Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_338 and Pf1N1B4_339 are separated by 359 nucleotides Pf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotides Pf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotides
Pf1N1B4_338 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 401,787 to 402,794
_338
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036
_340
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Position (kb) 403
404
405 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 402.198 kb on + strand, within Pf1N1B4_338 at 402.443 kb on - strand, within Pf1N1B4_338 at 402.474 kb on - strand at 402.554 kb on - strand, within Pf1N1B4_338 at 402.881 kb on - strand at 402.930 kb on - strand at 403.004 kb on - strand at 403.016 kb on + strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.017 kb on - strand at 403.148 kb on + strand at 403.148 kb on + strand at 403.149 kb on - strand at 403.159 kb on + strand at 403.493 kb on + strand, within Pf1N1B4_339 at 403.765 kb on + strand, within Pf1N1B4_339 at 403.895 kb on + strand, within Pf1N1B4_339 at 404.132 kb on - strand at 404.155 kb on - strand at 404.155 kb on - strand at 404.219 kb on - strand, within Pf1N1B4_340 at 404.383 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.460 kb on - strand, within Pf1N1B4_340 at 404.466 kb on - strand, within Pf1N1B4_340 at 404.555 kb on + strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand at 404.559 kb on + strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.588 kb on + strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.993 kb on + strand at 404.993 kb on + strand at 404.993 kb on + strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 405.029 kb on - strand at 405.029 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 402,198 + Pf1N1B4_338 0.41 +0.2 402,443 - Pf1N1B4_338 0.65 +1.8 402,474 - -0.2 402,554 - Pf1N1B4_338 0.76 +0.5 402,881 - -1.4 402,930 - -0.2 403,004 - -0.7 403,016 + -1.2 403,017 - +0.5 403,017 - -0.2 403,017 - -1.2 403,017 - -1.0 403,148 + -0.2 403,148 + -0.5 403,149 - -1.4 403,159 + +0.1 403,493 + Pf1N1B4_339 0.35 -1.0 403,765 + Pf1N1B4_339 0.63 -0.2 403,895 + Pf1N1B4_339 0.77 -1.2 404,132 - +0.8 404,155 - -0.6 404,155 - -1.8 404,219 - Pf1N1B4_340 0.11 -2.0 404,383 - Pf1N1B4_340 0.29 -0.8 404,427 - Pf1N1B4_340 0.34 +0.5 404,427 - Pf1N1B4_340 0.34 -0.2 404,460 - Pf1N1B4_340 0.38 -0.5 404,466 - Pf1N1B4_340 0.38 -1.2 404,555 + Pf1N1B4_340 0.48 +1.0 404,556 - Pf1N1B4_340 0.48 +0.4 404,556 - Pf1N1B4_340 0.48 -2.5 404,556 - Pf1N1B4_340 0.48 -2.2 404,556 - Pf1N1B4_340 0.48 -3.0 404,556 - Pf1N1B4_340 0.48 -2.4 404,556 - Pf1N1B4_340 0.48 +0.4 404,556 - Pf1N1B4_340 0.48 -1.2 404,556 - -1.2 404,559 + Pf1N1B4_340 0.48 -2.8 404,560 - Pf1N1B4_340 0.48 +0.6 404,560 - Pf1N1B4_340 0.48 +0.2 404,560 - Pf1N1B4_340 0.48 -0.5 404,560 - Pf1N1B4_340 0.48 -0.2 404,560 - Pf1N1B4_340 0.48 -1.0 404,588 + Pf1N1B4_340 0.51 -1.2 404,589 - Pf1N1B4_340 0.52 -0.2 404,589 - Pf1N1B4_340 0.52 +1.8 404,589 - Pf1N1B4_340 0.52 -0.2 404,669 + Pf1N1B4_340 0.60 -1.4 404,669 + Pf1N1B4_340 0.60 -0.6 404,921 + Pf1N1B4_340 0.87 -0.2 404,921 + Pf1N1B4_340 0.87 +0.5 404,922 - Pf1N1B4_340 0.88 -2.2 404,922 - Pf1N1B4_340 0.88 +0.8 404,922 - Pf1N1B4_340 0.88 -0.6 404,922 - Pf1N1B4_340 0.88 -1.0 404,922 - Pf1N1B4_340 0.88 -0.0 404,922 - Pf1N1B4_340 0.88 -1.2 404,993 + -0.2 404,993 + -1.2 404,993 + +1.8 404,994 - -0.2 404,994 - -0.5 404,994 - -0.8 404,994 - -0.6 405,029 - +0.8 405,029 - -0.5
Or see this region's nucleotide sequence