Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4760 and Pf1N1B4_4761 overlap by 4 nucleotides Pf1N1B4_4761 and Pf1N1B4_4762 are separated by 64 nucleotides Pf1N1B4_4762 and Pf1N1B4_4763 are separated by 125 nucleotides Pf1N1B4_4763 and Pf1N1B4_4764 are separated by 21 nucleotides
Pf1N1B4_4760 - FIG00962118: hypothetical protein, at 5,258,075 to 5,258,344
_4760
Pf1N1B4_4761 - hypothetical protein, at 5,258,341 to 5,258,739
_4761
Pf1N1B4_4762 - hypothetical protein, at 5,258,804 to 5,259,070
_4762
Pf1N1B4_4763 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 5,259,196 to 5,260,221
_4763
Pf1N1B4_4764 - 2-ketogluconate transporter, at 5,260,243 to 5,261,529
_4764
Position (kb) 5259
5260
5261 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 5258.226 kb on + strand at 5258.503 kb on - strand, within Pf1N1B4_4761 at 5258.503 kb on - strand, within Pf1N1B4_4761 at 5258.558 kb on + strand, within Pf1N1B4_4761 at 5258.800 kb on - strand at 5258.814 kb on + strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.815 kb on - strand at 5258.818 kb on + strand at 5258.819 kb on - strand at 5258.842 kb on - strand, within Pf1N1B4_4762 at 5258.845 kb on + strand, within Pf1N1B4_4762 at 5258.846 kb on - strand, within Pf1N1B4_4762 at 5258.877 kb on + strand, within Pf1N1B4_4762 at 5258.877 kb on + strand, within Pf1N1B4_4762 at 5258.878 kb on - strand, within Pf1N1B4_4762 at 5258.878 kb on - strand, within Pf1N1B4_4762 at 5258.965 kb on - strand, within Pf1N1B4_4762 at 5259.208 kb on + strand at 5259.313 kb on - strand, within Pf1N1B4_4763 at 5259.702 kb on - strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand, within Pf1N1B4_4763 at 5259.793 kb on + strand at 5259.794 kb on - strand, within Pf1N1B4_4763 at 5259.915 kb on + strand, within Pf1N1B4_4763 at 5259.983 kb on + strand, within Pf1N1B4_4763 at 5259.984 kb on - strand at 5260.199 kb on + strand at 5260.199 kb on + strand at 5260.200 kb on - strand at 5260.235 kb on - strand at 5260.235 kb on - strand at 5260.352 kb on + strand at 5260.353 kb on - strand at 5260.353 kb on - strand at 5260.353 kb on - strand at 5260.401 kb on - strand, within Pf1N1B4_4764 at 5260.401 kb on - strand at 5260.517 kb on + strand, within Pf1N1B4_4764 at 5260.517 kb on + strand, within Pf1N1B4_4764 at 5260.518 kb on - strand, within Pf1N1B4_4764 at 5260.518 kb on - strand, within Pf1N1B4_4764 at 5260.550 kb on + strand, within Pf1N1B4_4764 at 5260.550 kb on + strand, within Pf1N1B4_4764 at 5260.593 kb on - strand at 5260.593 kb on - strand, within Pf1N1B4_4764 at 5260.700 kb on + strand, within Pf1N1B4_4764 at 5260.981 kb on - strand, within Pf1N1B4_4764
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,258,226 + -0.7 5,258,503 - Pf1N1B4_4761 0.41 +0.7 5,258,503 - Pf1N1B4_4761 0.41 -0.5 5,258,558 + Pf1N1B4_4761 0.54 -0.6 5,258,800 - -0.4 5,258,814 + -0.4 5,258,815 - -0.4 5,258,815 - -2.3 5,258,815 - -0.4 5,258,815 - +0.7 5,258,815 - +1.6 5,258,818 + +0.3 5,258,819 - -0.7 5,258,842 - Pf1N1B4_4762 0.14 -0.7 5,258,845 + Pf1N1B4_4762 0.15 +0.3 5,258,846 - Pf1N1B4_4762 0.16 -0.5 5,258,877 + Pf1N1B4_4762 0.27 +1.6 5,258,877 + Pf1N1B4_4762 0.27 -1.3 5,258,878 - Pf1N1B4_4762 0.28 -0.7 5,258,878 - Pf1N1B4_4762 0.28 -0.1 5,258,965 - Pf1N1B4_4762 0.60 +1.0 5,259,208 + +0.9 5,259,313 - Pf1N1B4_4763 0.11 -0.2 5,259,702 - Pf1N1B4_4763 0.49 -1.4 5,259,793 + Pf1N1B4_4763 0.58 -1.4 5,259,793 + Pf1N1B4_4763 0.58 -2.0 5,259,793 + Pf1N1B4_4763 0.58 -3.9 5,259,793 + Pf1N1B4_4763 0.58 -2.7 5,259,793 + -0.7 5,259,794 - Pf1N1B4_4763 0.58 +0.7 5,259,915 + Pf1N1B4_4763 0.70 -1.0 5,259,983 + Pf1N1B4_4763 0.77 +0.3 5,259,984 - +0.9 5,260,199 + -1.4 5,260,199 + -0.3 5,260,200 - +2.3 5,260,235 - +2.1 5,260,235 - -0.7 5,260,352 + -1.7 5,260,353 - -0.2 5,260,353 - +0.0 5,260,353 - -1.7 5,260,401 - Pf1N1B4_4764 0.12 -0.7 5,260,401 - -0.7 5,260,517 + Pf1N1B4_4764 0.21 -0.7 5,260,517 + Pf1N1B4_4764 0.21 -0.7 5,260,518 - Pf1N1B4_4764 0.21 -0.5 5,260,518 - Pf1N1B4_4764 0.21 -1.7 5,260,550 + Pf1N1B4_4764 0.24 -1.7 5,260,550 + Pf1N1B4_4764 0.24 -0.7 5,260,593 - +0.3 5,260,593 - Pf1N1B4_4764 0.27 +0.3 5,260,700 + Pf1N1B4_4764 0.36 -2.3 5,260,981 - Pf1N1B4_4764 0.57 -2.0
Or see this region's nucleotide sequence