Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_400 and Pf1N1B4_401 are separated by 215 nucleotides Pf1N1B4_401 and Pf1N1B4_402 are separated by 220 nucleotides
Pf1N1B4_400 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 469,384 to 470,376
_400
Pf1N1B4_401 - GCN5-related N-acetyltransferase (EC 2.3.1.57), at 470,592 to 471,074
_401
Pf1N1B4_402 - metal-dependent phosphohydrolase, at 471,295 to 471,951
_402
Position (kb) 470
471
472 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 469.629 kb on - strand, within Pf1N1B4_400 at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.324 kb on + strand at 470.325 kb on - strand at 470.332 kb on + strand at 470.332 kb on + strand at 470.333 kb on - strand at 470.418 kb on + strand at 470.419 kb on - strand at 470.420 kb on + strand at 470.421 kb on - strand at 470.436 kb on + strand at 470.447 kb on - strand at 470.448 kb on + strand at 470.450 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.523 kb on + strand at 470.558 kb on + strand at 470.559 kb on - strand at 470.559 kb on - strand at 470.680 kb on + strand, within Pf1N1B4_401 at 470.681 kb on - strand, within Pf1N1B4_401 at 470.681 kb on - strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.815 kb on + strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.816 kb on - strand, within Pf1N1B4_401 at 470.831 kb on - strand, within Pf1N1B4_401 at 471.107 kb on - strand at 471.107 kb on - strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.109 kb on + strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.110 kb on - strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.155 kb on + strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.156 kb on - strand at 471.161 kb on + strand at 471.161 kb on + strand at 471.181 kb on + strand at 471.181 kb on + strand at 471.181 kb on + strand at 471.182 kb on - strand at 471.217 kb on - strand at 471.363 kb on + strand, within Pf1N1B4_402 at 471.364 kb on - strand, within Pf1N1B4_402 at 471.564 kb on - strand, within Pf1N1B4_402 at 471.591 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.724 kb on + strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.725 kb on - strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.754 kb on + strand, within Pf1N1B4_402 at 471.755 kb on - strand at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.755 kb on - strand, within Pf1N1B4_402 at 471.790 kb on + strand, within Pf1N1B4_402 at 471.949 kb on + strand at 471.949 kb on + strand at 472.025 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 469,629 - Pf1N1B4_400 0.25 -0.2 470,324 + +0.4 470,324 + -1.2 470,324 + +0.8 470,324 + -0.1 470,325 - -0.2 470,332 + -1.2 470,332 + -0.2 470,333 - +0.8 470,418 + -2.5 470,419 - -1.2 470,420 + +2.1 470,421 - -0.2 470,436 + +0.4 470,447 - -1.2 470,448 + -1.4 470,450 + -1.8 470,523 + +0.4 470,523 + -2.2 470,523 + -0.8 470,523 + -0.2 470,523 + -0.3 470,558 + -1.2 470,559 - -0.6 470,559 - +0.8 470,680 + Pf1N1B4_401 0.18 -2.5 470,681 - Pf1N1B4_401 0.18 +0.4 470,681 - Pf1N1B4_401 0.18 -0.8 470,815 + Pf1N1B4_401 0.46 -2.1 470,815 + Pf1N1B4_401 0.46 -1.0 470,815 + Pf1N1B4_401 0.46 +0.4 470,815 + Pf1N1B4_401 0.46 -0.2 470,815 + Pf1N1B4_401 0.46 -0.6 470,816 - Pf1N1B4_401 0.46 -0.9 470,816 - Pf1N1B4_401 0.46 -0.2 470,816 - Pf1N1B4_401 0.46 -1.5 470,816 - Pf1N1B4_401 0.46 +0.0 470,816 - Pf1N1B4_401 0.46 -0.2 470,816 - Pf1N1B4_401 0.46 -0.2 470,831 - Pf1N1B4_401 0.49 -2.5 471,107 - +0.5 471,107 - -1.8 471,109 + -0.2 471,109 + +1.4 471,109 + +1.5 471,109 + +2.1 471,109 + +1.1 471,109 + -1.5 471,109 + +0.4 471,109 + +0.4 471,109 + +1.4 471,109 + -0.6 471,110 - -0.2 471,110 - -0.2 471,110 - +0.4 471,110 - +1.2 471,155 + +0.1 471,155 + +0.5 471,155 + -1.8 471,155 + +0.4 471,155 + -3.0 471,155 + -0.5 471,156 - -1.2 471,156 - -0.7 471,156 - -2.2 471,156 - +0.8 471,161 + +0.5 471,161 + -0.2 471,181 + +0.8 471,181 + -2.2 471,181 + -1.2 471,182 - +1.4 471,217 - +0.4 471,363 + Pf1N1B4_402 0.10 +0.3 471,364 - Pf1N1B4_402 0.11 -0.5 471,564 - Pf1N1B4_402 0.41 +0.2 471,591 + Pf1N1B4_402 0.45 -0.2 471,724 + -1.2 471,724 + Pf1N1B4_402 0.65 -0.2 471,724 + Pf1N1B4_402 0.65 -0.2 471,724 + Pf1N1B4_402 0.65 +1.4 471,724 + Pf1N1B4_402 0.65 -0.2 471,725 - Pf1N1B4_402 0.65 +1.0 471,725 - Pf1N1B4_402 0.65 -0.2 471,725 - Pf1N1B4_402 0.65 -0.1 471,725 - Pf1N1B4_402 0.65 -0.9 471,725 - Pf1N1B4_402 0.65 +0.6 471,754 + Pf1N1B4_402 0.70 -0.2 471,754 + Pf1N1B4_402 0.70 -0.2 471,754 + Pf1N1B4_402 0.70 +1.8 471,755 - +0.8 471,755 - Pf1N1B4_402 0.70 +1.6 471,755 - Pf1N1B4_402 0.70 -1.8 471,755 - Pf1N1B4_402 0.70 -1.2 471,790 + Pf1N1B4_402 0.75 +1.8 471,949 + -0.2 471,949 + -0.5 472,025 + -0.2
Or see this region's nucleotide sequence