Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_402 and Pf1N1B4_403 are separated by 462 nucleotides Pf1N1B4_403 and Pf1N1B4_404 are separated by 112 nucleotides Pf1N1B4_404 and Pf1N1B4_405 are separated by 135 nucleotides
Pf1N1B4_402 - metal-dependent phosphohydrolase, at 471,295 to 471,951
_402
Pf1N1B4_403 - FIG00965925: hypothetical protein, at 472,414 to 472,668
_403
Pf1N1B4_404 - ParE toxin protein, at 472,781 to 473,161
_404
Pf1N1B4_405 - Carbohydrate kinase, PfkB family, at 473,297 to 474,226
_405
Position (kb) 472
473 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 471.564 kb on - strand, within Pf1N1B4_402 at 471.591 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.755 kb on - strand at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.790 kb on + strand, within Pf1N1B4_402 at 471.949 kb on + strand at 471.949 kb on + strand at 472.025 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.260 kb on + strand at 472.261 kb on - strand at 472.261 kb on - strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.385 kb on + strand at 472.386 kb on - strand at 472.386 kb on - strand at 472.410 kb on - strand at 472.425 kb on + strand at 472.460 kb on + strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.461 kb on - strand, within Pf1N1B4_403 at 472.667 kb on - strand at 472.674 kb on + strand at 472.762 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.805 kb on + strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.806 kb on - strand at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.850 kb on + strand, within Pf1N1B4_404 at 472.851 kb on - strand, within Pf1N1B4_404 at 473.256 kb on - strand at 473.256 kb on - strand at 473.256 kb on - strand at 473.312 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 471,564 - Pf1N1B4_402 0.41 +0.2 471,591 + Pf1N1B4_402 0.45 -0.2 471,724 + -1.2 471,724 + Pf1N1B4_402 0.65 -0.2 471,724 + Pf1N1B4_402 0.65 -0.2 471,724 + Pf1N1B4_402 0.65 +1.4 471,724 + Pf1N1B4_402 0.65 -0.2 471,725 - Pf1N1B4_402 0.65 +1.0 471,725 - Pf1N1B4_402 0.65 -0.2 471,725 - Pf1N1B4_402 0.65 -0.1 471,725 - Pf1N1B4_402 0.65 -0.9 471,725 - Pf1N1B4_402 0.65 +0.6 471,754 + Pf1N1B4_402 0.70 -0.2 471,754 + Pf1N1B4_402 0.70 -0.2 471,754 + Pf1N1B4_402 0.70 +1.8 471,755 - +0.8 471,755 - Pf1N1B4_402 0.70 +1.6 471,755 - Pf1N1B4_402 0.70 -1.8 471,755 - Pf1N1B4_402 0.70 -1.2 471,790 + Pf1N1B4_402 0.75 +1.8 471,949 + -0.2 471,949 + -0.5 472,025 + -0.2 472,260 + -1.0 472,260 + +0.2 472,260 + -0.2 472,260 + -0.2 472,261 - -1.0 472,261 - -0.4 472,385 + -0.6 472,385 + +0.8 472,385 + +3.0 472,385 + -1.8 472,386 - -0.2 472,386 - -1.2 472,410 - -0.2 472,425 + -0.2 472,460 + Pf1N1B4_403 0.18 -0.8 472,461 - Pf1N1B4_403 0.18 -1.2 472,461 - Pf1N1B4_403 0.18 -0.9 472,461 - Pf1N1B4_403 0.18 -2.0 472,667 - +1.6 472,674 + -0.8 472,762 + +1.4 472,805 + -0.2 472,805 + -2.5 472,805 + +0.2 472,806 - +1.6 472,806 - -1.8 472,806 - -1.5 472,850 + Pf1N1B4_404 0.18 -1.2 472,850 + Pf1N1B4_404 0.18 +0.5 472,850 + Pf1N1B4_404 0.18 -0.6 472,851 - Pf1N1B4_404 0.18 +1.4 473,256 - -1.2 473,256 - -0.2 473,256 - -0.3 473,312 - +0.5
Or see this region's nucleotide sequence