Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5896 and Pf1N1B4_5897 overlap by 4 nucleotides Pf1N1B4_5897 and Pf1N1B4_5898 are separated by 173 nucleotides Pf1N1B4_5898 and Pf1N1B4_5899 are separated by 4 nucleotides
Pf1N1B4_5896 - Polysaccharide biosynthesis protein, at 339,330 to 340,634
_5896
Pf1N1B4_5897 - FIG00963512: hypothetical protein, at 340,631 to 342,550
_5897
Pf1N1B4_5898 - hypothetical protein, at 342,724 to 343,332
_5898
Pf1N1B4_5899 - FIG00954746: hypothetical protein, at 343,337 to 344,551
_5899
Position (kb) 340
341
342
343 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 340.202 kb on - strand, within Pf1N1B4_5896 at 340.291 kb on - strand at 340.536 kb on + strand at 340.536 kb on + strand at 340.536 kb on + strand at 340.537 kb on - strand at 340.537 kb on - strand at 340.537 kb on - strand at 340.705 kb on + strand at 340.757 kb on - strand at 340.929 kb on - strand, within Pf1N1B4_5897 at 341.033 kb on + strand, within Pf1N1B4_5897 at 341.033 kb on + strand, within Pf1N1B4_5897 at 341.034 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.382 kb on - strand, within Pf1N1B4_5897 at 341.391 kb on - strand, within Pf1N1B4_5897 at 341.456 kb on + strand, within Pf1N1B4_5897 at 341.517 kb on - strand, within Pf1N1B4_5897 at 341.664 kb on - strand, within Pf1N1B4_5897 at 341.696 kb on + strand, within Pf1N1B4_5897 at 341.697 kb on - strand, within Pf1N1B4_5897 at 341.705 kb on + strand, within Pf1N1B4_5897 at 341.748 kb on + strand, within Pf1N1B4_5897 at 342.358 kb on - strand, within Pf1N1B4_5897 at 342.368 kb on - strand at 342.437 kb on + strand at 342.438 kb on - strand at 342.643 kb on - strand at 342.643 kb on - strand at 342.721 kb on + strand at 342.721 kb on + strand at 342.905 kb on - strand, within Pf1N1B4_5898 at 342.919 kb on + strand, within Pf1N1B4_5898 at 342.920 kb on - strand, within Pf1N1B4_5898 at 342.991 kb on + strand at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.158 kb on + strand, within Pf1N1B4_5898 at 343.159 kb on - strand, within Pf1N1B4_5898 at 343.161 kb on - strand, within Pf1N1B4_5898 at 343.430 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 340,202 - Pf1N1B4_5896 0.67 -0.4 340,291 - +0.6 340,536 + -0.7 340,536 + -0.4 340,536 + -1.2 340,537 - +0.3 340,537 - -1.1 340,537 - -0.8 340,705 + +0.3 340,757 - -0.4 340,929 - Pf1N1B4_5897 0.16 -0.6 341,033 + Pf1N1B4_5897 0.21 -1.4 341,033 + Pf1N1B4_5897 0.21 +2.1 341,034 - Pf1N1B4_5897 0.21 -1.2 341,382 - Pf1N1B4_5897 0.39 -0.0 341,382 - Pf1N1B4_5897 0.39 -2.8 341,382 - Pf1N1B4_5897 0.39 -2.0 341,391 - Pf1N1B4_5897 0.40 -1.4 341,456 + Pf1N1B4_5897 0.43 -2.0 341,517 - Pf1N1B4_5897 0.46 -0.0 341,664 - Pf1N1B4_5897 0.54 +0.3 341,696 + Pf1N1B4_5897 0.55 -1.0 341,697 - Pf1N1B4_5897 0.56 -2.4 341,705 + Pf1N1B4_5897 0.56 -0.4 341,748 + Pf1N1B4_5897 0.58 -0.4 342,358 - Pf1N1B4_5897 0.90 -1.4 342,368 - -0.4 342,437 + -1.0 342,438 - +0.1 342,643 - -3.0 342,643 - +0.9 342,721 + -1.5 342,721 + +2.4 342,905 - Pf1N1B4_5898 0.30 -2.4 342,919 + Pf1N1B4_5898 0.32 -2.0 342,920 - Pf1N1B4_5898 0.32 -0.8 342,991 + +0.1 343,158 + Pf1N1B4_5898 0.71 -1.2 343,158 + Pf1N1B4_5898 0.71 +1.1 343,159 - Pf1N1B4_5898 0.71 -0.9 343,161 - Pf1N1B4_5898 0.72 -2.0 343,430 - -0.4
Or see this region's nucleotide sequence