Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5289 and Pf1N1B4_5290 are separated by 351 nucleotides Pf1N1B4_5290 and Pf1N1B4_5291 are separated by 290 nucleotides
Pf1N1B4_5289 - hypothetical protein, at 43,736 to 44,062
_5289
Pf1N1B4_5290 - hypothetical protein, at 44,414 to 44,644
_5290
Pf1N1B4_5291 - Putative reductase, at 44,935 to 46,248
_5291
Position (kb) 43
44
45 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 42.747 kb on + strand at 42.748 kb on - strand at 42.784 kb on + strand at 42.814 kb on + strand at 42.849 kb on + strand at 42.940 kb on + strand at 42.940 kb on + strand at 43.041 kb on - strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.042 kb on + strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.043 kb on - strand at 43.099 kb on - strand at 43.099 kb on - strand at 43.104 kb on - strand at 43.155 kb on - strand at 43.332 kb on - strand at 43.413 kb on + strand at 43.413 kb on + strand at 43.510 kb on + strand at 43.606 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.765 kb on + strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.766 kb on - strand at 43.920 kb on - strand, within Pf1N1B4_5289 at 43.920 kb on - strand, within Pf1N1B4_5289 at 43.944 kb on + strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.945 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 43.967 kb on - strand, within Pf1N1B4_5289 at 44.127 kb on - strand at 44.255 kb on + strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.256 kb on - strand at 44.275 kb on + strand at 44.415 kb on + strand at 44.650 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 42,747 + -2.3 42,748 - +1.9 42,784 + -0.4 42,814 + -0.5 42,849 + -1.1 42,940 + +1.9 42,940 + -0.1 43,041 - -1.1 43,042 + -1.7 43,042 + -0.7 43,042 + -2.1 43,042 + -1.1 43,042 + -2.1 43,042 + -1.7 43,043 - -0.8 43,043 - +0.1 43,043 - -0.4 43,043 - +1.9 43,099 - +0.9 43,099 - +1.2 43,104 - -0.8 43,155 - -0.1 43,332 - +0.9 43,413 + +0.9 43,413 + -0.7 43,510 + -0.3 43,606 + -0.7 43,765 + +1.5 43,765 + +2.9 43,765 + -0.5 43,765 + -0.5 43,765 + -0.1 43,766 - -1.1 43,766 - +0.3 43,766 - -0.1 43,766 - +1.2 43,766 - +0.9 43,766 - -0.7 43,920 - Pf1N1B4_5289 0.56 -0.7 43,920 - Pf1N1B4_5289 0.56 -1.1 43,944 + Pf1N1B4_5289 0.64 -1.1 43,945 - Pf1N1B4_5289 0.64 -1.1 43,945 - Pf1N1B4_5289 0.64 -1.7 43,967 - Pf1N1B4_5289 0.71 -0.1 43,967 - Pf1N1B4_5289 0.71 -0.7 44,127 - -0.5 44,255 + -0.1 44,256 - -1.7 44,256 - -0.1 44,256 - +0.2 44,275 + -0.1 44,415 + +0.2 44,650 - -1.4
Or see this region's nucleotide sequence