Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4028 and Pf1N1B4_4029 are separated by 7 nucleotides Pf1N1B4_4029 and Pf1N1B4_4030 are separated by 39 nucleotides
Pf1N1B4_4028 - NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3), at 4,478,252 to 4,479,715
_4028
Pf1N1B4_4029 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 4,479,723 to 4,481,255
_4029
Pf1N1B4_4030 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 4,481,295 to 4,483,148
_4030
Position (kb) 4479
4480
4481
4482 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4479.228 kb on + strand, within Pf1N1B4_4028 at 4479.228 kb on + strand, within Pf1N1B4_4028 at 4479.229 kb on - strand, within Pf1N1B4_4028 at 4479.435 kb on + strand, within Pf1N1B4_4028 at 4479.489 kb on + strand, within Pf1N1B4_4028 at 4479.490 kb on - strand, within Pf1N1B4_4028 at 4479.830 kb on - strand at 4480.155 kb on - strand, within Pf1N1B4_4029 at 4480.162 kb on + strand, within Pf1N1B4_4029 at 4480.259 kb on - strand, within Pf1N1B4_4029 at 4480.259 kb on - strand, within Pf1N1B4_4029 at 4480.352 kb on - strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.444 kb on + strand, within Pf1N1B4_4029 at 4480.588 kb on + strand, within Pf1N1B4_4029 at 4480.801 kb on + strand, within Pf1N1B4_4029 at 4480.802 kb on - strand, within Pf1N1B4_4029 at 4481.325 kb on + strand at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.861 kb on - strand, within Pf1N1B4_4030 at 4481.954 kb on - strand, within Pf1N1B4_4030 at 4482.168 kb on + strand, within Pf1N1B4_4030 at 4482.215 kb on - strand, within Pf1N1B4_4030
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,479,228 + Pf1N1B4_4028 0.67 -3.5 4,479,228 + Pf1N1B4_4028 0.67 -0.9 4,479,229 - Pf1N1B4_4028 0.67 -2.4 4,479,435 + Pf1N1B4_4028 0.81 -1.4 4,479,489 + Pf1N1B4_4028 0.84 -0.4 4,479,490 - Pf1N1B4_4028 0.85 -1.4 4,479,830 - -0.4 4,480,155 - Pf1N1B4_4029 0.28 +0.2 4,480,162 + Pf1N1B4_4029 0.29 -1.9 4,480,259 - Pf1N1B4_4029 0.35 -1.7 4,480,259 - Pf1N1B4_4029 0.35 -1.4 4,480,352 - Pf1N1B4_4029 0.41 +0.2 4,480,444 + Pf1N1B4_4029 0.47 -1.4 4,480,444 + Pf1N1B4_4029 0.47 -0.4 4,480,588 + Pf1N1B4_4029 0.56 +0.6 4,480,801 + Pf1N1B4_4029 0.70 +2.0 4,480,802 - Pf1N1B4_4029 0.70 -1.4 4,481,325 + -2.5 4,481,861 - Pf1N1B4_4030 0.31 -3.2 4,481,861 - Pf1N1B4_4030 0.31 -2.4 4,481,954 - Pf1N1B4_4030 0.36 +1.2 4,482,168 + Pf1N1B4_4030 0.47 +0.9 4,482,215 - Pf1N1B4_4030 0.50 -0.8
Or see this region's nucleotide sequence