Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5321 and Pf1N1B4_5322 are separated by 744 nucleotides Pf1N1B4_5322 and Pf1N1B4_5323 are separated by 96 nucleotides
Pf1N1B4_5321 - hypothetical protein, at 70,641 to 70,970
_5321
Pf1N1B4_5322 - hypothetical protein, at 71,715 to 71,900
_5322
Pf1N1B4_5323 - hypothetical protein, at 71,997 to 72,953
_5323
Position (kb)
71
72 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.722 kb on - strand, within Pf1N1B4_5321 at 70.824 kb on - strand, within Pf1N1B4_5321 at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.058 kb on + strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.059 kb on - strand at 71.092 kb on + strand at 71.092 kb on + strand at 71.093 kb on - strand at 71.114 kb on + strand at 71.114 kb on + strand at 71.115 kb on - strand at 71.131 kb on - strand at 71.139 kb on - strand at 71.229 kb on + strand at 71.335 kb on + strand at 71.335 kb on + strand at 71.336 kb on - strand at 71.356 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.358 kb on + strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.359 kb on - strand at 71.433 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.437 kb on + strand at 71.481 kb on - strand at 71.499 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.669 kb on + strand at 71.670 kb on - strand at 71.670 kb on - strand at 71.715 kb on - strand at 71.752 kb on + strand, within Pf1N1B4_5322 at 71.753 kb on - strand, within Pf1N1B4_5322 at 71.763 kb on - strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.880 kb on + strand, within Pf1N1B4_5322 at 71.898 kb on + strand at 71.899 kb on - strand at 71.913 kb on + strand at 71.917 kb on + strand at 71.917 kb on + strand at 71.918 kb on - strand at 71.926 kb on + strand at 71.926 kb on + strand at 71.927 kb on - strand at 71.975 kb on + strand at 72.165 kb on - strand, within Pf1N1B4_5323 at 72.268 kb on + strand, within Pf1N1B4_5323 at 72.269 kb on - strand, within Pf1N1B4_5323 at 72.411 kb on - strand, within Pf1N1B4_5323 at 72.495 kb on + strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.496 kb on - strand, within Pf1N1B4_5323 at 72.515 kb on - strand, within Pf1N1B4_5323 at 72.529 kb on + strand, within Pf1N1B4_5323 at 72.529 kb on + strand, within Pf1N1B4_5323 at 72.530 kb on - strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.531 kb on + strand, within Pf1N1B4_5323 at 72.532 kb on - strand, within Pf1N1B4_5323 at 72.575 kb on - strand, within Pf1N1B4_5323 at 72.668 kb on + strand, within Pf1N1B4_5323 at 72.788 kb on + strand, within Pf1N1B4_5323 at 72.865 kb on + strand at 72.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 70,722 - Pf1N1B4_5321 0.25 -1.1 70,722 - Pf1N1B4_5321 0.25 -0.1 70,722 - Pf1N1B4_5321 0.25 -0.9 70,824 - Pf1N1B4_5321 0.55 -1.4 71,058 + -2.7 71,058 + -1.2 71,058 + -0.1 71,058 + -0.7 71,058 + +1.5 71,058 + +0.3 71,059 - -0.1 71,059 - -1.1 71,059 - +0.2 71,092 + -0.7 71,092 + -0.7 71,093 - -0.1 71,114 + -0.4 71,114 + -1.1 71,115 - -1.1 71,131 - +0.2 71,139 - +0.6 71,229 + -1.7 71,335 + -0.6 71,335 + +0.7 71,336 - -0.1 71,356 + -0.1 71,358 + -1.9 71,358 + -2.1 71,358 + +1.2 71,358 + +0.7 71,358 + -0.7 71,358 + +0.9 71,358 + -1.7 71,359 - +0.9 71,359 - +0.9 71,359 - -0.7 71,359 - -2.1 71,359 - -0.1 71,359 - +1.2 71,433 + -0.1 71,437 + +0.7 71,437 + -0.1 71,437 + -2.4 71,481 - +0.2 71,499 + -1.1 71,669 + +0.2 71,669 + -1.4 71,669 + +1.2 71,669 + +2.5 71,670 - -1.2 71,670 - -0.1 71,715 - +0.5 71,752 + Pf1N1B4_5322 0.20 -0.7 71,753 - Pf1N1B4_5322 0.20 -2.7 71,763 - Pf1N1B4_5322 0.26 -1.7 71,880 + Pf1N1B4_5322 0.89 +1.9 71,880 + Pf1N1B4_5322 0.89 -1.0 71,898 + +1.2 71,899 - +0.5 71,913 + +0.9 71,917 + +0.8 71,917 + -2.1 71,918 - -1.7 71,926 + +1.7 71,926 + -0.1 71,927 - +0.9 71,975 + -0.8 72,165 - Pf1N1B4_5323 0.18 -0.5 72,268 + Pf1N1B4_5323 0.28 -0.7 72,269 - Pf1N1B4_5323 0.28 -0.7 72,411 - Pf1N1B4_5323 0.43 -1.1 72,495 + Pf1N1B4_5323 0.52 -1.2 72,496 - Pf1N1B4_5323 0.52 -0.3 72,496 - Pf1N1B4_5323 0.52 -0.8 72,496 - Pf1N1B4_5323 0.52 -0.3 72,515 - Pf1N1B4_5323 0.54 -0.1 72,529 + Pf1N1B4_5323 0.56 -0.1 72,529 + Pf1N1B4_5323 0.56 -0.2 72,530 - Pf1N1B4_5323 0.56 -2.4 72,531 + Pf1N1B4_5323 0.56 +0.6 72,531 + Pf1N1B4_5323 0.56 -1.1 72,531 + Pf1N1B4_5323 0.56 -1.4 72,532 - Pf1N1B4_5323 0.56 -1.1 72,575 - Pf1N1B4_5323 0.60 +2.1 72,668 + Pf1N1B4_5323 0.70 -0.9 72,788 + Pf1N1B4_5323 0.83 -1.5 72,865 + -2.7 72,866 - -0.1
Or see this region's nucleotide sequence