Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5257 and Pf1N1B4_5258 are separated by 78 nucleotides Pf1N1B4_5258 and Pf1N1B4_5259 are separated by 314 nucleotides
Pf1N1B4_5257 - hypothetical protein, at 16,007 to 16,306
_5257
Pf1N1B4_5258 - hypothetical protein, at 16,385 to 16,576
_5258
Pf1N1B4_5259 - hypothetical protein, at 16,891 to 17,250
_5259
Position (kb)
16
17
18 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.037 kb on + strand at 16.037 kb on + strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.038 kb on - strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.213 kb on + strand, within Pf1N1B4_5257 at 16.214 kb on - strand at 16.214 kb on - strand, within Pf1N1B4_5257 at 16.268 kb on + strand, within Pf1N1B4_5257 at 16.268 kb on + strand, within Pf1N1B4_5257 at 16.280 kb on + strand at 16.280 kb on + strand at 16.280 kb on + strand at 16.384 kb on + strand at 16.384 kb on + strand at 16.384 kb on + strand at 16.409 kb on + strand, within Pf1N1B4_5258 at 16.434 kb on + strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.435 kb on - strand, within Pf1N1B4_5258 at 16.509 kb on + strand, within Pf1N1B4_5258 at 16.510 kb on - strand, within Pf1N1B4_5258 at 16.510 kb on - strand, within Pf1N1B4_5258 at 16.563 kb on - strand at 16.575 kb on - strand at 16.623 kb on + strand at 16.623 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.627 kb on + strand at 16.628 kb on - strand at 16.628 kb on - strand at 16.648 kb on + strand at 16.648 kb on + strand at 16.676 kb on + strand at 16.676 kb on + strand at 16.676 kb on + strand at 16.747 kb on + strand at 16.761 kb on + strand at 16.762 kb on - strand at 16.809 kb on - strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.918 kb on + strand at 16.919 kb on - strand at 16.940 kb on + strand, within Pf1N1B4_5259 at 16.940 kb on + strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 16.941 kb on - strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.150 kb on + strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.151 kb on - strand, within Pf1N1B4_5259 at 17.163 kb on + strand, within Pf1N1B4_5259 at 17.164 kb on - strand, within Pf1N1B4_5259 at 17.244 kb on + strand at 17.244 kb on + strand at 17.245 kb on - strand at 17.268 kb on + strand at 17.439 kb on - strand at 17.459 kb on + strand at 17.586 kb on + strand at 17.595 kb on - strand at 17.620 kb on - strand at 17.620 kb on - strand at 17.684 kb on + strand at 17.684 kb on + strand at 17.685 kb on - strand at 17.700 kb on - strand at 17.718 kb on - strand at 17.718 kb on - strand at 17.750 kb on + strand at 17.751 kb on - strand at 17.861 kb on - strand at 18.136 kb on + strand at 18.192 kb on + strand at 18.192 kb on + strand at 18.193 kb on - strand at 18.244 kb on + strand at 18.248 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 16,037 + Pf1N1B4_5257 0.10 -0.6 16,037 + Pf1N1B4_5257 0.10 -2.4 16,037 + +1.5 16,037 + Pf1N1B4_5257 0.10 -1.7 16,038 - Pf1N1B4_5257 0.10 -1.2 16,038 - Pf1N1B4_5257 0.10 -0.4 16,038 - Pf1N1B4_5257 0.10 +0.4 16,038 - Pf1N1B4_5257 0.10 -3.4 16,213 + Pf1N1B4_5257 0.69 -0.4 16,213 + Pf1N1B4_5257 0.69 -0.9 16,213 + Pf1N1B4_5257 0.69 +0.2 16,213 + Pf1N1B4_5257 0.69 -0.8 16,214 - -1.7 16,214 - Pf1N1B4_5257 0.69 +0.5 16,268 + Pf1N1B4_5257 0.87 -0.1 16,268 + Pf1N1B4_5257 0.87 -0.7 16,280 + +0.5 16,280 + -0.1 16,280 + -0.4 16,384 + -0.3 16,384 + -0.3 16,384 + -0.7 16,409 + Pf1N1B4_5258 0.12 +0.5 16,434 + Pf1N1B4_5258 0.26 -0.5 16,435 - Pf1N1B4_5258 0.26 +1.7 16,435 - Pf1N1B4_5258 0.26 -2.1 16,435 - Pf1N1B4_5258 0.26 +0.3 16,435 - Pf1N1B4_5258 0.26 -0.5 16,435 - Pf1N1B4_5258 0.26 -0.1 16,435 - Pf1N1B4_5258 0.26 -1.4 16,509 + Pf1N1B4_5258 0.65 -0.3 16,510 - Pf1N1B4_5258 0.65 +0.9 16,510 - Pf1N1B4_5258 0.65 -0.1 16,563 - +0.5 16,575 - -1.1 16,623 + -2.9 16,623 + -0.3 16,627 + +0.5 16,627 + -1.1 16,627 + -0.9 16,627 + -1.1 16,627 + -2.1 16,627 + +1.9 16,627 + -0.6 16,628 - -1.1 16,628 - -0.1 16,648 + -0.4 16,648 + +1.5 16,676 + -0.1 16,676 + -1.0 16,676 + -1.7 16,747 + -0.2 16,761 + -0.7 16,762 - -1.1 16,809 - -0.2 16,918 + +0.9 16,918 + -0.1 16,918 + +1.4 16,918 + +1.2 16,918 + +0.7 16,919 - -1.7 16,940 + Pf1N1B4_5259 0.14 +0.5 16,940 + Pf1N1B4_5259 0.14 -1.1 16,941 - Pf1N1B4_5259 0.14 -0.1 16,941 - Pf1N1B4_5259 0.14 -1.1 16,941 - Pf1N1B4_5259 0.14 -0.8 17,150 + Pf1N1B4_5259 0.72 -1.9 17,150 + Pf1N1B4_5259 0.72 -0.8 17,150 + Pf1N1B4_5259 0.72 -0.1 17,150 + Pf1N1B4_5259 0.72 -1.8 17,150 + Pf1N1B4_5259 0.72 -1.7 17,150 + Pf1N1B4_5259 0.72 -3.1 17,150 + Pf1N1B4_5259 0.72 -1.7 17,151 - Pf1N1B4_5259 0.72 -0.8 17,151 - Pf1N1B4_5259 0.72 -2.1 17,151 - Pf1N1B4_5259 0.72 -1.7 17,151 - Pf1N1B4_5259 0.72 -0.1 17,163 + Pf1N1B4_5259 0.76 -0.5 17,164 - Pf1N1B4_5259 0.76 +0.5 17,244 + -0.1 17,244 + -0.1 17,245 - -1.1 17,268 + +1.2 17,439 - -0.3 17,459 + +0.5 17,586 + -1.5 17,595 - +0.5 17,620 - +0.3 17,620 - +0.4 17,684 + -1.4 17,684 + -0.1 17,685 - -0.1 17,700 - +0.5 17,718 - -0.7 17,718 - -0.1 17,750 + +0.7 17,751 - +0.7 17,861 - -1.9 18,136 + +1.5 18,192 + +0.6 18,192 + -0.1 18,193 - +1.5 18,244 + -0.8 18,248 - -0.6
Or see this region's nucleotide sequence