Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4093 and Pf1N1B4_4094 are separated by 225 nucleotides Pf1N1B4_4094 and Pf1N1B4_4095 are separated by 10 nucleotides Pf1N1B4_4095 and Pf1N1B4_4096 are separated by 201 nucleotides
Pf1N1B4_4093 - Alkyl hydroperoxide reductase subunit C-like protein, at 4,542,334 to 4,542,987
_4093
Pf1N1B4_4094 - Uncharacterized conserved protein, at 4,543,213 to 4,543,710
_4094
Pf1N1B4_4095 - Transcriptional regulator, LacI family, at 4,543,721 to 4,544,722
_4095
Pf1N1B4_4096 - Nucleoside-diphosphate-sugar epimerases, at 4,544,924 to 4,546,090
_4096
Position (kb) 4543
4544
4545 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.770 kb on - strand, within Pf1N1B4_4093 at 4542.929 kb on - strand at 4542.958 kb on + strand at 4542.959 kb on - strand at 4543.021 kb on + strand at 4543.167 kb on + strand at 4543.167 kb on + strand at 4543.176 kb on - strand at 4543.176 kb on - strand at 4543.380 kb on - strand, within Pf1N1B4_4094 at 4543.445 kb on - strand, within Pf1N1B4_4094 at 4543.536 kb on - strand, within Pf1N1B4_4094 at 4543.928 kb on - strand, within Pf1N1B4_4095 at 4544.388 kb on + strand, within Pf1N1B4_4095 at 4544.639 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.751 kb on + strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.752 kb on - strand at 4544.834 kb on - strand at 4544.946 kb on + strand at 4544.947 kb on - strand at 4545.113 kb on + strand, within Pf1N1B4_4096 at 4545.205 kb on - strand at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.347 kb on + strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.348 kb on - strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.382 kb on + strand, within Pf1N1B4_4096 at 4545.431 kb on + strand, within Pf1N1B4_4096
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,542,770 - Pf1N1B4_4093 0.67 -0.4 4,542,770 - Pf1N1B4_4093 0.67 +1.2 4,542,929 - -1.4 4,542,958 + +0.2 4,542,959 - -1.4 4,543,021 + -0.4 4,543,167 + -0.4 4,543,167 + -3.0 4,543,176 - -1.6 4,543,176 - +1.2 4,543,380 - Pf1N1B4_4094 0.34 -1.4 4,543,445 - Pf1N1B4_4094 0.47 +1.9 4,543,536 - Pf1N1B4_4094 0.65 -2.3 4,543,928 - Pf1N1B4_4095 0.21 -2.4 4,544,388 + Pf1N1B4_4095 0.67 -0.7 4,544,639 + +1.6 4,544,751 + -1.4 4,544,751 + -0.4 4,544,751 + -0.4 4,544,752 - +1.2 4,544,752 - -0.4 4,544,752 - -0.1 4,544,834 - -0.4 4,544,946 + +1.4 4,544,947 - +0.5 4,545,113 + Pf1N1B4_4096 0.16 +0.6 4,545,205 - -1.4 4,545,347 + Pf1N1B4_4096 0.36 -0.4 4,545,347 + Pf1N1B4_4096 0.36 +0.9 4,545,347 + Pf1N1B4_4096 0.36 +0.6 4,545,348 - Pf1N1B4_4096 0.36 -0.4 4,545,348 - Pf1N1B4_4096 0.36 +0.0 4,545,382 + Pf1N1B4_4096 0.39 +0.6 4,545,382 + Pf1N1B4_4096 0.39 -0.4 4,545,431 + Pf1N1B4_4096 0.43 -1.2
Or see this region's nucleotide sequence