Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5700 and Pf1N1B4_5701 are separated by 169 nucleotides Pf1N1B4_5701 and Pf1N1B4_5702 overlap by 14 nucleotides Pf1N1B4_5702 and Pf1N1B4_5703 are separated by 42 nucleotides Pf1N1B4_5703 and Pf1N1B4_5704 are separated by 82 nucleotides
Pf1N1B4_5700 - Putative tRNA-m1A22 methylase, at 139,167 to 139,841
_5700
Pf1N1B4_5701 - FIG00955602: hypothetical protein, at 140,011 to 140,172
_5701
Pf1N1B4_5702 - hypothetical protein, at 140,159 to 140,320
_5702
Pf1N1B4_5703 - FIG00961921: hypothetical protein, at 140,363 to 141,073
_5703
Pf1N1B4_5704 - Long-chain fatty acid transport protein, at 141,156 to 142,475
_5704
Position (kb)
140
141 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 139.058 kb on + strand at 139.065 kb on - strand at 139.096 kb on - strand at 139.096 kb on - strand at 139.144 kb on + strand at 139.346 kb on - strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.363 kb on + strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.364 kb on - strand, within Pf1N1B4_5700 at 139.403 kb on - strand, within Pf1N1B4_5700 at 139.406 kb on - strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.679 kb on + strand, within Pf1N1B4_5700 at 139.812 kb on + strand at 139.812 kb on + strand at 139.812 kb on + strand at 139.813 kb on - strand at 139.813 kb on - strand at 139.876 kb on + strand at 139.877 kb on - strand at 140.028 kb on + strand, within Pf1N1B4_5701 at 140.076 kb on + strand, within Pf1N1B4_5701 at 140.077 kb on - strand, within Pf1N1B4_5701 at 140.077 kb on - strand at 140.077 kb on - strand, within Pf1N1B4_5701 at 140.085 kb on - strand at 140.088 kb on + strand, within Pf1N1B4_5701 at 140.089 kb on - strand, within Pf1N1B4_5701 at 140.095 kb on - strand, within Pf1N1B4_5701 at 140.095 kb on - strand, within Pf1N1B4_5701 at 140.141 kb on - strand, within Pf1N1B4_5701 at 140.143 kb on + strand, within Pf1N1B4_5701 at 140.143 kb on + strand, within Pf1N1B4_5701 at 140.172 kb on + strand at 140.172 kb on + strand at 140.328 kb on - strand at 140.339 kb on + strand at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.654 kb on + strand, within Pf1N1B4_5703 at 140.655 kb on - strand, within Pf1N1B4_5703 at 140.696 kb on + strand, within Pf1N1B4_5703 at 140.835 kb on - strand, within Pf1N1B4_5703 at 141.049 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 139,058 + +0.3 139,065 - -0.9 139,096 - +0.1 139,096 - +1.5 139,144 + +0.3 139,346 - Pf1N1B4_5700 0.27 -0.6 139,363 + Pf1N1B4_5700 0.29 +1.5 139,363 + Pf1N1B4_5700 0.29 -1.5 139,363 + Pf1N1B4_5700 0.29 +0.4 139,364 - Pf1N1B4_5700 0.29 +0.0 139,364 - Pf1N1B4_5700 0.29 -1.7 139,364 - Pf1N1B4_5700 0.29 +0.4 139,403 - Pf1N1B4_5700 0.35 +0.3 139,406 - Pf1N1B4_5700 0.35 +0.8 139,679 + Pf1N1B4_5700 0.76 -1.5 139,679 + Pf1N1B4_5700 0.76 -2.5 139,812 + -1.1 139,812 + +0.2 139,812 + -0.3 139,813 - -0.8 139,813 - +0.1 139,876 + -0.5 139,877 - -0.1 140,028 + Pf1N1B4_5701 0.10 -1.5 140,076 + Pf1N1B4_5701 0.40 -1.8 140,077 - Pf1N1B4_5701 0.41 -1.8 140,077 - -2.1 140,077 - Pf1N1B4_5701 0.41 -0.7 140,085 - -0.5 140,088 + Pf1N1B4_5701 0.48 -0.8 140,089 - Pf1N1B4_5701 0.48 -0.8 140,095 - Pf1N1B4_5701 0.52 +0.1 140,095 - Pf1N1B4_5701 0.52 -1.1 140,141 - Pf1N1B4_5701 0.80 +2.1 140,143 + Pf1N1B4_5701 0.81 +0.1 140,143 + Pf1N1B4_5701 0.81 -2.1 140,172 + -0.1 140,172 + +0.5 140,328 - +0.8 140,339 + -0.5 140,654 + Pf1N1B4_5703 0.41 -0.2 140,654 + Pf1N1B4_5703 0.41 +0.0 140,654 + Pf1N1B4_5703 0.41 +1.8 140,654 + Pf1N1B4_5703 0.41 -0.5 140,655 - Pf1N1B4_5703 0.41 -0.8 140,696 + Pf1N1B4_5703 0.47 -1.1 140,835 - Pf1N1B4_5703 0.66 -1.8 141,049 - -0.5
Or see this region's nucleotide sequence