Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5719 and Pf1N1B4_5720 are separated by 67 nucleotides Pf1N1B4_5720 and Pf1N1B4_5721 are separated by 205 nucleotides Pf1N1B4_5721 and Pf1N1B4_5722 overlap by 4 nucleotides Pf1N1B4_5722 and Pf1N1B4_5723 are separated by 0 nucleotides
Pf1N1B4_5719 - hypothetical protein, at 162,369 to 163,043
_5719
Pf1N1B4_5720 - hypothetical protein, at 163,111 to 163,356
_5720
Pf1N1B4_5721 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,562 to 163,714
_5721
Pf1N1B4_5722 - Periplasmic protein p19 involved in high-affinity Fe2+ transport, at 163,711 to 164,091
_5722
Pf1N1B4_5723 - putative exported protein, at 164,092 to 164,424
_5723
Position (kb)
163
164 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 162.131 kb on - strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.141 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.190 kb on + strand at 162.298 kb on + strand at 162.298 kb on + strand at 162.304 kb on + strand at 162.305 kb on - strand at 162.305 kb on - strand at 162.355 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.390 kb on + strand at 162.391 kb on - strand at 162.413 kb on + strand at 162.413 kb on + strand at 162.759 kb on + strand, within Pf1N1B4_5719 at 162.760 kb on - strand, within Pf1N1B4_5719 at 162.876 kb on - strand, within Pf1N1B4_5719 at 162.957 kb on + strand, within Pf1N1B4_5719 at 162.958 kb on - strand at 163.056 kb on + strand at 163.058 kb on + strand at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.174 kb on - strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.176 kb on + strand, within Pf1N1B4_5720 at 163.177 kb on - strand, within Pf1N1B4_5720 at 163.394 kb on - strand at 163.394 kb on - strand at 163.394 kb on - strand at 163.472 kb on + strand at 163.537 kb on - strand at 163.558 kb on + strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.559 kb on - strand at 163.561 kb on + strand at 163.562 kb on - strand at 163.568 kb on - strand at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.679 kb on + strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.680 kb on - strand, within Pf1N1B4_5721 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.916 kb on + strand, within Pf1N1B4_5722 at 163.917 kb on - strand, within Pf1N1B4_5722 at 163.936 kb on - strand, within Pf1N1B4_5722
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 162,131 - -0.3 162,141 + -0.5 162,141 + +0.1 162,141 + +0.9 162,190 + +0.1 162,190 + +2.1 162,190 + +0.0 162,298 + +0.0 162,298 + -1.5 162,304 + +0.8 162,305 - -0.2 162,305 - -0.7 162,355 + -1.5 162,390 + +0.7 162,390 + +0.5 162,390 + -1.5 162,391 - -0.5 162,413 + +0.3 162,413 + +0.5 162,759 + Pf1N1B4_5719 0.58 +0.0 162,760 - Pf1N1B4_5719 0.58 -0.5 162,876 - Pf1N1B4_5719 0.75 -0.2 162,957 + Pf1N1B4_5719 0.87 -0.8 162,958 - +0.5 163,056 + -0.5 163,058 + +0.1 163,174 - Pf1N1B4_5720 0.26 +0.1 163,174 - Pf1N1B4_5720 0.26 -2.3 163,176 + Pf1N1B4_5720 0.26 +0.1 163,176 + Pf1N1B4_5720 0.26 -2.1 163,177 - Pf1N1B4_5720 0.27 -0.5 163,394 - -0.1 163,394 - +0.7 163,394 - -0.5 163,472 + +0.5 163,537 - -2.1 163,558 + +0.7 163,559 - -0.2 163,559 - +0.7 163,559 - -1.1 163,561 + -0.5 163,562 - -0.0 163,568 - -0.4 163,679 + Pf1N1B4_5721 0.76 +1.0 163,679 + Pf1N1B4_5721 0.76 -0.2 163,680 - Pf1N1B4_5721 0.77 +0.2 163,680 - Pf1N1B4_5721 0.77 +1.1 163,916 + Pf1N1B4_5722 0.54 -1.5 163,916 + Pf1N1B4_5722 0.54 -0.5 163,916 + Pf1N1B4_5722 0.54 +1.1 163,917 - Pf1N1B4_5722 0.54 -0.5 163,936 - Pf1N1B4_5722 0.59 -2.5
Or see this region's nucleotide sequence