Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5813 and Pf1N1B4_5814 are separated by 60 nucleotides Pf1N1B4_5814 and Pf1N1B4_5815 are separated by 15 nucleotides Pf1N1B4_5815 and Pf1N1B4_5816 are separated by 150 nucleotides Pf1N1B4_5816 and Pf1N1B4_5817 are separated by 227 nucleotides
Pf1N1B4_5813 - 4-hydroxy-tetrahydrodipicolinate synthase (EC 4.3.3.7), at 245,881 to 246,756
_5813
Pf1N1B4_5814 - putative membrane protein, at 246,817 to 247,203
_5814
Pf1N1B4_5815 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 247,219 to 248,796
_5815
Pf1N1B4_5816 - FIG00957304: hypothetical protein, at 248,947 to 249,474
_5816
Pf1N1B4_5817 - Decarboxylase family protein, at 249,702 to 251,075
_5817
Position (kb) 247
248
249 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 246.458 kb on + strand, within Pf1N1B4_5813 at 246.582 kb on - strand, within Pf1N1B4_5813 at 246.755 kb on - strand at 246.797 kb on + strand at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.057 kb on + strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.058 kb on - strand, within Pf1N1B4_5814 at 247.147 kb on + strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.148 kb on - strand, within Pf1N1B4_5814 at 247.265 kb on + strand at 247.265 kb on + strand at 247.266 kb on - strand at 247.734 kb on + strand, within Pf1N1B4_5815 at 247.785 kb on + strand, within Pf1N1B4_5815 at 247.788 kb on + strand, within Pf1N1B4_5815 at 247.789 kb on - strand, within Pf1N1B4_5815 at 248.186 kb on + strand, within Pf1N1B4_5815 at 248.186 kb on + strand at 248.187 kb on - strand, within Pf1N1B4_5815 at 248.327 kb on + strand, within Pf1N1B4_5815 at 248.327 kb on + strand at 248.336 kb on + strand, within Pf1N1B4_5815 at 248.815 kb on + strand at 248.883 kb on - strand at 248.900 kb on - strand at 248.949 kb on - strand at 249.376 kb on + strand at 249.377 kb on - strand, within Pf1N1B4_5816 at 249.441 kb on - strand at 249.502 kb on - strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.555 kb on + strand at 249.556 kb on - strand at 249.556 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.560 kb on - strand at 249.611 kb on - strand at 249.642 kb on + strand at 249.649 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.718 kb on + strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.719 kb on - strand at 249.741 kb on - strand at 249.776 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 246,458 + Pf1N1B4_5813 0.66 -1.5 246,582 - Pf1N1B4_5813 0.80 -1.5 246,755 - -1.5 246,797 + -1.9 247,057 + Pf1N1B4_5814 0.62 +0.2 247,057 + Pf1N1B4_5814 0.62 +1.1 247,058 - Pf1N1B4_5814 0.62 -1.0 247,058 - Pf1N1B4_5814 0.62 -0.5 247,147 + Pf1N1B4_5814 0.85 +0.5 247,148 - Pf1N1B4_5814 0.86 +0.9 247,148 - Pf1N1B4_5814 0.86 -0.1 247,265 + +0.2 247,265 + +1.1 247,266 - -0.9 247,734 + Pf1N1B4_5815 0.33 -0.5 247,785 + Pf1N1B4_5815 0.36 -0.5 247,788 + Pf1N1B4_5815 0.36 -1.7 247,789 - Pf1N1B4_5815 0.36 -1.5 248,186 + Pf1N1B4_5815 0.61 -0.5 248,186 + -1.5 248,187 - Pf1N1B4_5815 0.61 +0.1 248,327 + Pf1N1B4_5815 0.70 -2.8 248,327 + -0.5 248,336 + Pf1N1B4_5815 0.71 -0.7 248,815 + +0.5 248,883 - +1.5 248,900 - +1.4 248,949 - -0.5 249,376 + -1.4 249,377 - Pf1N1B4_5816 0.81 +0.8 249,441 - +1.0 249,502 - -2.0 249,555 + +1.1 249,555 + -1.4 249,555 + -2.4 249,555 + -2.6 249,555 + -1.4 249,555 + -0.4 249,556 - +0.1 249,556 - -0.4 249,560 - -1.4 249,560 - +0.3 249,560 - -3.3 249,611 - +0.1 249,642 + -1.4 249,649 + -1.0 249,718 + -2.0 249,718 + +1.1 249,718 + -0.2 249,719 - -1.4 249,719 - -1.8 249,719 - -0.4 249,719 - +0.1 249,719 - +2.1 249,741 - +1.1 249,776 - -0.4
Or see this region's nucleotide sequence