0 346,527 - -0.4 346,598 - -0.4 346,767 - Pf1N1B4_5903 0.76 +0.3 346,856 - +0.0 346,886 + -0.0 346,928 + Pf1N1B4_5904 0.11 -0.8 346,929 - Pf1N1B4_5904 0.11 -0.4 347,034 - Pf1N1B4_5904 0.23 -0.8 347,180 + Pf1N1B4_5904 0.39 +0.9 347,957 + Pf1N1B4_5905 0.22 -0.1 348,115 + Pf1N1B4_5905 0.39 -1.2 348,116 - Pf1N1B4_5905 0.39 -0.4 348,155 + Pf1N1B4_5905 0.43 +0.1 348,155 + Pf1N1B4_5905 0.43 -0.1 348,155 + Pf1N1B4_5905 0.43 -1.4 348,251 + Pf1N1B4_5905 0.54 +0.7 348,473 + Pf1N1B4_5905 0.79 +0.8 348,473 + -2.8 348,474 - Pf1N1B4_5905 0.79 -1.0 348,589 + -1.4 348,589 + +1.1 348,590 - +0.4 348,642 + -1.4 348,643 - -1.4 348,643 - +0.6 348,643 - +0.6 348,648 + +0.9
Or see this region's nucleotide sequence