Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4822 and Pf1N1B4_4823 overlap by 4 nucleotides Pf1N1B4_4823 and Pf1N1B4_4824 are separated by 288 nucleotides Pf1N1B4_4824 and Pf1N1B4_4825 overlap by 4 nucleotides
Pf1N1B4_4822 - Paraquat-inducible protein A, at 5,326,381 to 5,327,061
_4822
Pf1N1B4_4823 - Paraquat-inducible protein A, at 5,327,058 to 5,327,654
_4823
Pf1N1B4_4824 - Gamma-glutamyl-putrescine oxidase (EC1.4.3.-), at 5,327,943 to 5,329,244
_4824
Pf1N1B4_4825 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,329,241 to 5,330,329
_4825
Position (kb)
5327
5328
5329
5330 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 5327.061 kb on + strand at 5327.082 kb on + strand at 5327.083 kb on - strand at 5327.151 kb on + strand, within Pf1N1B4_4823 at 5327.154 kb on + strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.155 kb on - strand, within Pf1N1B4_4823 at 5327.369 kb on + strand, within Pf1N1B4_4823 at 5327.461 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.564 kb on + strand, within Pf1N1B4_4823 at 5327.565 kb on - strand, within Pf1N1B4_4823 at 5327.605 kb on + strand at 5327.649 kb on - strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.702 kb on + strand at 5327.703 kb on - strand at 5327.728 kb on + strand at 5327.729 kb on - strand at 5327.729 kb on - strand at 5327.756 kb on + strand at 5327.756 kb on + strand at 5327.757 kb on - strand at 5327.887 kb on + strand at 5327.888 kb on - strand at 5327.888 kb on - strand at 5328.229 kb on + strand at 5328.284 kb on + strand, within Pf1N1B4_4824 at 5328.285 kb on - strand, within Pf1N1B4_4824 at 5328.287 kb on - strand, within Pf1N1B4_4824 at 5328.319 kb on + strand, within Pf1N1B4_4824 at 5328.464 kb on - strand at 5328.721 kb on + strand at 5328.721 kb on + strand at 5328.949 kb on + strand, within Pf1N1B4_4824 at 5328.973 kb on + strand, within Pf1N1B4_4824 at 5329.208 kb on - strand at 5329.211 kb on - strand at 5329.234 kb on + strand at 5329.318 kb on - strand at 5329.348 kb on - strand at 5329.361 kb on - strand, within Pf1N1B4_4825 at 5329.361 kb on - strand, within Pf1N1B4_4825 at 5329.361 kb on - strand, within Pf1N1B4_4825 at 5329.383 kb on + strand, within Pf1N1B4_4825 at 5329.383 kb on + strand, within Pf1N1B4_4825 at 5329.383 kb on + strand, within Pf1N1B4_4825 at 5329.384 kb on - strand, within Pf1N1B4_4825 at 5329.384 kb on - strand at 5329.686 kb on + strand, within Pf1N1B4_4825 at 5329.695 kb on + strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.732 kb on + strand, within Pf1N1B4_4825 at 5329.773 kb on + strand at 5330.172 kb on + strand, within Pf1N1B4_4825 at 5330.173 kb on - strand, within Pf1N1B4_4825 at 5330.229 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,327,061 + -0.7 5,327,082 + -1.4 5,327,083 - -0.7 5,327,151 + Pf1N1B4_4823 0.16 -0.7 5,327,154 + Pf1N1B4_4823 0.16 -1.7 5,327,155 - Pf1N1B4_4823 0.16 -1.7 5,327,155 - Pf1N1B4_4823 0.16 -0.7 5,327,155 - Pf1N1B4_4823 0.16 -0.3 5,327,155 - Pf1N1B4_4823 0.16 -1.7 5,327,369 + Pf1N1B4_4823 0.52 -0.0 5,327,461 + Pf1N1B4_4823 0.68 -0.1 5,327,564 + Pf1N1B4_4823 0.85 +0.9 5,327,564 + Pf1N1B4_4823 0.85 +0.6 5,327,565 - Pf1N1B4_4823 0.85 +0.9 5,327,605 + -1.1 5,327,649 - +1.5 5,327,702 + -1.7 5,327,702 + -0.1 5,327,702 + +0.9 5,327,702 + +0.4 5,327,702 + +0.4 5,327,702 + +0.2 5,327,703 - -0.3 5,327,728 + -0.1 5,327,729 - +0.9 5,327,729 - +1.3 5,327,756 + -2.3 5,327,756 + -0.7 5,327,757 - -1.7 5,327,887 + +0.0 5,327,888 - -0.5 5,327,888 - +0.0 5,328,229 + -2.3 5,328,284 + Pf1N1B4_4824 0.26 -1.4 5,328,285 - Pf1N1B4_4824 0.26 -2.7 5,328,287 - Pf1N1B4_4824 0.26 -2.3 5,328,319 + Pf1N1B4_4824 0.29 +0.9 5,328,464 - +0.9 5,328,721 + +1.3 5,328,721 + -1.7 5,328,949 + Pf1N1B4_4824 0.77 -0.3 5,328,973 + Pf1N1B4_4824 0.79 -2.3 5,329,208 - +0.1 5,329,211 - +0.0 5,329,234 + +1.7 5,329,318 - +1.3 5,329,348 - -0.7 5,329,361 - Pf1N1B4_4825 0.11 -3.0 5,329,361 - Pf1N1B4_4825 0.11 -2.3 5,329,361 - Pf1N1B4_4825 0.11 +0.1 5,329,383 + Pf1N1B4_4825 0.13 -0.1 5,329,383 + Pf1N1B4_4825 0.13 +0.0 5,329,383 + Pf1N1B4_4825 0.13 -0.4 5,329,384 - Pf1N1B4_4825 0.13 +1.6 5,329,384 - -2.7 5,329,686 + Pf1N1B4_4825 0.41 +0.3 5,329,695 + Pf1N1B4_4825 0.42 -1.3 5,329,696 - Pf1N1B4_4825 0.42 -0.1 5,329,696 - Pf1N1B4_4825 0.42 -1.5 5,329,732 + Pf1N1B4_4825 0.45 -0.7 5,329,773 + -0.1 5,330,172 + Pf1N1B4_4825 0.85 -1.7 5,330,173 - Pf1N1B4_4825 0.86 +0.5 5,330,229 + -0.7
Or see this region's nucleotide sequence