Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5441 and Pf1N1B4_5442 are separated by 164 nucleotides Pf1N1B4_5442 and Pf1N1B4_5443 are separated by 655 nucleotides Pf1N1B4_5443 and Pf1N1B4_5444 are separated by 75 nucleotides Pf1N1B4_5444 and Pf1N1B4_5445 are separated by 493 nucleotides
Pf1N1B4_5441 - hypothetical protein, at 186,749 to 187,255
_5441
Pf1N1B4_5442 - hypothetical protein, at 187,420 to 187,551
_5442
Pf1N1B4_5443 - hypothetical protein, at 188,207 to 188,659
_5443
Pf1N1B4_5444 - hypothetical protein, at 188,735 to 188,938
_5444
Pf1N1B4_5445 - hypothetical protein, at 189,432 to 190,823
_5445
Position (kb) 188
189 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 187.328 kb on + strand at 187.328 kb on + strand at 187.329 kb on - strand at 187.461 kb on - strand, within Pf1N1B4_5442 at 187.461 kb on - strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.524 kb on + strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.525 kb on - strand, within Pf1N1B4_5442 at 187.570 kb on - strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.638 kb on + strand at 187.639 kb on - strand at 187.648 kb on + strand at 187.648 kb on + strand at 187.648 kb on + strand at 187.649 kb on - strand at 187.725 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.769 kb on + strand at 187.770 kb on - strand at 187.770 kb on - strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.804 kb on + strand at 187.805 kb on - strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.816 kb on + strand at 187.817 kb on - strand at 187.817 kb on - strand at 187.817 kb on - strand at 187.831 kb on + strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.832 kb on - strand at 187.891 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.900 kb on + strand at 187.940 kb on - strand at 187.940 kb on - strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.947 kb on + strand at 187.948 kb on - strand at 187.948 kb on - strand at 187.948 kb on - strand at 187.963 kb on + strand at 187.963 kb on + strand at 187.969 kb on - strand at 187.971 kb on + strand at 187.971 kb on + strand at 187.972 kb on - strand at 187.993 kb on + strand at 188.032 kb on + strand at 188.032 kb on + strand at 188.033 kb on - strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.094 kb on + strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.095 kb on - strand at 188.199 kb on - strand at 188.202 kb on + strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.203 kb on - strand at 188.205 kb on + strand at 188.206 kb on - strand at 188.206 kb on - strand at 188.209 kb on - strand at 188.209 kb on - strand at 188.238 kb on + strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.239 kb on - strand at 188.254 kb on - strand, within Pf1N1B4_5443 at 188.254 kb on - strand, within Pf1N1B4_5443 at 188.288 kb on - strand, within Pf1N1B4_5443 at 188.323 kb on + strand, within Pf1N1B4_5443 