Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5559 and Pf1N1B4_5560 are separated by 324 nucleotides Pf1N1B4_5560 and Pf1N1B4_5561 are separated by 281 nucleotides Pf1N1B4_5561 and Pf1N1B4_5562 are separated by 16 nucleotides
Pf1N1B4_5559 - hypothetical protein, at 329,864 to 329,986
_5559
Pf1N1B4_5560 - hypothetical protein, at 330,311 to 330,520
_5560
Pf1N1B4_5561 - hypothetical protein, at 330,802 to 331,050
_5561
Pf1N1B4_5562 - DNA/RNA non-specific endonuclease, at 331,067 to 331,942
_5562
Position (kb) 330
331 Strain fitness (log2 ratio) -2
-1
0
1
2 at 329.342 kb on - strand at 329.455 kb on + strand at 329.455 kb on + strand at 329.455 kb on + strand at 329.468 kb on - strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.498 kb on + strand at 329.499 kb on - strand at 329.565 kb on - strand at 329.617 kb on + strand at 329.618 kb on - strand at 329.630 kb on + strand at 329.630 kb on + strand at 329.631 kb on - strand at 329.631 kb on - strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.689 kb on + strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.690 kb on - strand at 329.704 kb on + strand at 329.705 kb on - strand at 329.705 kb on - strand at 329.705 kb on - strand at 329.865 kb on + strand at 329.865 kb on + strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 329.866 kb on - strand at 330.312 kb on + strand at 330.357 kb on - strand, within Pf1N1B4_5560 at 330.357 kb on - strand at 330.474 kb on + strand, within Pf1N1B4_5560 at 330.474 kb on + strand, within Pf1N1B4_5560 at 330.535 kb on - strand at 330.563 kb on + strand at 330.563 kb on + strand at 330.803 kb on + strand at 330.803 kb on + strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.804 kb on - strand at 330.834 kb on + strand, within Pf1N1B4_5561 at 330.835 kb on - strand, within Pf1N1B4_5561 at 330.835 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.846 kb on - strand, within Pf1N1B4_5561 at 330.900 kb on - strand, within Pf1N1B4_5561 at 330.996 kb on - strand, within Pf1N1B4_5561 at 330.996 kb on - strand at 330.996 kb on - strand, within Pf1N1B4_5561 at 331.040 kb on + strand at 331.041 kb on - strand at 331.041 kb on - strand at 331.230 kb on + strand, within Pf1N1B4_5562 at 331.230 kb on + strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.231 kb on - strand, within Pf1N1B4_5562 at 331.302 kb on + strand, within Pf1N1B4_5562 at 331.302 kb on + strand, within Pf1N1B4_5562 at 331.435 kb on + strand, within Pf1N1B4_5562 at 331.436 kb on - strand, within Pf1N1B4_5562 at 331.436 kb on - strand, within Pf1N1B4_5562
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 329,342 - +1.4 329,455 + +0.7 329,455 + -0.7 329,455 + +0.3 329,468 - -0.4 329,498 + +1.1 329,498 + -0.5 329,498 + -1.2 329,498 + -0.1 329,499 - -0.5 329,565 - -0.2 329,617 + +0.3 329,618 - -0.7 329,630 + +0.6 329,630 + +0.1 329,631 - -0.7 329,631 - -0.1 329,689 + -0.1 329,689 + +0.7 329,689 + -0.7 329,689 + -1.7 329,690 - +0.4 329,690 - -0.1 329,690 - -0.1 329,690 - -1.7 329,690 - +0.9 329,704 + -0.1 329,705 - +0.4 329,705 - +0.2 329,705 - +0.3 329,865 + -0.1 329,865 + +1.9 329,866 - -1.1 329,866 - -0.8 329,866 - -0.1 329,866 - +1.3 329,866 - +0.5 329,866 - +0.5 329,866 - -1.1 329,866 - +0.6 330,312 + -0.1 330,357 - Pf1N1B4_5560 0.22 -1.2 330,357 - -0.1 330,474 + Pf1N1B4_5560 0.78 -1.7 330,474 + Pf1N1B4_5560 0.78 -0.1 330,535 - -1.1 330,563 + +0.5 330,563 + -1.7 330,803 + -0.6 330,803 + -2.4 330,804 - -0.1 330,804 - -1.1 330,804 - +0.3 330,804 - -1.7 330,804 - +0.9 330,804 - +1.4 330,804 - +0.9 330,804 - +0.5 330,804 - -0.5 330,804 - -0.8 330,804 - +0.9 330,804 - -0.4 330,804 - -0.2 330,804 - -0.3 330,834 + Pf1N1B4_5561 0.13 +1.5 330,835 - Pf1N1B4_5561 0.13 -0.7 330,835 - Pf1N1B4_5561 0.13 +0.3 330,846 - Pf1N1B4_5561 0.18 -1.1 330,846 - Pf1N1B4_5561 0.18 +0.0 330,846 - Pf1N1B4_5561 0.18 -2.1 330,846 - Pf1N1B4_5561 0.18 -0.1 330,900 - Pf1N1B4_5561 0.39 -1.1 330,996 - Pf1N1B4_5561 0.78 -2.4 330,996 - +0.9 330,996 - Pf1N1B4_5561 0.78 -0.7 331,040 + -1.1 331,041 - -0.6 331,041 - +0.5 331,230 + Pf1N1B4_5562 0.19 -1.4 331,230 + Pf1N1B4_5562 0.19 +0.9 331,231 - Pf1N1B4_5562 0.19 -0.8 331,231 - Pf1N1B4_5562 0.19 -2.1 331,231 - Pf1N1B4_5562 0.19 -0.1 331,302 + Pf1N1B4_5562 0.27 +0.5 331,302 + Pf1N1B4_5562 0.27 -2.4 331,435 + Pf1N1B4_5562 0.42 -0.5 331,436 - Pf1N1B4_5562 0.42 -1.7 331,436 - Pf1N1B4_5562 0.42 -0.3
Or see this region's nucleotide sequence