Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5208 and Pf1N1B4_2884 are separated by 222 nucleotides Pf1N1B4_2884 and Pf1N1B4_2885 overlap by 14 nucleotides Pf1N1B4_2885 and Pf1N1B4_2886 overlap by 8 nucleotides
Pf1N1B4_5208 - 5S RNA, at 3,236,118 to 3,236,237
_5208
Pf1N1B4_2884 - Paraquat-inducible protein A, at 3,236,460 to 3,237,044
_2884
Pf1N1B4_2885 - Paraquat-inducible protein A, at 3,237,031 to 3,237,654
_2885
Pf1N1B4_2886 - Paraquat-inducible protein B, at 3,237,647 to 3,239,950
_2886
Position (kb)
3237
3238 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3236.314 kb on + strand at 3236.315 kb on - strand at 3236.331 kb on - strand at 3236.349 kb on + strand at 3236.349 kb on + strand at 3236.349 kb on + strand at 3236.349 kb on + strand at 3236.350 kb on - strand at 3236.350 kb on - strand at 3236.350 kb on - strand at 3236.350 kb on - strand at 3236.350 kb on - strand at 3236.354 kb on + strand at 3236.354 kb on + strand at 3236.354 kb on + strand at 3236.354 kb on + strand at 3236.354 kb on + strand at 3236.354 kb on + strand at 3236.355 kb on - strand at 3236.355 kb on - strand at 3236.355 kb on - strand at 3236.355 kb on - strand at 3236.355 kb on - strand at 3236.355 kb on - strand at 3236.385 kb on - strand at 3236.509 kb on + strand at 3236.509 kb on + strand at 3236.520 kb on + strand at 3236.529 kb on + strand, within Pf1N1B4_2884 at 3236.529 kb on + strand, within Pf1N1B4_2884 at 3236.530 kb on - strand, within Pf1N1B4_2884 at 3236.530 kb on - strand, within Pf1N1B4_2884 at 3236.632 kb on + strand, within Pf1N1B4_2884 at 3236.633 kb on - strand, within Pf1N1B4_2884 at 3236.806 kb on - strand, within Pf1N1B4_2884 at 3236.820 kb on + strand, within Pf1N1B4_2884 at 3236.820 kb on + strand, within Pf1N1B4_2884 at 3236.829 kb on + strand, within Pf1N1B4_2884 at 3236.829 kb on + strand, within Pf1N1B4_2884 at 3236.830 kb on - strand, within Pf1N1B4_2884 at 3236.839 kb on + strand, within Pf1N1B4_2884 at 3236.839 kb on + strand, within Pf1N1B4_2884 at 3236.839 kb on + strand, within Pf1N1B4_2884 at 3236.879 kb on - strand, within Pf1N1B4_2884 at 3236.923 kb on + strand, within Pf1N1B4_2884 at 3236.924 kb on - strand, within Pf1N1B4_2884 at 3236.924 kb on - strand, within Pf1N1B4_2884 at 3236.980 kb on + strand, within Pf1N1B4_2884 at 3236.980 kb on + strand, within Pf1N1B4_2884 at 3236.980 kb on + strand, within Pf1N1B4_2884 at 3236.980 kb on + strand, within Pf1N1B4_2884 at 3236.980 kb on + strand, within Pf1N1B4_2884 at 3236.981 kb on - strand, within Pf1N1B4_2884 at 3237.084 kb on + strand at 3237.168 kb on + strand at 3237.169 kb on - strand, within Pf1N1B4_2885 at 3237.169 kb on - strand, within Pf1N1B4_2885 at 3237.169 kb on - strand, within Pf1N1B4_2885 at 3237.182 kb on + strand, within Pf1N1B4_2885 at 3237.183 kb on - strand, within Pf1N1B4_2885 at 3237.202 kb on + strand, within Pf1N1B4_2885 at 3237.202 kb on + strand, within Pf1N1B4_2885 at 3237.202 kb on + strand, within Pf1N1B4_2885 at 3237.203 kb on - strand, within Pf1N1B4_2885 at 3237.203 kb on - strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.270 kb on + strand, within Pf1N1B4_2885 at 3237.271 kb on - strand, within Pf1N1B4_2885 at 3237.436 kb on + strand, within Pf1N1B4_2885 at 3237.437 kb on - strand, within Pf1N1B4_2885 at 3237.478 kb on - strand, within Pf1N1B4_2885 at 3237.478 kb on - strand, within Pf1N1B4_2885 at 3237.486 kb on - strand, within Pf1N1B4_2885 at 3237.697 kb on + strand at 3237.697 kb on + strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.698 kb on - strand at 3237.756 kb on - strand at 3237.834 kb on - strand at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.938 kb on + strand, within Pf1N1B4_2886 at 3237.939 kb on - strand, within Pf1N1B4_2886 at 3237.939 kb on - strand, within Pf1N1B4_2886 at 3237.939 kb on - strand, within Pf1N1B4_2886 at 3237.995 kb on - strand, within Pf1N1B4_2886 at 3238.024 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.028 kb on + strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.029 kb on - strand, within Pf1N1B4_2886 at 3238.179 kb on - strand, within Pf1N1B4_2886 at 3238.179 kb on - strand, within Pf1N1B4_2886 at 3238.179 kb on - strand, within Pf1N1B4_2886 at 3238.179 kb on - strand, within Pf1N1B4_2886 at 3238.179 kb on - strand, within Pf1N1B4_2886 at 3238.211 kb on + strand, within Pf1N1B4_2886 at 3238.211 kb on + strand, within Pf1N1B4_2886 at 3238.211 kb on + strand, within Pf1N1B4_2886 at 3238.211 kb on + strand, within Pf1N1B4_2886 at 3238.211 kb on + strand, within Pf1N1B4_2886 at 3238.212 kb on - strand, within Pf1N1B4_2886 at 3238.212 kb on - strand, within Pf1N1B4_2886 at 3238.212 kb on - strand, within Pf1N1B4_2886 at 3238.212 kb on - strand, within Pf1N1B4_2886 at 3238.269 kb on - strand, within Pf1N1B4_2886 at 3238.269 kb on - strand, within Pf1N1B4_2886 at 3238.354 kb on + strand, within Pf1N1B4_2886 at 3238.354 kb on + strand, within Pf1N1B4_2886 at 3238.354 kb on + strand, within Pf1N1B4_2886 at 3238.457 kb on + strand, within Pf1N1B4_2886 at 3238.458 kb on - strand, within Pf1N1B4_2886 at 3238.550 kb on + strand, within Pf1N1B4_2886 at 3238.550 kb on + strand, within Pf1N1B4_2886 at 3238.551 kb on - strand, within Pf1N1B4_2886 at 