Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2942 and Pf1N1B4_2943 are separated by 237 nucleotides Pf1N1B4_2943 and Pf1N1B4_2944 are separated by 328 nucleotides Pf1N1B4_2944 and Pf1N1B4_2945 are separated by 193 nucleotides Pf1N1B4_2945 and Pf1N1B4_2946 are separated by 218 nucleotides
Pf1N1B4_2942 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,298,025 to 3,298,609
_2942
Pf1N1B4_2943 - Tryptophan synthase beta chain like (EC 4.2.1.20), at 3,298,847 to 3,299,224
_2943
Pf1N1B4_2944 - FIG00955938: hypothetical protein, at 3,299,553 to 3,299,759
_2944
Pf1N1B4_2945 - FIG00955916: hypothetical protein, at 3,299,953 to 3,300,231
_2945
Pf1N1B4_2946 - Glycerol-3-phosphate O-acyltransferase, at 3,300,450 to 3,301,616
_2946
Position (kb)
3299
3300 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3298.626 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.738 kb on + strand at 3298.739 kb on - strand at 3298.739 kb on - strand at 3298.739 kb on - strand at 3298.803 kb on + strand at 3298.803 kb on + strand at 3298.803 kb on + strand at 3298.803 kb on + strand at 3298.803 kb on + strand at 3298.804 kb on - strand at 3298.804 kb on - strand at 3298.804 kb on - strand at 3298.804 kb on - strand at 3298.839 kb on - strand at 3299.062 kb on + strand, within Pf1N1B4_2943 at 3299.064 kb on + strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.065 kb on - strand, within Pf1N1B4_2943 at 3299.119 kb on - strand, within Pf1N1B4_2943 at 3299.248 kb on + strand at 3299.248 kb on + strand at 3299.249 kb on - strand at 3299.249 kb on - strand at 3299.339 kb on + strand at 3299.360 kb on + strand at 3299.361 kb on - strand at 3299.361 kb on - strand at 3299.374 kb on - strand at 3299.406 kb on + strand at 3299.476 kb on + strand at 3299.545 kb on + strand at 3299.545 kb on + strand at 3299.546 kb on - strand at 3299.546 kb on - strand at 3299.569 kb on + strand at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.647 kb on + strand, within Pf1N1B4_2944 at 3299.648 kb on - strand, within Pf1N1B4_2944 at 3299.648 kb on - strand, within Pf1N1B4_2944 at 3299.854 kb on - strand at 3299.893 kb on - strand at 3299.928 kb on + strand at 3299.928 kb on + strand at 3299.929 kb on - strand at 3299.929 kb on - strand at 3299.929 kb on - strand at 3299.979 kb on + strand at 3299.980 kb on - strand at 3300.309 kb on + strand at 3300.309 kb on + strand at 3300.339 kb on + strand at 3300.339 kb on + strand at 3300.339 kb on + strand at 3300.339 kb on + strand at 3300.340 kb on - strand at 3300.370 kb on + strand at 3300.370 kb on + strand at 3300.370 kb on + strand at 3300.370 kb on + strand at 3300.370 kb on + strand at 3300.370 kb on + strand at 3300.371 kb on - strand at 3300.371 kb on - strand at 3300.371 kb on - strand at 3300.371 kb on - strand at 3300.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 3,298,626 + -0.5 3,298,738 + +0.1 3,298,738 + -1.2 3,298,738 + -0.7 3,298,738 + -0.5 3,298,738 + -0.6 3,298,738 + +0.9 3,298,738 + -0.3 3,298,738 + +1.1 3,298,738 + +0.5 3,298,739 - +0.1 3,298,739 - +0.1 3,298,739 - +1.7 3,298,803 + -0.3 3,298,803 + -0.1 3,298,803 + +0.1 3,298,803 + +0.1 3,298,803 + -1.0 3,298,804 - +0.1 3,298,804 - -0.1 3,298,804 - +1.9 3,298,804 - -0.1 3,298,839 - -0.9 3,299,062 + Pf1N1B4_2943 0.57 -0.5 3,299,064 + Pf1N1B4_2943 0.57 -1.5 3,299,065 - Pf1N1B4_2943 0.58 -0.9 3,299,065 - Pf1N1B4_2943 0.58 -0.6 3,299,065 - Pf1N1B4_2943 0.58 -2.2 3,299,065 - Pf1N1B4_2943 0.58 +0.8 3,299,065 - Pf1N1B4_2943 0.58 +1.1 3,299,065 - Pf1N1B4_2943 0.58 +0.1 3,299,065 - Pf1N1B4_2943 0.58 -2.2 3,299,065 - +1.1 3,299,065 - Pf1N1B4_2943 0.58 +1.7 3,299,065 - Pf1N1B4_2943 0.58 -1.9 3,299,065 - Pf1N1B4_2943 0.58 +2.1 3,299,119 - Pf1N1B4_2943 0.72 +0.7 3,299,248 + -0.5 3,299,248 + -2.2 3,299,249 - -1.9 3,299,249 - +0.1 3,299,339 + +0.1 3,299,360 + +2.1 3,299,361 - +0.1 3,299,361 - -0.2 3,299,374 - -1.5 3,299,406 + +0.1 3,299,476 + +0.1 3,299,545 + -0.3 3,299,545 + -1.5 3,299,546 - -0.3 3,299,546 - -0.2 3,299,569 + -0.2 3,299,647 + Pf1N1B4_2944 0.45 -0.6 3,299,647 + Pf1N1B4_2944 0.45 -2.5 3,299,647 + Pf1N1B4_2944 0.45 -0.5 3,299,647 + Pf1N1B4_2944 0.45 -0.3 3,299,647 + Pf1N1B4_2944 0.45 -1.9 3,299,647 + Pf1N1B4_2944 0.45 +0.1 3,299,647 + Pf1N1B4_2944 0.45 -1.6 3,299,648 - Pf1N1B4_2944 0.46 -0.9 3,299,648 - Pf1N1B4_2944 0.46 -0.6 3,299,854 - +1.5 3,299,893 - +1.3 3,299,928 + -0.9 3,299,928 + -0.7 3,299,929 - +0.1 3,299,929 - +0.8 3,299,929 - +0.8 3,299,979 + -1.5 3,299,980 - +0.1 3,300,309 + +1.4 3,300,309 + +0.2 3,300,339 + -1.2 3,300,339 + +0.8 3,300,339 + +0.1 3,300,339 + +0.1 3,300,340 - -1.5 3,300,370 + +0.1 3,300,370 + +0.1 3,300,370 + +0.5 3,300,370 + -1.5 3,300,370 + -1.2 3,300,370 + -0.6 3,300,371 - -2.5 3,300,371 - -0.6 3,300,371 - +0.1 3,300,371 - +0.2 3,300,452 - +0.6
Or see this region's nucleotide sequence