Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4626 and Pf1N1B4_4627 are separated by 115 nucleotides Pf1N1B4_4627 and Pf1N1B4_4628 are separated by 209 nucleotides Pf1N1B4_4628 and Pf1N1B4_4629 are separated by 115 nucleotides Pf1N1B4_4629 and Pf1N1B4_4630 overlap by 4 nucleotides
Pf1N1B4_4626 - 3-carboxymuconate cyclase, at 5,102,679 to 5,103,896
_4626
Pf1N1B4_4627 - FIG00957600: hypothetical protein, at 5,104,012 to 5,104,191
_4627
Pf1N1B4_4628 - hypothetical protein, at 5,104,401 to 5,104,526
_4628
Pf1N1B4_4629 - hypothetical protein, at 5,104,642 to 5,104,854
_4629
Pf1N1B4_4630 - hypothetical protein, at 5,104,851 to 5,105,108
_4630
Position (kb)
5104
5105 Strain fitness (log2 ratio)
-2
-1
0
1 at 5103.094 kb on - strand, within Pf1N1B4_4626 at 5103.094 kb on - strand at 5103.094 kb on - strand at 5103.157 kb on + strand at 5103.158 kb on - strand, within Pf1N1B4_4626 at 5103.262 kb on - strand, within Pf1N1B4_4626 at 5103.262 kb on - strand at 5103.570 kb on - strand, within Pf1N1B4_4626 at 5103.809 kb on + strand at 5103.894 kb on + strand at 5104.124 kb on - strand, within Pf1N1B4_4627 at 5104.136 kb on - strand, within Pf1N1B4_4627 at 5104.196 kb on + strand at 5104.197 kb on - strand at 5104.311 kb on - strand at 5104.389 kb on - strand at 5104.437 kb on + strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.170 kb on - strand at 5105.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 5,103,094 - Pf1N1B4_4626 0.34 -2.0 5,103,094 - -0.7 5,103,094 - +0.3 5,103,157 + -1.7 5,103,158 - Pf1N1B4_4626 0.39 -0.7 5,103,262 - Pf1N1B4_4626 0.48 -1.0 5,103,262 - -0.1 5,103,570 - Pf1N1B4_4626 0.73 +0.1 5,103,809 + -2.3 5,103,894 + +0.3 5,104,124 - Pf1N1B4_4627 0.62 -2.1 5,104,136 - Pf1N1B4_4627 0.69 -2.0 5,104,196 + +1.3 5,104,197 - -0.1 5,104,311 - +0.6 5,104,389 - -0.7 5,104,437 + -1.3 5,105,170 - -1.7 5,105,170 - +0.3 5,105,170 - -1.7 5,105,170 - -0.7 5,105,170 - -1.2 5,105,170 - +0.3 5,105,173 + +0.9
Or see this region's nucleotide sequence