Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5638 and Pf1N1B4_5639 are separated by 141 nucleotides Pf1N1B4_5639 and Pf1N1B4_5640 are separated by 39 nucleotides Pf1N1B4_5640 and Pf1N1B4_5641 are separated by 130 nucleotides
Pf1N1B4_5638 - 2-dehydro-3-deoxygluconate kinase (EC 2.7.1.45), at 68,461 to 69,393
_5638
Pf1N1B4_5639 - D-serine deaminase (EC 4.3.1.18), at 69,535 to 70,752
_5639
Pf1N1B4_5640 - Transcriptional regulator, IclR family, at 70,792 to 71,571
_5640
Pf1N1B4_5641 - Bona fide RidA/YjgF/TdcF/RutC subgroup, at 71,702 to 72,091
_5641
Position (kb)
69
70
71 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 68.799 kb on - strand, within Pf1N1B4_5638 at 68.972 kb on + strand at 68.984 kb on + strand, within Pf1N1B4_5638 at 69.765 kb on - strand, within Pf1N1B4_5639 at 69.798 kb on - strand, within Pf1N1B4_5639 at 69.942 kb on - strand, within Pf1N1B4_5639 at 69.961 kb on + strand, within Pf1N1B4_5639 at 69.962 kb on - strand, within Pf1N1B4_5639 at 69.962 kb on - strand, within Pf1N1B4_5639 at 69.965 kb on + strand, within Pf1N1B4_5639 at 69.965 kb on + strand, within Pf1N1B4_5639 at 69.966 kb on - strand, within Pf1N1B4_5639 at 69.966 kb on - strand at 69.966 kb on - strand at 69.966 kb on - strand, within Pf1N1B4_5639 at 69.966 kb on - strand, within Pf1N1B4_5639 at 70.040 kb on + strand, within Pf1N1B4_5639 at 70.040 kb on + strand at 70.334 kb on + strand, within Pf1N1B4_5639 at 70.516 kb on - strand at 70.748 kb on + strand at 70.803 kb on + strand at 70.847 kb on + strand at 70.856 kb on + strand at 70.856 kb on + strand at 70.857 kb on - strand at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.208 kb on + strand, within Pf1N1B4_5640 at 71.209 kb on - strand, within Pf1N1B4_5640 at 71.210 kb on + strand, within Pf1N1B4_5640 at 71.333 kb on + strand, within Pf1N1B4_5640 at 71.333 kb on + strand at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.334 kb on - strand, within Pf1N1B4_5640 at 71.352 kb on - strand, within Pf1N1B4_5640 at 71.590 kb on + strand at 71.590 kb on + strand at 71.591 kb on - strand at 71.600 kb on - strand at 71.637 kb on - strand at 71.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 68,799 - Pf1N1B4_5638 0.36 +0.4 68,972 + -0.5 68,984 + Pf1N1B4_5638 0.56 -0.2 69,765 - Pf1N1B4_5639 0.19 -0.3 69,798 - Pf1N1B4_5639 0.22 -0.5 69,942 - Pf1N1B4_5639 0.33 -0.8 69,961 + Pf1N1B4_5639 0.35 -0.5 69,962 - Pf1N1B4_5639 0.35 -0.5 69,962 - Pf1N1B4_5639 0.35 -0.5 69,965 + Pf1N1B4_5639 0.35 -1.3 69,965 + Pf1N1B4_5639 0.35 +0.8 69,966 - Pf1N1B4_5639 0.35 -1.4 69,966 - -1.5 69,966 - -1.5 69,966 - Pf1N1B4_5639 0.35 -0.7 69,966 - Pf1N1B4_5639 0.35 -0.7 70,040 + Pf1N1B4_5639 0.41 -0.8 70,040 + +0.1 70,334 + Pf1N1B4_5639 0.66 -1.5 70,516 - +0.5 70,748 + -0.3 70,803 + +0.5 70,847 + -1.3 70,856 + +1.8 70,856 + -1.0 70,857 - -2.5 71,208 + Pf1N1B4_5640 0.53 -0.8 71,208 + Pf1N1B4_5640 0.53 -0.5 71,208 + Pf1N1B4_5640 0.53 -0.5 71,209 - Pf1N1B4_5640 0.53 -0.1 71,210 + Pf1N1B4_5640 0.54 -0.5 71,333 + Pf1N1B4_5640 0.69 -1.1 71,333 + -0.1 71,334 - Pf1N1B4_5640 0.69 -0.2 71,334 - Pf1N1B4_5640 0.69 +1.3 71,334 - Pf1N1B4_5640 0.69 +0.9 71,352 - Pf1N1B4_5640 0.72 +0.0 71,590 + -1.5 71,590 + -0.7 71,591 - +0.1 71,600 - -1.8 71,637 - -1.5 71,719 - -1.1
Or see this region's nucleotide sequence