Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_5711

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_5709 and Pf1N1B4_5710 are separated by 22 nucleotidesPf1N1B4_5710 and Pf1N1B4_5711 are separated by 92 nucleotidesPf1N1B4_5711 and Pf1N1B4_5712 are separated by 336 nucleotides Pf1N1B4_5709 - 2,4-dienoyl-CoA reductase [NADPH] (EC 1.3.1.34), at 147,817 to 149,052 _5709 Pf1N1B4_5710 - Transcriptional regulator, TetR family, at 149,075 to 149,629 _5710 Pf1N1B4_5711 - Salicylate hydroxylase (EC 1.14.13.1), at 149,722 to 151,380 _5711 Pf1N1B4_5712 - Adenylate cyclase (EC 4.6.1.1) / Guanylate cyclase (EC 4.6.1.2), at 151,717 to 154,614 _5712 Position (kb) 149 150 151 152Strain fitness (log2 ratio) -2 -1 0 1at 149.026 kb on + strandat 149.121 kb on - strandat 149.175 kb on - strand, within Pf1N1B4_5710at 149.219 kb on + strand, within Pf1N1B4_5710at 149.219 kb on + strand, within Pf1N1B4_5710at 149.526 kb on + strand, within Pf1N1B4_5710at 149.527 kb on - strand, within Pf1N1B4_5710at 149.732 kb on - strandat 149.856 kb on + strandat 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.374 kb on + strand, within Pf1N1B4_5711at 150.385 kb on + strand, within Pf1N1B4_5711at 150.489 kb on + strandat 150.809 kb on + strand, within Pf1N1B4_5711at 150.809 kb on + strand, within Pf1N1B4_5711at 150.810 kb on - strandat 150.810 kb on - strand, within Pf1N1B4_5711at 151.014 kb on + strand, within Pf1N1B4_5711at 151.014 kb on + strand, within Pf1N1B4_5711at 151.256 kb on - strandat 151.506 kb on - strandat 151.526 kb on + strandat 151.526 kb on + strandat 151.527 kb on - strandat 151.685 kb on - strandat 151.799 kb on + strandat 151.799 kb on + strandat 151.853 kb on + strandat 152.171 kb on - strand, within Pf1N1B4_5712

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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149,026 + +0.5
149,121 - -1.5
149,175 - Pf1N1B4_5710 0.18 -0.2
149,219 + Pf1N1B4_5710 0.26 -1.1
149,219 + Pf1N1B4_5710 0.26 +0.5
149,526 + Pf1N1B4_5710 0.81 -0.2
149,527 - Pf1N1B4_5710 0.81 -0.1
149,732 - +0.1
149,856 + +1.1
150,374 + Pf1N1B4_5711 0.39 -2.5
150,374 + Pf1N1B4_5711 0.39 -1.5
150,374 + Pf1N1B4_5711 0.39 -0.8
150,374 + Pf1N1B4_5711 0.39 +1.7
150,385 + Pf1N1B4_5711 0.40 -2.5
150,489 + -2.5
150,809 + Pf1N1B4_5711 0.66 -0.5
150,809 + Pf1N1B4_5711 0.66 -2.4
150,810 - -2.5
150,810 - Pf1N1B4_5711 0.66 -1.1
151,014 + Pf1N1B4_5711 0.78 -1.5
151,014 + Pf1N1B4_5711 0.78 +1.4
151,256 - -0.1
151,506 - +1.4
151,526 + -0.5
151,526 + +0.5
151,527 - -1.8
151,685 - -1.5
151,799 + +1.5
151,799 + -2.3
151,853 + -1.7
152,171 - Pf1N1B4_5712 0.16 +0.5

Or see this region's nucleotide sequence