Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5551 and Pf1N1B4_5552 are separated by 182 nucleotides Pf1N1B4_5552 and Pf1N1B4_5553 are separated by 394 nucleotides
Pf1N1B4_5551 - hypothetical protein, at 323,086 to 324,072
_5551
Pf1N1B4_5552 - hypothetical protein, at 324,255 to 324,395
_5552
Pf1N1B4_5553 - Exodeoxyribonuclease I (EC 3.1.11.1), at 324,790 to 326,202
_5553
Position (kb) 324
325 Strain fitness (log2 ratio) -2
-1
0
1
2 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.464 kb on - strand, within Pf1N1B4_5551 at 323.498 kb on + strand, within Pf1N1B4_5551 at 323.499 kb on - strand, within Pf1N1B4_5551 at 323.564 kb on - strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand, within Pf1N1B4_5551 at 323.576 kb on + strand at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.577 kb on - strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.791 kb on + strand, within Pf1N1B4_5551 at 323.792 kb on - strand, within Pf1N1B4_5551 at 323.935 kb on + strand, within Pf1N1B4_5551 at 324.012 kb on + strand at 324.059 kb on + strand at 324.092 kb on + strand at 324.210 kb on - strand at 324.210 kb on - strand at 324.251 kb on + strand at 324.252 kb on - strand at 324.262 kb on + strand at 324.262 kb on + strand at 324.263 kb on - strand at 324.306 kb on + strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.307 kb on - strand, within Pf1N1B4_5552 at 324.335 kb on + strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.360 kb on - strand, within Pf1N1B4_5552 at 324.582 kb on + strand at 324.582 kb on + strand at 324.583 kb on - strand at 324.604 kb on - strand at 324.699 kb on + strand at 324.700 kb on - strand at 324.721 kb on + strand at 324.722 kb on - strand at 324.722 kb on - strand at 324.882 kb on + strand at 324.882 kb on + strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.883 kb on - strand at 324.926 kb on - strand at 325.004 kb on + strand at 325.005 kb on - strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.130 kb on + strand, within Pf1N1B4_5553 at 325.135 kb on - strand at 325.240 kb on + strand, within Pf1N1B4_5553 at 325.240 kb on + strand at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553 at 325.241 kb on - strand, within Pf1N1B4_5553
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 323,464 - Pf1N1B4_5551 0.38 +0.2 323,464 - Pf1N1B4_5551 0.38 -1.7 323,498 + Pf1N1B4_5551 0.42 +0.9 323,499 - Pf1N1B4_5551 0.42 -0.1 323,564 - Pf1N1B4_5551 0.48 -0.1 323,576 + Pf1N1B4_5551 0.50 -0.5 323,576 + Pf1N1B4_5551 0.50 -2.1 323,576 + +0.9 323,577 - Pf1N1B4_5551 0.50 -0.4 323,577 - Pf1N1B4_5551 0.50 -0.7 323,577 - Pf1N1B4_5551 0.50 -0.7 323,791 + Pf1N1B4_5551 0.71 -0.7 323,791 + Pf1N1B4_5551 0.71 -0.1 323,791 + Pf1N1B4_5551 0.71 -0.1 323,792 - Pf1N1B4_5551 0.72 -1.7 323,935 + Pf1N1B4_5551 0.86 +0.7 324,012 + -0.1 324,059 + +2.3 324,092 + -0.1 324,210 - -1.7 324,210 - +0.9 324,251 + +0.9 324,252 - -1.1 324,262 + -0.1 324,262 + -0.1 324,263 - -1.1 324,306 + Pf1N1B4_5552 0.36 -0.1 324,307 - Pf1N1B4_5552 0.37 -2.1 324,307 - Pf1N1B4_5552 0.37 -1.1 324,307 - Pf1N1B4_5552 0.37 +0.5 324,335 + Pf1N1B4_5552 0.57 -1.1 324,360 - Pf1N1B4_5552 0.74 -1.7 324,360 - Pf1N1B4_5552 0.74 +0.9 324,582 + -0.2 324,582 + -1.5 324,583 - -0.1 324,604 - -0.1 324,699 + -1.1 324,700 - +2.3 324,721 + -0.1 324,722 - +0.2 324,722 - -2.4 324,882 + +2.3 324,882 + -2.1 324,883 - -0.4 324,883 - -0.5 324,883 - -0.7 324,883 - -2.1 324,926 - -0.7 325,004 + +0.7 325,005 - Pf1N1B4_5553 0.15 -0.1 325,130 + Pf1N1B4_5553 0.24 +1.7 325,130 + Pf1N1B4_5553 0.24 +0.9 325,135 - -1.1 325,240 + Pf1N1B4_5553 0.32 -0.1 325,240 + +0.9 325,241 - Pf1N1B4_5553 0.32 +0.7 325,241 - Pf1N1B4_5553 0.32 -0.1 325,241 - Pf1N1B4_5553 0.32 +0.9 325,241 - Pf1N1B4_5553 0.32 +2.3 325,241 - Pf1N1B4_5553 0.32 +0.4
Or see this region's nucleotide sequence