at 188.323 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.427 kb on + strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.428 kb on - strand, within Pf1N1B4_5443 at 188.529 kb on - strand, within Pf1N1B4_5443 at 188.529 kb on - strand, within Pf1N1B4_5443 at 188.542 kb on - strand, within Pf1N1B4_5443 at 188.542 kb on - strand, within Pf1N1B4_5443 at 188.677 kb on + strand at 188.678 kb on - strand at 188.681 kb on + strand at 188.682 kb on - strand at 188.682 kb on - strand at 188.682 kb on - strand at 188.682 kb on - strand at 188.695 kb on - strand at 188.716 kb on + strand at 189.006 kb on + strand at 189.124 kb on + strand at 189.124 kb on + strand at 189.124 kb on + strand at 189.125 kb on - strand at 189.125 kb on - strand at 189.194 kb on + strand at 189.195 kb on - strand at 189.195 kb on - strand at 189.242 kb on + strand at 189.242 kb on + strand at 189.242 kb on + strand at 189.242 kb on + strand at 189.242 kb on + strand at 189.242 kb on + strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.243 kb on - strand at 189.395 kb on + strand at 189.395 kb on + strand at 189.395 kb on + strand at 189.395 kb on + strand at 189.395 kb on + strand at 189.395 kb on + strand at 189.396 kb on - strand at 189.396 kb on - strand at 189.396 kb on - strand at 189.422 kb on - strand at 189.482 kb on - strand at 189.493 kb on + strand at 189.494 kb on - strand at 189.494 kb on - strand at 189.558 kb on + strand at 189.558 kb on + strand at 189.558 kb on + strand at 189.559 kb on - strand at 189.559 kb on - strand at 189.559 kb on - strand at 189.559 kb on - strand at 189.606 kb on - strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.617 kb on + strand, within Pf1N1B4_5445 at 189.618 kb on - strand, within Pf1N1B4_5445 at 189.618 kb on - strand, within Pf1N1B4_5445 at 189.618 kb on - strand, within Pf1N1B4_5445 at 189.618 kb on - strand, within Pf1N1B4_5445 at 189.618 kb on - strand, within Pf1N1B4_5445 at 189.635 kb on - strand, within Pf1N1B4_5445 at 189.635 kb on - strand, within Pf1N1B4_5445
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 187,328 + -1.5 187,328 + +2.7 187,329 - -2.1 187,461 - Pf1N1B4_5442 0.31 -0.3 187,461 - Pf1N1B4_5442 0.31 +0.9 187,524 + Pf1N1B4_5442 0.79 -2.4 187,524 + Pf1N1B4_5442 0.79 -0.8 187,524 + Pf1N1B4_5442 0.79 +0.1 187,524 + Pf1N1B4_5442 0.79 +0.3 187,524 + Pf1N1B4_5442 0.79 -2.1 187,524 + Pf1N1B4_5442 0.79 +2.5 187,525 - Pf1N1B4_5442 0.80 -0.1 187,525 - Pf1N1B4_5442 0.80 -0.3 187,525 - Pf1N1B4_5442 0.80 -1.1 187,570 - -2.4 187,638 + -0.3 187,638 + -1.1 187,638 + -2.9 187,638 + -0.7 187,638 + -1.1 187,638 + +0.0 187,639 - -0.1 187,648 + +0.2 187,648 + +1.3 187,648 + +0.9 187,649 - +2.7 187,725 + -0.1 187,769 + -1.1 187,769 + +0.6 187,769 + -1.7 187,769 + -1.1 187,770 - +1.7 187,770 - +0.3 187,804 + -0.5 187,804 + -0.1 187,804 + +0.9 187,804 + +0.7 187,804 + -0.9 187,805 - +1.5 187,816 + -0.2 187,816 + -0.1 187,816 + -0.4 187,816 + +0.4 187,817 - -0.7 187,817 - +0.9 187,817 - -1.1 187,831 + -0.1 187,832 - -0.7 187,832 - -1.7 187,832 - -0.0 187,891 + +0.7 187,900 + -1.7 187,900 + +1.7 187,900 + -0.1 187,900 + +0.9 187,940 - -0.1 187,940 - -0.1 187,947 + -1.7 187,947 + -0.1 187,947 + +0.3 187,947 + -2.4 187,947 + -0.1 187,947 + -1.1 187,947 + -1.7 187,947 + -0.9 187,947 + -0.1 187,947 + +0.9 187,947 + +1.9 187,948 - -1.1 187,948 - +0.2 187,948 - +0.5 187,963 + -0.3 187,963 + -1.1 187,969 - -1.7 187,971 + -0.8 187,971 + -0.9 187,972 - -0.1 187,993 + -0.1 188,032 + -0.1 188,032 + -0.1 188,033 - +0.5 188,094 + +0.9 188,094 + -1.7 188,094 + +0.3 188,094 + -1.7 188,094 + -1.1 188,094 + +0.3 188,094 + -0.1 188,094 + -0.7 188,095 - -0.1 188,095 - -0.8 188,095 - -2.1 188,095 - +1.3 188,095 - -0.2 188,199 - -1.1 188,202 + +1.5 188,203 - -0.4 188,203 - -2.7 188,203 - +0.9 188,203 - -0.5 188,205 + -1.8 188,206 - +3.5 188,206 - -3.2 188,209 - -1.1 188,209 - -0.7 188,238 + -0.1 188,239 - -0.3 188,239 - -0.8 188,239 - +1.5 188,239 - +0.3 188,239 - -2.7 188,254 - Pf1N1B4_5443 0.10 -0.6 188,254 - Pf1N1B4_5443 0.10 -2.1 188,288 - Pf1N1B4_5443 0.18 -1.1 188,323 + Pf1N1B4_5443 0.26 -1.1 188,323 + Pf1N1B4_5443 0.26 +0.9 188,427 + Pf1N1B4_5443 0.49 +0.3 188,427 + Pf1N1B4_5443 0.49 +1.5 188,427 + Pf1N1B4_5443 0.49 -1.8 188,428 - Pf1N1B4_5443 0.49 -1.1 188,428 - Pf1N1B4_5443 0.49 -1.7 188,428 - Pf1N1B4_5443 0.49 -1.7 188,529 - Pf1N1B4_5443 0.71 -0.1 188,529 - Pf1N1B4_5443 0.71 -2.4 188,542 - Pf1N1B4_5443 0.74 -1.7 188,542 - Pf1N1B4_5443 0.74 -0.3 188,677 + +1.5 188,678 - +0.2 188,681 + -0.4 188,682 - -0.4 188,682 - -1.7 188,682 - -0.1 188,682 - +0.1 188,695 - -0.1 188,716 + +1.3 189,006 + -0.7 189,124 + -0.7 189,124 + +0.5 189,124 + -1.4 189,125 - +0.9 189,125 - +1.4 189,194 + -0.7 189,195 - -0.6 189,195 - +1.9 189,242 + -2.4 189,242 + -0.1 189,242 + +0.5 189,242 + -0.5 189,242 + -2.1 189,242 + -2.1 189,243 - -1.3 189,243 - +1.3 189,243 - +0.2 189,243 - -1.7 189,243 - -0.3 189,243 - -0.1 189,243 - +0.9 189,243 - +0.5 189,395 + +1.1 189,395 + +1.5 189,395 + +0.5 189,395 + -0.3 189,395 + -1.1 189,395 + -2.1 189,396 - -0.1 189,396 - -0.1 189,396 - +0.5 189,422 - +1.3 189,482 - +1.3 189,493 + +0.9 189,494 - -1.1 189,494 - +0.5 189,558 + +0.9 189,558 + -1.0 189,558 + +0.7 189,559 - -0.4 189,559 - +0.1 189,559 - -0.1 189,559 - -1.1 189,606 - Pf1N1B4_5445 0.12 -0.1 189,617 + Pf1N1B4_5445 0.13 -1.4 189,617 + -0.7 189,617 + Pf1N1B4_5445 0.13 -0.7 189,617 + Pf1N1B4_5445 0.13 +0.7 189,617 + Pf1N1B4_5445 0.13 -0.5 189,617 + Pf1N1B4_5445 0.13 +1.7 189,617 + Pf1N1B4_5445 0.13 -1.1 189,617 + Pf1N1B4_5445 0.13 +0.9 189,617 + Pf1N1B4_5445 0.13 -1.2 189,618 - Pf1N1B4_5445 0.13 +0.5 189,618 - Pf1N1B4_5445 0.13 -0.4 189,618 - Pf1N1B4_5445 0.13 -1.7 189,618 - Pf1N1B4_5445 0.13 +1.4 189,618 - Pf1N1B4_5445 0.13 +0.2 189,635 - Pf1N1B4_5445 0.15 -0.4 189,635 - Pf1N1B4_5445 0.15 +0.2
Or see this region's nucleotide sequence