Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3784 and Pf1N1B4_3785 are separated by 96 nucleotides Pf1N1B4_3785 and Pf1N1B4_3786 are separated by 153 nucleotides Pf1N1B4_3786 and Pf1N1B4_3787 are separated by 83 nucleotides
Pf1N1B4_3784 - Acetoacetyl-CoA synthetase (EC 6.2.1.16) / Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 4,193,040 to 4,194,878
_3784
Pf1N1B4_3785 - FOG: Ankyrin repeat, at 4,194,975 to 4,195,493
_3785
Pf1N1B4_3786 - FIG00955145: hypothetical protein, at 4,195,647 to 4,196,177
_3786
Pf1N1B4_3787 - FIG00960829: hypothetical protein, at 4,196,261 to 4,196,452
_3787
Position (kb)
4194
4195
4196 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4194.101 kb on + strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.102 kb on - strand, within Pf1N1B4_3784 at 4194.114 kb on + strand, within Pf1N1B4_3784 at 4194.207 kb on + strand, within Pf1N1B4_3784 at 4194.208 kb on - strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.239 kb on + strand, within Pf1N1B4_3784 at 4194.387 kb on + strand, within Pf1N1B4_3784 at 4194.388 kb on - strand, within Pf1N1B4_3784 at 4194.388 kb on - strand, within Pf1N1B4_3784 at 4194.463 kb on - strand, within Pf1N1B4_3784 at 4194.546 kb on + strand, within Pf1N1B4_3784 at 4194.546 kb on + strand, within Pf1N1B4_3784 at 4194.547 kb on - strand, within Pf1N1B4_3784 at 4194.669 kb on + strand, within Pf1N1B4_3784 at 4194.670 kb on - strand, within Pf1N1B4_3784 at 4194.792 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.819 kb on + strand at 4194.820 kb on - strand at 4194.820 kb on - strand at 4194.889 kb on - strand at 4194.940 kb on - strand at 4195.100 kb on + strand, within Pf1N1B4_3785 at 4195.100 kb on + strand, within Pf1N1B4_3785 at 4195.101 kb on - strand, within Pf1N1B4_3785 at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.590 kb on - strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.595 kb on + strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.596 kb on - strand at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.788 kb on + strand, within Pf1N1B4_3786 at 4195.789 kb on - strand, within Pf1N1B4_3786 at 4195.872 kb on + strand at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.872 kb on + strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4195.873 kb on - strand, within Pf1N1B4_3786 at 4196.022 kb on + strand, within Pf1N1B4_3786 at 4196.185 kb on + strand at 4196.193 kb on + strand at 4196.194 kb on - strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.228 kb on + strand at 4196.229 kb on - strand at 4196.238 kb on + strand at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.335 kb on + strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.336 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787 at 4196.377 kb on - strand, within Pf1N1B4_3787
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,194,101 + Pf1N1B4_3784 0.58 -0.3 4,194,102 - Pf1N1B4_3784 0.58 -1.3 4,194,102 - Pf1N1B4_3784 0.58 -1.2 4,194,102 - Pf1N1B4_3784 0.58 -1.1 4,194,102 - Pf1N1B4_3784 0.58 -1.3 4,194,102 - Pf1N1B4_3784 0.58 +0.7 4,194,114 + Pf1N1B4_3784 0.58 -0.8 4,194,207 + Pf1N1B4_3784 0.63 +1.3 4,194,208 - Pf1N1B4_3784 0.64 -2.8 4,194,239 + Pf1N1B4_3784 0.65 -0.7 4,194,239 + Pf1N1B4_3784 0.65 +0.3 4,194,239 + Pf1N1B4_3784 0.65 -0.3 4,194,387 + Pf1N1B4_3784 0.73 +0.0 4,194,388 - Pf1N1B4_3784 0.73 +1.9 4,194,388 - Pf1N1B4_3784 0.73 +0.1 4,194,463 - Pf1N1B4_3784 0.77 +0.1 4,194,546 + Pf1N1B4_3784 0.82 -1.8 4,194,546 + Pf1N1B4_3784 0.82 +0.2 4,194,547 - Pf1N1B4_3784 0.82 -0.7 4,194,669 + Pf1N1B4_3784 0.89 -0.3 4,194,670 - Pf1N1B4_3784 0.89 +0.2 4,194,792 + +0.3 4,194,819 + +0.2 4,194,819 + -0.3 4,194,819 + -1.8 4,194,819 + -0.5 4,194,820 - -3.1 4,194,820 - -0.8 4,194,889 - +1.1 4,194,940 - +0.3 4,195,100 + Pf1N1B4_3785 0.24 -1.5 4,195,100 + Pf1N1B4_3785 0.24 -1.3 4,195,101 - Pf1N1B4_3785 0.24 -0.3 4,195,590 - -0.8 4,195,590 - +0.5 4,195,590 - +0.4 4,195,590 - -1.0 4,195,590 - -3.1 4,195,595 + +1.7 4,195,595 + -0.6 4,195,595 + -1.6 4,195,595 + +0.0 4,195,595 + -0.8 4,195,595 + -1.3 4,195,596 - +0.2 4,195,596 - +0.2 4,195,596 - -0.8 4,195,596 - +2.1 4,195,596 - +0.7 4,195,596 - +0.3 4,195,596 - +0.7 4,195,596 - -1.1 4,195,788 + Pf1N1B4_3786 0.27 -1.3 4,195,788 + Pf1N1B4_3786 0.27 +1.1 4,195,788 + Pf1N1B4_3786 0.27 +2.5 4,195,789 - Pf1N1B4_3786 0.27 -2.3 4,195,872 + -0.3 4,195,872 + Pf1N1B4_3786 0.42 -1.3 4,195,872 + Pf1N1B4_3786 0.42 +1.3 4,195,872 + Pf1N1B4_3786 0.42 -0.3 4,195,873 - Pf1N1B4_3786 0.43 -0.7 4,195,873 - Pf1N1B4_3786 0.43 +1.3 4,195,873 - Pf1N1B4_3786 0.43 -0.3 4,195,873 - Pf1N1B4_3786 0.43 -1.8 4,195,873 - Pf1N1B4_3786 0.43 +0.1 4,195,873 - Pf1N1B4_3786 0.43 -2.3 4,195,873 - Pf1N1B4_3786 0.43 -0.4 4,196,022 + Pf1N1B4_3786 0.71 -0.9 4,196,185 + -1.8 4,196,193 + -1.8 4,196,194 - -1.3 4,196,228 + +2.3 4,196,228 + -0.8 4,196,228 + +0.2 4,196,228 + -1.1 4,196,228 + -0.3 4,196,228 + +0.7 4,196,228 + -2.3 4,196,229 - -0.1 4,196,238 + -0.8 4,196,335 + Pf1N1B4_3787 0.39 +0.7 4,196,335 + Pf1N1B4_3787 0.39 -0.4 4,196,336 - Pf1N1B4_3787 0.39 +1.7 4,196,336 - Pf1N1B4_3787 0.39 -2.1 4,196,336 - Pf1N1B4_3787 0.39 +0.1 4,196,336 - Pf1N1B4_3787 0.39 +1.5 4,196,377 - Pf1N1B4_3787 0.60 -0.3 4,196,377 - Pf1N1B4_3787 0.60 -0.7
Or see this region's nucleotide sequence