3238.551 kb on - strand, within Pf1N1B4_2886 at 3238.551 kb on - strand, within Pf1N1B4_2886 at 3238.551 kb on - strand, within Pf1N1B4_2886 at 3238.649 kb on + strand, within Pf1N1B4_2886 at 3238.649 kb on + strand, within Pf1N1B4_2886 at 3238.649 kb on + strand, within Pf1N1B4_2886 at 3238.650 kb on - strand, within Pf1N1B4_2886 at 3238.650 kb on - strand, within Pf1N1B4_2886 at 3238.650 kb on - strand, within Pf1N1B4_2886
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 3,236,314 + -0.3 3,236,315 - +0.7 3,236,331 - +0.1 3,236,349 + -0.9 3,236,349 + -0.3 3,236,349 + +1.1 3,236,349 + +0.1 3,236,350 - +0.1 3,236,350 - -0.7 3,236,350 - -0.3 3,236,350 - -0.6 3,236,350 - -1.4 3,236,354 + -0.3 3,236,354 + -0.9 3,236,354 + +0.6 3,236,354 + -1.2 3,236,354 + +0.4 3,236,354 + +0.5 3,236,355 - +0.1 3,236,355 - +0.6 3,236,355 - -1.4 3,236,355 - +1.7 3,236,355 - +0.7 3,236,355 - -1.4 3,236,385 - -0.9 3,236,509 + -2.4 3,236,509 + -0.9 3,236,520 + +0.1 3,236,529 + Pf1N1B4_2884 0.12 -2.2 3,236,529 + Pf1N1B4_2884 0.12 -0.9 3,236,530 - Pf1N1B4_2884 0.12 -1.4 3,236,530 - Pf1N1B4_2884 0.12 -0.6 3,236,632 + Pf1N1B4_2884 0.29 -1.9 3,236,633 - Pf1N1B4_2884 0.30 +1.3 3,236,806 - Pf1N1B4_2884 0.59 -1.4 3,236,820 + Pf1N1B4_2884 0.62 -2.2 3,236,820 + Pf1N1B4_2884 0.62 +0.1 3,236,829 + Pf1N1B4_2884 0.63 +0.5 3,236,829 + Pf1N1B4_2884 0.63 +0.1 3,236,830 - Pf1N1B4_2884 0.63 -2.2 3,236,839 + Pf1N1B4_2884 0.65 +0.1 3,236,839 + Pf1N1B4_2884 0.65 -1.1 3,236,839 + Pf1N1B4_2884 0.65 +0.1 3,236,879 - Pf1N1B4_2884 0.72 -1.4 3,236,923 + Pf1N1B4_2884 0.79 +2.1 3,236,924 - Pf1N1B4_2884 0.79 +1.1 3,236,924 - Pf1N1B4_2884 0.79 +0.1 3,236,980 + Pf1N1B4_2884 0.89 -0.9 3,236,980 + Pf1N1B4_2884 0.89 -1.2 3,236,980 + Pf1N1B4_2884 0.89 +0.1 3,236,980 + Pf1N1B4_2884 0.89 -0.9 3,236,980 + Pf1N1B4_2884 0.89 -2.0 3,236,981 - Pf1N1B4_2884 0.89 +0.1 3,237,084 + -1.5 3,237,168 + -0.9 3,237,169 - Pf1N1B4_2885 0.22 -2.5 3,237,169 - Pf1N1B4_2885 0.22 -0.9 3,237,169 - Pf1N1B4_2885 0.22 -1.0 3,237,182 + Pf1N1B4_2885 0.24 +0.1 3,237,183 - Pf1N1B4_2885 0.24 -1.1 3,237,202 + Pf1N1B4_2885 0.27 -2.2 3,237,202 + Pf1N1B4_2885 0.27 -1.9 3,237,202 + Pf1N1B4_2885 0.27 -0.9 3,237,203 - Pf1N1B4_2885 0.28 -0.5 3,237,203 - Pf1N1B4_2885 0.28 -0.9 3,237,270 + Pf1N1B4_2885 0.38 +0.1 3,237,270 + Pf1N1B4_2885 0.38 +0.1 3,237,270 + Pf1N1B4_2885 0.38 -1.1 3,237,270 + Pf1N1B4_2885 0.38 -1.5 3,237,270 + Pf1N1B4_2885 0.38 -1.9 3,237,270 + Pf1N1B4_2885 0.38 -0.9 3,237,271 - Pf1N1B4_2885 0.38 -0.1 3,237,436 + Pf1N1B4_2885 0.65 -3.2 3,237,437 - Pf1N1B4_2885 0.65 +0.1 3,237,478 - Pf1N1B4_2885 0.72 -1.5 3,237,478 - Pf1N1B4_2885 0.72 -1.5 3,237,486 - Pf1N1B4_2885 0.73 -1.9 3,237,697 + -0.9 3,237,697 + +0.1 3,237,698 - -1.5 3,237,698 - -0.9 3,237,698 - -2.2 3,237,698 - -0.6 3,237,698 - -0.5 3,237,698 - +0.1 3,237,698 - +0.1 3,237,756 - -1.5 3,237,834 - -2.5 3,237,938 + Pf1N1B4_2886 0.13 -1.9 3,237,938 + Pf1N1B4_2886 0.13 +1.7 3,237,938 + Pf1N1B4_2886 0.13 -0.9 3,237,938 + Pf1N1B4_2886 0.13 +1.1 3,237,938 + Pf1N1B4_2886 0.13 -1.2 3,237,938 + Pf1N1B4_2886 0.13 +0.9 3,237,938 + Pf1N1B4_2886 0.13 -1.2 3,237,939 - Pf1N1B4_2886 0.13 -1.2 3,237,939 - Pf1N1B4_2886 0.13 -0.5 3,237,939 - Pf1N1B4_2886 0.13 -1.2 3,237,995 - Pf1N1B4_2886 0.15 -1.5 3,238,024 + Pf1N1B4_2886 0.16 -0.9 3,238,028 + -0.9 3,238,028 + Pf1N1B4_2886 0.17 -0.9 3,238,028 + Pf1N1B4_2886 0.17 +0.1 3,238,028 + Pf1N1B4_2886 0.17 -2.2 3,238,028 + Pf1N1B4_2886 0.17 +0.5 3,238,028 + Pf1N1B4_2886 0.17 -0.9 3,238,028 + Pf1N1B4_2886 0.17 -0.9 3,238,028 + Pf1N1B4_2886 0.17 -0.9 3,238,028 + Pf1N1B4_2886 0.17 -2.2 3,238,028 + Pf1N1B4_2886 0.17 -0.9 3,238,028 + Pf1N1B4_2886 0.17 -3.2 3,238,029 - Pf1N1B4_2886 0.17 -0.4 3,238,029 - Pf1N1B4_2886 0.17 +2.5 3,238,029 - Pf1N1B4_2886 0.17 -0.5 3,238,029 - Pf1N1B4_2886 0.17 +0.1 3,238,029 - Pf1N1B4_2886 0.17 +0.9 3,238,029 - Pf1N1B4_2886 0.17 -1.5 3,238,029 - Pf1N1B4_2886 0.17 +1.1 3,238,029 - Pf1N1B4_2886 0.17 -0.7 3,238,029 - Pf1N1B4_2886 0.17 -0.9 3,238,029 - Pf1N1B4_2886 0.17 -1.2 3,238,029 - Pf1N1B4_2886 0.17 +0.1 3,238,029 - Pf1N1B4_2886 0.17 -0.9 3,238,179 - Pf1N1B4_2886 0.23 -2.5 3,238,179 - Pf1N1B4_2886 0.23 +0.1 3,238,179 - Pf1N1B4_2886 0.23 -1.2 3,238,179 - Pf1N1B4_2886 0.23 -1.2 3,238,179 - Pf1N1B4_2886 0.23 +0.1 3,238,211 + Pf1N1B4_2886 0.24 -1.2 3,238,211 + Pf1N1B4_2886 0.24 +0.1 3,238,211 + Pf1N1B4_2886 0.24 +0.1 3,238,211 + Pf1N1B4_2886 0.24 -2.5 3,238,211 + Pf1N1B4_2886 0.24 -0.2 3,238,212 - Pf1N1B4_2886 0.25 -0.6 3,238,212 - Pf1N1B4_2886 0.25 +0.5 3,238,212 - Pf1N1B4_2886 0.25 -1.1 3,238,212 - Pf1N1B4_2886 0.25 -0.9 3,238,269 - Pf1N1B4_2886 0.27 +2.5 3,238,269 - Pf1N1B4_2886 0.27 +0.1 3,238,354 + Pf1N1B4_2886 0.31 +0.1 3,238,354 + Pf1N1B4_2886 0.31 -1.5 3,238,354 + Pf1N1B4_2886 0.31 -1.5 3,238,457 + Pf1N1B4_2886 0.35 -1.5 3,238,458 - Pf1N1B4_2886 0.35 -1.7 3,238,550 + Pf1N1B4_2886 0.39 -0.9 3,238,550 + Pf1N1B4_2886 0.39 -0.5 3,238,551 - Pf1N1B4_2886 0.39 -0.3 3,238,551 - Pf1N1B4_2886 0.39 -0.9 3,238,551 - Pf1N1B4_2886 0.39 -2.2 3,238,551 - Pf1N1B4_2886 0.39 -2.0 3,238,649 + Pf1N1B4_2886 0.43 -3.5 3,238,649 + Pf1N1B4_2886 0.43 +0.7 3,238,649 + Pf1N1B4_2886 0.43 -0.3 3,238,650 - Pf1N1B4_2886 0.44 -0.9 3,238,650 - Pf1N1B4_2886 0.44 -1.9 3,238,650 - Pf1N1B4_2886 0.44 +0.1
Or see this region's nucleotide sequence