Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4958 and Pf1N1B4_4959 are separated by 3 nucleotides Pf1N1B4_4959 and Pf1N1B4_4960 overlap by 4 nucleotides Pf1N1B4_4960 and Pf1N1B4_4961 are separated by 98 nucleotides
Pf1N1B4_4958 - Protein containing domains DUF404, DUF407, at 5,498,657 to 5,499,988
_4958
Pf1N1B4_4959 - Protein containing domains DUF403, at 5,499,992 to 5,500,942
_4959
Pf1N1B4_4960 - Protein containing transglutaminase-like domain, putative cysteine protease, at 5,500,939 to 5,501,736
_4960
Pf1N1B4_4961 - FIG00441227: hypothetical protein, at 5,501,835 to 5,502,554
_4961
Position (kb)
5500
5501
5502 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5499.986 kb on + strand at 5499.987 kb on - strand at 5499.987 kb on - strand at 5499.987 kb on - strand at 5500.170 kb on - strand, within Pf1N1B4_4959 at 5500.250 kb on + strand, within Pf1N1B4_4959 at 5500.250 kb on + strand, within Pf1N1B4_4959 at 5500.652 kb on + strand, within Pf1N1B4_4959 at 5500.652 kb on + strand, within Pf1N1B4_4959 at 5500.652 kb on + strand, within Pf1N1B4_4959 at 5500.653 kb on - strand, within Pf1N1B4_4959 at 5500.925 kb on + strand at 5500.925 kb on + strand at 5500.938 kb on + strand at 5500.938 kb on + strand at 5500.969 kb on + strand at 5500.969 kb on + strand at 5500.970 kb on - strand at 5500.970 kb on - strand at 5500.970 kb on - strand at 5501.353 kb on + strand, within Pf1N1B4_4960 at 5501.479 kb on + strand, within Pf1N1B4_4960 at 5501.479 kb on + strand at 5501.480 kb on - strand, within Pf1N1B4_4960 at 5501.480 kb on - strand, within Pf1N1B4_4960 at 5501.563 kb on + strand, within Pf1N1B4_4960 at 5501.564 kb on - strand, within Pf1N1B4_4960 at 5501.824 kb on - strand at 5501.824 kb on - strand at 5501.824 kb on - strand at 5501.835 kb on - strand at 5502.057 kb on + strand at 5502.268 kb on - strand, within Pf1N1B4_4961 at 5502.377 kb on - strand, within Pf1N1B4_4961 at 5502.436 kb on - strand, within Pf1N1B4_4961 at 5502.459 kb on + strand, within Pf1N1B4_4961 at 5502.459 kb on + strand, within Pf1N1B4_4961 at 5502.537 kb on + strand at 5502.537 kb on + strand at 5502.537 kb on + strand at 5502.537 kb on + strand at 5502.538 kb on - strand at 5502.538 kb on - strand at 5502.538 kb on - strand at 5502.557 kb on + strand at 5502.657 kb on + strand at 5502.686 kb on - strand at 5502.729 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,499,986 + +0.3 5,499,987 - -0.3 5,499,987 - -2.0 5,499,987 - -1.7 5,500,170 - Pf1N1B4_4959 0.19 -0.4 5,500,250 + Pf1N1B4_4959 0.27 -1.7 5,500,250 + Pf1N1B4_4959 0.27 -3.3 5,500,652 + Pf1N1B4_4959 0.69 -0.9 5,500,652 + Pf1N1B4_4959 0.69 +0.3 5,500,652 + Pf1N1B4_4959 0.69 +1.6 5,500,653 - Pf1N1B4_4959 0.70 +0.3 5,500,925 + -0.1 5,500,925 + -0.5 5,500,938 + -0.2 5,500,938 + -1.7 5,500,969 + -0.7 5,500,969 + +2.1 5,500,970 - -1.7 5,500,970 - -0.1 5,500,970 - +0.6 5,501,353 + Pf1N1B4_4960 0.52 -1.7 5,501,479 + Pf1N1B4_4960 0.68 -2.7 5,501,479 + -0.7 5,501,480 - Pf1N1B4_4960 0.68 +0.9 5,501,480 - Pf1N1B4_4960 0.68 -2.3 5,501,563 + Pf1N1B4_4960 0.78 -1.7 5,501,564 - Pf1N1B4_4960 0.78 -0.7 5,501,824 - +0.5 5,501,824 - -1.7 5,501,824 - -0.7 5,501,835 - -0.3 5,502,057 + -0.7 5,502,268 - Pf1N1B4_4961 0.60 +0.3 5,502,377 - Pf1N1B4_4961 0.75 +1.3 5,502,436 - Pf1N1B4_4961 0.83 -0.1 5,502,459 + Pf1N1B4_4961 0.87 +0.6 5,502,459 + Pf1N1B4_4961 0.87 -2.5 5,502,537 + +0.3 5,502,537 + +0.2 5,502,537 + -2.3 5,502,537 + +0.9 5,502,538 - -0.2 5,502,538 - -1.3 5,502,538 - -0.1 5,502,557 + +0.1 5,502,657 + +1.3 5,502,686 - +1.3 5,502,729 - -1.7
Or see this region's nucleotide sequence