Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4037 and Pf1N1B4_4038 overlap by 4 nucleotides Pf1N1B4_4038 and Pf1N1B4_4039 are separated by 2 nucleotides
Pf1N1B4_4037 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 4,488,385 to 4,489,740
_4037
Pf1N1B4_4038 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 4,489,737 to 4,490,234
_4038
Pf1N1B4_4039 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 4,490,237 to 4,492,021
_4039
Position (kb)
4489
4490
4491 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4488.782 kb on + strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4488.783 kb on - strand, within Pf1N1B4_4037 at 4489.185 kb on - strand at 4489.310 kb on + strand, within Pf1N1B4_4037 at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.741 kb on - strand at 4489.745 kb on - strand at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.011 kb on + strand, within Pf1N1B4_4038 at 4490.012 kb on - strand, within Pf1N1B4_4038 at 4490.273 kb on - strand at 4490.354 kb on - strand at 4490.404 kb on - strand at 4490.404 kb on - strand at 4490.563 kb on - strand, within Pf1N1B4_4039 at 4490.950 kb on - strand, within Pf1N1B4_4039 at 4490.998 kb on - strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.060 kb on + strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039 at 4491.061 kb on - strand, within Pf1N1B4_4039
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,488,782 + Pf1N1B4_4037 0.29 -0.3 4,488,783 - Pf1N1B4_4037 0.29 -1.3 4,488,783 - Pf1N1B4_4037 0.29 -1.5 4,489,185 - +0.7 4,489,310 + Pf1N1B4_4037 0.68 -2.3 4,489,741 - +0.2 4,489,741 - +0.7 4,489,741 - -2.3 4,489,745 - -0.9 4,490,011 + Pf1N1B4_4038 0.55 +0.2 4,490,011 + Pf1N1B4_4038 0.55 -0.3 4,490,012 - Pf1N1B4_4038 0.55 -1.7 4,490,273 - -0.9 4,490,354 - -1.9 4,490,404 - -0.3 4,490,404 - -3.3 4,490,563 - Pf1N1B4_4039 0.18 -0.4 4,490,950 - Pf1N1B4_4039 0.40 -3.2 4,490,998 - Pf1N1B4_4039 0.43 -1.9 4,491,060 + Pf1N1B4_4039 0.46 -1.4 4,491,060 + Pf1N1B4_4039 0.46 -0.9 4,491,060 + Pf1N1B4_4039 0.46 -2.2 4,491,061 - Pf1N1B4_4039 0.46 -1.9 4,491,061 - Pf1N1B4_4039 0.46 -0.8 4,491,061 - Pf1N1B4_4039 0.46 -1.7 4,491,061 - Pf1N1B4_4039 0.46 -0.4
Or see this region's nucleotide sequence