Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides Pf1N1B4_5723 and Pf1N1B4_5724 are separated by 34 nucleotides Pf1N1B4_5724 and Pf1N1B4_5725 overlap by 16 nucleotides Pf1N1B4_5725 and Pf1N1B4_5726 are separated by 38 nucleotides
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Pf1N1B4_5724 - High-affinity iron permease, at 164,459 to 164,941
_5724
Pf1N1B4_5725 - High-affinity iron permease, at 164,926 to 165,300
_5725
Pf1N1B4_5726 - Ferredoxin, at 165,339 to 166,667
_5726
Position (kb) 164
165 Strain fitness (log2 ratio) -2
-1
0
1
2 at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.798 kb on + strand, within Pf1N1B4_5724 at 164.801 kb on + strand, within Pf1N1B4_5724 at 164.802 kb on - strand, within Pf1N1B4_5724 at 165.101 kb on - strand, within Pf1N1B4_5725 at 165.385 kb on + strand at 165.394 kb on - strand at 165.485 kb on - strand, within Pf1N1B4_5726 at 165.594 kb on + strand, within Pf1N1B4_5726 at 165.699 kb on + strand, within Pf1N1B4_5726
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 163,472 + +0.5 163,537 - -2.1 163,558 + +0.7 163,559 - -0.2 163,559 - +0.7 163,559 - -1.1 163,561 + -0.5 163,562 - -0.0 163,568 - -0.4 163,679 + Pf1N1B4_5721 0.76 +1.0 163,679 + Pf1N1B4_5721 0.76 -0.2 163,680 - Pf1N1B4_5721 0.77 +0.2 163,680 - Pf1N1B4_5721 0.77 +1.1 163,916 + Pf1N1B4_5722 0.54 -1.5 163,916 + Pf1N1B4_5722 0.54 -0.5 163,916 + Pf1N1B4_5722 0.54 +1.1 163,917 - Pf1N1B4_5722 0.54 -0.5 163,936 - Pf1N1B4_5722 0.59 -2.5 164,798 + Pf1N1B4_5724 0.70 +0.5 164,798 + Pf1N1B4_5724 0.70 -0.8 164,798 + Pf1N1B4_5724 0.70 -1.1 164,801 + Pf1N1B4_5724 0.71 -1.1 164,802 - Pf1N1B4_5724 0.71 -1.5 165,101 - Pf1N1B4_5725 0.47 +0.6 165,385 + -0.9 165,394 - +0.2 165,485 - Pf1N1B4_5726 0.11 -0.8 165,594 + Pf1N1B4_5726 0.19 +1.8 165,699 + Pf1N1B4_5726 0.27 +0.4
Or see this region's nucleotide sequence