Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4698 and Pf1N1B4_4699 are separated by 84 nucleotides Pf1N1B4_4699 and Pf1N1B4_4700 are separated by 591 nucleotides Pf1N1B4_4700 and Pf1N1B4_4701 are separated by 19 nucleotides
Pf1N1B4_4698 - rfbN protein, at 5,191,432 to 5,192,499
_4698
Pf1N1B4_4699 - Multidrug translocase MdfA, at 5,192,584 to 5,193,825
_4699
Pf1N1B4_4700 - FIG00956898: hypothetical protein, at 5,194,417 to 5,194,605
_4700
Pf1N1B4_4701 - Fe2+/Zn2+ uptake regulation proteins, at 5,194,625 to 5,194,996
_4701
Position (kb)
5192
5193
5194 Strain fitness (log2 ratio)
-2
-1
0
1 at 5191.691 kb on + strand, within Pf1N1B4_4698 at 5191.692 kb on - strand, within Pf1N1B4_4698 at 5191.728 kb on + strand, within Pf1N1B4_4698 at 5191.730 kb on + strand, within Pf1N1B4_4698 at 5191.731 kb on - strand at 5191.731 kb on - strand, within Pf1N1B4_4698 at 5191.731 kb on - strand, within Pf1N1B4_4698 at 5191.811 kb on + strand, within Pf1N1B4_4698 at 5192.082 kb on - strand, within Pf1N1B4_4698 at 5192.082 kb on - strand, within Pf1N1B4_4698 at 5192.105 kb on + strand, within Pf1N1B4_4698 at 5192.105 kb on + strand, within Pf1N1B4_4698 at 5192.106 kb on - strand, within Pf1N1B4_4698 at 5192.106 kb on - strand, within Pf1N1B4_4698 at 5192.157 kb on - strand, within Pf1N1B4_4698 at 5192.552 kb on - strand at 5192.573 kb on - strand at 5192.776 kb on - strand, within Pf1N1B4_4699 at 5192.844 kb on - strand, within Pf1N1B4_4699 at 5193.261 kb on - strand, within Pf1N1B4_4699 at 5193.651 kb on - strand, within Pf1N1B4_4699 at 5193.743 kb on + strand at 5193.744 kb on - strand at 5193.802 kb on + strand at 5193.803 kb on - strand at 5193.803 kb on - strand at 5193.804 kb on + strand at 5193.805 kb on - strand at 5193.809 kb on - strand at 5194.049 kb on + strand at 5194.050 kb on - strand at 5194.094 kb on + strand at 5194.116 kb on + strand at 5194.534 kb on + strand, within Pf1N1B4_4700 at 5194.534 kb on + strand, within Pf1N1B4_4700
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,191,691 + Pf1N1B4_4698 0.24 -0.7 5,191,692 - Pf1N1B4_4698 0.24 -1.7 5,191,728 + Pf1N1B4_4698 0.28 -1.7 5,191,730 + Pf1N1B4_4698 0.28 -0.7 5,191,731 - -0.7 5,191,731 - Pf1N1B4_4698 0.28 -1.4 5,191,731 - Pf1N1B4_4698 0.28 -2.3 5,191,811 + Pf1N1B4_4698 0.35 -2.3 5,192,082 - Pf1N1B4_4698 0.61 -0.4 5,192,082 - Pf1N1B4_4698 0.61 +0.3 5,192,105 + Pf1N1B4_4698 0.63 +0.9 5,192,105 + Pf1N1B4_4698 0.63 -0.3 5,192,106 - Pf1N1B4_4698 0.63 -0.3 5,192,106 - Pf1N1B4_4698 0.63 +0.9 5,192,157 - Pf1N1B4_4698 0.68 -1.0 5,192,552 - +1.3 5,192,573 - +0.1 5,192,776 - Pf1N1B4_4699 0.15 -2.2 5,192,844 - Pf1N1B4_4699 0.21 -1.4 5,193,261 - Pf1N1B4_4699 0.55 +0.3 5,193,651 - Pf1N1B4_4699 0.86 +1.6 5,193,743 + -0.1 5,193,744 - -2.5 5,193,802 + +0.6 5,193,803 - -1.0 5,193,803 - -1.3 5,193,804 + +1.6 5,193,805 - -1.3 5,193,809 - -1.0 5,194,049 + +0.8 5,194,050 - -0.4 5,194,094 + +0.6 5,194,116 + -1.3 5,194,534 + Pf1N1B4_4700 0.62 +0.9 5,194,534 + Pf1N1B4_4700 0.62 -0.5
Or see this region's nucleotide sequence