Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3663 and Pf1N1B4_3664 are separated by 627 nucleotides Pf1N1B4_3664 and Pf1N1B4_3665 are separated by 112 nucleotides Pf1N1B4_3665 and Pf1N1B4_3666 are separated by 134 nucleotides
Pf1N1B4_3663 - GlcG protein, at 4,068,589 to 4,068,849
_3663
Pf1N1B4_3664 - Transposase and inactivated derivatives, at 4,069,477 to 4,069,698
_3664
Pf1N1B4_3665 - Transcriptional regulator, TetR family, at 4,069,811 to 4,070,416
_3665
Pf1N1B4_3666 - Membrane protein, putative, at 4,070,551 to 4,071,465
_3666
Position (kb)
4069
4070
4071 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4069.096 kb on - strand at 4069.129 kb on - strand at 4069.177 kb on + strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.178 kb on - strand at 4069.202 kb on + strand at 4069.203 kb on - strand at 4069.250 kb on + strand at 4069.251 kb on - strand at 4069.251 kb on - strand at 4069.269 kb on - strand at 4069.303 kb on + strand at 4069.314 kb on + strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.315 kb on - strand at 4069.320 kb on + strand at 4069.320 kb on + strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.321 kb on - strand at 4069.373 kb on + strand at 4069.374 kb on - strand at 4069.374 kb on - strand at 4069.430 kb on + strand at 4069.430 kb on + strand at 4069.431 kb on - strand at 4069.431 kb on - strand at 4069.463 kb on + strand at 4069.463 kb on + strand at 4069.464 kb on - strand at 4069.523 kb on + strand, within Pf1N1B4_3664 at 4069.534 kb on - strand, within Pf1N1B4_3664 at 4069.618 kb on + strand, within Pf1N1B4_3664 at 4069.618 kb on + strand, within Pf1N1B4_3664 at 4069.755 kb on - strand at 4069.779 kb on + strand at 4069.793 kb on + strand at 4069.794 kb on - strand at 4069.809 kb on + strand at 4069.932 kb on + strand, within Pf1N1B4_3665 at 4069.932 kb on + strand, within Pf1N1B4_3665 at 4069.932 kb on + strand, within Pf1N1B4_3665 at 4069.932 kb on + strand, within Pf1N1B4_3665 at 4070.160 kb on + strand, within Pf1N1B4_3665 at 4070.277 kb on + strand at 4070.277 kb on + strand, within Pf1N1B4_3665 at 4070.278 kb on - strand, within Pf1N1B4_3665 at 4070.280 kb on + strand, within Pf1N1B4_3665 at 4070.280 kb on + strand, within Pf1N1B4_3665 at 4070.281 kb on - strand, within Pf1N1B4_3665 at 4070.283 kb on + strand, within Pf1N1B4_3665 at 4070.417 kb on - strand at 4070.458 kb on + strand at 4070.839 kb on + strand, within Pf1N1B4_3666 at 4070.839 kb on + strand, within Pf1N1B4_3666 at 4071.227 kb on - strand at 4071.227 kb on - strand, within Pf1N1B4_3666
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 4,069,096 - +0.8 4,069,129 - +0.4 4,069,177 + -0.2 4,069,178 - -1.0 4,069,178 - +0.8 4,069,178 - +0.1 4,069,178 - -0.8 4,069,178 - -0.2 4,069,178 - -1.8 4,069,178 - -0.0 4,069,178 - +0.8 4,069,202 + -1.4 4,069,203 - +1.1 4,069,250 + -2.2 4,069,251 - -1.5 4,069,251 - -2.5 4,069,269 - +1.6 4,069,303 + -0.0 4,069,314 + -0.2 4,069,315 - -0.8 4,069,315 - -0.5 4,069,315 - -0.6 4,069,320 + -0.2 4,069,320 + +0.3 4,069,321 - +0.5 4,069,321 - +0.0 4,069,321 - -0.2 4,069,321 - +0.4 4,069,321 - -0.8 4,069,373 + -0.2 4,069,374 - -1.0 4,069,374 - -1.2 4,069,430 + -1.2 4,069,430 + -0.6 4,069,431 - +0.8 4,069,431 - -1.6 4,069,463 + +1.8 4,069,463 + +0.4 4,069,464 - -0.8 4,069,523 + Pf1N1B4_3664 0.21 +1.8 4,069,534 - Pf1N1B4_3664 0.26 -0.2 4,069,618 + Pf1N1B4_3664 0.64 -2.8 4,069,618 + Pf1N1B4_3664 0.64 +1.0 4,069,755 - -1.2 4,069,779 + -2.2 4,069,793 + +1.8 4,069,794 - -2.5 4,069,809 + -0.8 4,069,932 + Pf1N1B4_3665 0.20 -1.2 4,069,932 + Pf1N1B4_3665 0.20 +0.4 4,069,932 + Pf1N1B4_3665 0.20 -1.2 4,069,932 + Pf1N1B4_3665 0.20 -0.6 4,070,160 + Pf1N1B4_3665 0.58 -1.8 4,070,277 + -0.2 4,070,277 + Pf1N1B4_3665 0.77 -2.2 4,070,278 - Pf1N1B4_3665 0.77 -1.2 4,070,280 + Pf1N1B4_3665 0.77 -0.2 4,070,280 + Pf1N1B4_3665 0.77 -1.2 4,070,281 - Pf1N1B4_3665 0.78 -0.2 4,070,283 + Pf1N1B4_3665 0.78 -1.8 4,070,417 - -1.2 4,070,458 + -1.0 4,070,839 + Pf1N1B4_3666 0.31 +1.1 4,070,839 + Pf1N1B4_3666 0.31 -1.2 4,071,227 - -1.8 4,071,227 - Pf1N1B4_3666 0.74 +1.4
Or see this region's nucleotide sequence