Experiment: D-Cycloserine 0.1 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2720 and Pf1N1B4_2721 are separated by 185 nucleotides Pf1N1B4_2721 and Pf1N1B4_2722 are separated by 71 nucleotides Pf1N1B4_2722 and Pf1N1B4_2723 overlap by 6 nucleotides 
        Pf1N1B4_2720 - Acyl-CoA dehydrogenase (EC 1.3.8.7), at 3,058,059 to 3,059,708 
        _2720 
         
        
        Pf1N1B4_2721 - AMP nucleosidase (EC 3.2.2.4), at 3,059,894 to 3,061,357 
        _2721 
         
        
        Pf1N1B4_2722 - Ribosomal large subunit pseudouridine synthase E (EC 4.2.1.70), at 3,061,429 to 3,062,064 
        _2722 
         
        
        Pf1N1B4_2723 - FIG00954142: hypothetical protein, at 3,062,059 to 3,062,268 
        _2723 
         Position (kb) 3059 
3060 
3061 
3062 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 
3 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.220 kb on + strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.221 kb on - strand, within Pf1N1B4_2720 at 3059.287 kb on - strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.377 kb on + strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.378 kb on - strand, within Pf1N1B4_2720 at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.566 kb on + strand at 3059.567 kb on - strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.640 kb on + strand at 3059.641 kb on - strand at 3059.733 kb on + strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.734 kb on - strand at 3059.795 kb on + strand at 3059.796 kb on - strand at 3059.796 kb on - strand at 3059.908 kb on + strand at 3059.999 kb on + strand at 3060.000 kb on - strand at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.072 kb on - strand, within Pf1N1B4_2721 at 3060.110 kb on + strand, within Pf1N1B4_2721 at 3060.111 kb on - strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.137 kb on + strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.138 kb on - strand, within Pf1N1B4_2721 at 3060.176 kb on + strand at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.176 kb on + strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.177 kb on - strand, within Pf1N1B4_2721 at 3060.263 kb on + strand, within Pf1N1B4_2721 at 3060.337 kb on + strand, within Pf1N1B4_2721 at 3060.380 kb on + strand, within Pf1N1B4_2721 at 3060.381 kb on - strand, within Pf1N1B4_2721 at 3060.464 kb on + strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.465 kb on - strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.515 kb on + strand, within Pf1N1B4_2721 at 3060.516 kb on - strand, within Pf1N1B4_2721 at 3060.689 kb on + strand at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.854 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.872 kb on + strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3060.873 kb on - strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.034 kb on + strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.035 kb on - strand, within Pf1N1B4_2721 at 3061.235 kb on + strand at 3061.235 kb on + strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.236 kb on - strand at 3061.390 kb on + strand at 3061.412 kb on + strand at 3061.413 kb on - strand at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.732 kb on + strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.733 kb on - strand, within Pf1N1B4_2722 at 3061.809 kb on + strand, within Pf1N1B4_2722 at 3062.060 kb on + strand at 3062.061 kb on - strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.062 kb on + strand at 3062.063 kb on - strand at 3062.063 kb on - strand at 3062.131 kb on + strand, within Pf1N1B4_2723 at 3062.132 kb on - strand, within Pf1N1B4_2723 at 3062.294 kb on + strand at 3062.294 kb on + strand at 3062.295 kb on - strand at 3062.309 kb on + strand at 3062.309 kb on + strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.310 kb on - strand at 3062.311 kb on + strand at 3062.311 kb on + strand at 3062.312 kb on - strand at 3062.312 kb on - strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction D-Cycloserine 0.1 mg/ml remove 3,059,220 +  Pf1N1B4_2720 0.70  +0.2 3,059,220 +  Pf1N1B4_2720 0.70  -1.7 3,059,220 +  Pf1N1B4_2720 0.70  -0.0 3,059,221 -  Pf1N1B4_2720 0.70  -1.3 3,059,221 -  Pf1N1B4_2720 0.70  +0.2 3,059,287 -  Pf1N1B4_2720 0.74  -1.1 3,059,377 +  Pf1N1B4_2720 0.80  +0.6 3,059,377 +  Pf1N1B4_2720 0.80  -2.6 3,059,377 +  Pf1N1B4_2720 0.80  +0.1 3,059,377 +  Pf1N1B4_2720 0.80  -2.1 3,059,378 -  Pf1N1B4_2720 0.80  +1.2 3,059,378 -  Pf1N1B4_2720 0.80  +0.2 3,059,378 -  Pf1N1B4_2720 0.80  +1.0 3,059,566 +  -0.7 3,059,566 +  +0.4 3,059,566 +  +0.0 3,059,567 -  -0.3 3,059,640 +  +0.2 3,059,640 +  -1.1 3,059,640 +  -0.3 3,059,641 -  +1.2 3,059,733 +  +1.2 3,059,734 -  +0.2 3,059,734 -  -0.3 3,059,734 -  -0.8 3,059,734 -  +2.2 3,059,795 +  +0.2 3,059,796 -  +0.2 3,059,796 -  +0.5 3,059,908 +  -2.1 3,059,999 +  -0.8 3,060,000 -  -0.4 3,060,072 -  Pf1N1B4_2721 0.12  +0.2 3,060,072 -  Pf1N1B4_2721 0.12  -0.8 3,060,072 -  Pf1N1B4_2721 0.12  -1.4 3,060,072 -  Pf1N1B4_2721 0.12  -2.4 3,060,072 -  Pf1N1B4_2721 0.12  -2.7 3,060,072 -  Pf1N1B4_2721 0.12  +0.2 3,060,110 +  Pf1N1B4_2721 0.15  -0.8 3,060,111 -  Pf1N1B4_2721 0.15  -0.8 3,060,137 +  Pf1N1B4_2721 0.17  -3.1 3,060,137 +  Pf1N1B4_2721 0.17  -1.8 3,060,137 +  Pf1N1B4_2721 0.17  -2.6 3,060,137 +  Pf1N1B4_2721 0.17  -2.4 3,060,138 -  Pf1N1B4_2721 0.17  -1.4 3,060,138 -  Pf1N1B4_2721 0.17  -2.8 3,060,138 -  Pf1N1B4_2721 0.17  -3.2 3,060,138 -  Pf1N1B4_2721 0.17  -0.8 3,060,176 +  +0.2 3,060,176 +  Pf1N1B4_2721 0.19  -2.8 3,060,176 +  Pf1N1B4_2721 0.19  +0.2 3,060,176 +  Pf1N1B4_2721 0.19  +0.2 3,060,177 -  Pf1N1B4_2721 0.19  -2.6 3,060,177 -  Pf1N1B4_2721 0.19  -2.1 3,060,263 +  Pf1N1B4_2721 0.25  -1.4 3,060,337 +  Pf1N1B4_2721 0.30  -1.2 3,060,380 +  Pf1N1B4_2721 0.33  -2.4 3,060,381 -  Pf1N1B4_2721 0.33  -1.4 3,060,464 +  Pf1N1B4_2721 0.39  -1.8 3,060,465 -  Pf1N1B4_2721 0.39  -0.8 3,060,465 -  Pf1N1B4_2721 0.39  -3.1 3,060,465 -  Pf1N1B4_2721 0.39  -1.4 3,060,515 +  Pf1N1B4_2721 0.42  -2.4 3,060,515 +  Pf1N1B4_2721 0.42  -0.5 3,060,515 +  Pf1N1B4_2721 0.42  -2.1 3,060,516 -  Pf1N1B4_2721 0.42  -1.8 3,060,689 +  -0.8 3,060,854 +  Pf1N1B4_2721 0.66  -2.1 3,060,854 +  Pf1N1B4_2721 0.66  -1.8 3,060,872 +  Pf1N1B4_2721 0.67  -0.4 3,060,872 +  Pf1N1B4_2721 0.67  +1.2 3,060,872 +  Pf1N1B4_2721 0.67  -0.8 3,060,872 +  Pf1N1B4_2721 0.67  -3.6 3,060,872 +  Pf1N1B4_2721 0.67  -1.4 3,060,872 +  Pf1N1B4_2721 0.67  -1.4 3,060,872 +  Pf1N1B4_2721 0.67  -1.9 3,060,873 -  Pf1N1B4_2721 0.67  -1.8 3,060,873 -  Pf1N1B4_2721 0.67  -0.4 3,060,873 -  Pf1N1B4_2721 0.67  -2.5 3,060,873 -  Pf1N1B4_2721 0.67  -1.4 3,061,034 +  Pf1N1B4_2721 0.78  -0.8 3,061,034 +  Pf1N1B4_2721 0.78  -1.2 3,061,034 +  Pf1N1B4_2721 0.78  -1.2 3,061,034 +  Pf1N1B4_2721 0.78  -1.1 3,061,035 -  Pf1N1B4_2721 0.78  -2.8 3,061,035 -  Pf1N1B4_2721 0.78  -1.4 3,061,035 -  Pf1N1B4_2721 0.78  +0.8 3,061,235 +  -0.8 3,061,235 +  -2.6 3,061,236 -  -2.1 3,061,236 -  -2.3 3,061,236 -  -2.9 3,061,236 -  +0.2 3,061,236 -  -0.5 3,061,390 +  -1.5 3,061,412 +  +2.8 3,061,413 -  -0.6 3,061,732 +  Pf1N1B4_2722 0.48  +0.2 3,061,732 +  Pf1N1B4_2722 0.48  +0.6 3,061,732 +  Pf1N1B4_2722 0.48  -1.2 3,061,732 +  Pf1N1B4_2722 0.48  -0.9 3,061,733 -  Pf1N1B4_2722 0.48  +2.0 3,061,733 -  Pf1N1B4_2722 0.48  -0.5 3,061,733 -  Pf1N1B4_2722 0.48  -0.5 3,061,733 -  Pf1N1B4_2722 0.48  -0.8 3,061,733 -  Pf1N1B4_2722 0.48  -1.6 3,061,733 -  Pf1N1B4_2722 0.48  -0.8 3,061,809 +  Pf1N1B4_2722 0.60  +0.1 3,062,060 +  +0.2 3,062,061 -  -0.8 3,062,062 +  -0.4 3,062,062 +  -1.0 3,062,062 +  +0.6 3,062,062 +  +1.0 3,062,062 +  -0.5 3,062,062 +  -1.6 3,062,063 -  +2.2 3,062,063 -  -0.3 3,062,131 +  Pf1N1B4_2723 0.34  -0.8 3,062,132 -  Pf1N1B4_2723 0.35  -0.1 3,062,294 +  -2.2 3,062,294 +  -0.8 3,062,295 -  +1.2 3,062,309 +  +0.8 3,062,309 +  -2.1 3,062,310 -  -0.8 3,062,310 -  +0.8 3,062,310 -  -1.1 3,062,311 +  +1.0 3,062,311 +  +1.6 3,062,312 -  +3.2 3,062,312 -  -0.8 
Or see this region's nucleotide sequence