Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2675 and Pf1N1B4_2676 are separated by 187 nucleotides Pf1N1B4_2676 and Pf1N1B4_2677 overlap by 4 nucleotides Pf1N1B4_2677 and Pf1N1B4_2678 overlap by 8 nucleotides
Pf1N1B4_2675 - Nitrogen regulation protein NR(I), at 3,012,410 to 3,014,323
_2675
Pf1N1B4_2676 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase (EC 6.3.2.-), at 3,014,511 to 3,015,860
_2676
Pf1N1B4_2677 - 3-polyprenyl-4-hydroxybenzoate carboxy-lyase UbiX (EC 4.1.1.-), at 3,015,857 to 3,016,489
_2677
Pf1N1B4_2678 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 3,016,482 to 3,016,766
_2678
Position (kb)
3014
3015
3016 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3013.698 kb on + strand, within Pf1N1B4_2675 at 3013.699 kb on - strand, within Pf1N1B4_2675 at 3013.699 kb on - strand, within Pf1N1B4_2675 at 3013.699 kb on - strand, within Pf1N1B4_2675 at 3013.699 kb on - strand, within Pf1N1B4_2675 at 3013.746 kb on + strand, within Pf1N1B4_2675 at 3013.749 kb on + strand, within Pf1N1B4_2675 at 3013.749 kb on + strand, within Pf1N1B4_2675 at 3013.749 kb on + strand, within Pf1N1B4_2675 at 3013.750 kb on - strand, within Pf1N1B4_2675 at 3013.750 kb on - strand, within Pf1N1B4_2675 at 3013.750 kb on - strand, within Pf1N1B4_2675 at 3013.750 kb on - strand, within Pf1N1B4_2675 at 3013.880 kb on + strand, within Pf1N1B4_2675 at 3013.880 kb on + strand, within Pf1N1B4_2675 at 3013.880 kb on + strand, within Pf1N1B4_2675 at 3013.880 kb on + strand, within Pf1N1B4_2675 at 3013.880 kb on + strand, within Pf1N1B4_2675 at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3013.881 kb on - strand at 3013.881 kb on - strand, within Pf1N1B4_2675 at 3014.055 kb on + strand, within Pf1N1B4_2675 at 3014.055 kb on + strand, within Pf1N1B4_2675 at 3014.281 kb on + strand at 3014.282 kb on - strand at 3014.288 kb on + strand at 3014.312 kb on - strand at 3014.321 kb on + strand at 3014.498 kb on + strand at 3014.499 kb on - strand at 3014.510 kb on + strand at 3014.510 kb on + strand at 3014.522 kb on + strand at 3014.522 kb on + strand at 3014.522 kb on + strand at 3014.748 kb on + strand, within Pf1N1B4_2676 at 3014.748 kb on + strand, within Pf1N1B4_2676 at 3014.748 kb on + strand, within Pf1N1B4_2676 at 3014.772 kb on + strand, within Pf1N1B4_2676 at 3015.489 kb on + strand, within Pf1N1B4_2676 at 3015.489 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.487 kb on + strand at 3016.578 kb on + strand, within Pf1N1B4_2678 at 3016.578 kb on + strand, within Pf1N1B4_2678 at 3016.603 kb on - strand, within Pf1N1B4_2678 at 3016.738 kb on + strand at 3016.764 kb on + strand at 3016.764 kb on + strand at 3016.764 kb on + strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.765 kb on - strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.810 kb on + strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.811 kb on - strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.832 kb on + strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand at 3016.833 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 3,013,698 + Pf1N1B4_2675 0.67 +2.2 3,013,699 - Pf1N1B4_2675 0.67 +1.0 3,013,699 - Pf1N1B4_2675 0.67 +1.2 3,013,699 - Pf1N1B4_2675 0.67 +0.2 3,013,699 - Pf1N1B4_2675 0.67 -2.1 3,013,746 + Pf1N1B4_2675 0.70 +1.2 3,013,749 + Pf1N1B4_2675 0.70 -1.8 3,013,749 + Pf1N1B4_2675 0.70 +1.4 3,013,749 + Pf1N1B4_2675 0.70 -2.0 3,013,750 - Pf1N1B4_2675 0.70 +0.8 3,013,750 - Pf1N1B4_2675 0.70 -0.8 3,013,750 - Pf1N1B4_2675 0.70 -0.1 3,013,750 - Pf1N1B4_2675 0.70 -0.5 3,013,880 + Pf1N1B4_2675 0.77 +0.5 3,013,880 + Pf1N1B4_2675 0.77 -0.8 3,013,880 + Pf1N1B4_2675 0.77 -2.1 3,013,880 + Pf1N1B4_2675 0.77 +0.7 3,013,880 + Pf1N1B4_2675 0.77 -0.2 3,013,881 - Pf1N1B4_2675 0.77 -1.3 3,013,881 - Pf1N1B4_2675 0.77 -1.8 3,013,881 - Pf1N1B4_2675 0.77 -0.8 3,013,881 - Pf1N1B4_2675 0.77 +1.8 3,013,881 - Pf1N1B4_2675 0.77 +1.2 3,013,881 - +0.2 3,013,881 - Pf1N1B4_2675 0.77 -0.4 3,014,055 + Pf1N1B4_2675 0.86 -1.3 3,014,055 + Pf1N1B4_2675 0.86 -1.2 3,014,281 + +0.4 3,014,282 - +2.6 3,014,288 + -2.6 3,014,312 - +0.2 3,014,321 + -0.9 3,014,498 + -1.1 3,014,499 - +0.2 3,014,510 + +1.0 3,014,510 + -1.6 3,014,522 + +0.2 3,014,522 + +0.8 3,014,522 + -1.3 3,014,748 + Pf1N1B4_2676 0.18 -1.8 3,014,748 + Pf1N1B4_2676 0.18 +0.2 3,014,748 + Pf1N1B4_2676 0.18 +0.2 3,014,772 + Pf1N1B4_2676 0.19 +1.2 3,015,489 + Pf1N1B4_2676 0.72 -0.8 3,015,489 + +1.8 3,016,487 + -0.3 3,016,487 + -0.7 3,016,487 + -1.2 3,016,487 + +0.7 3,016,578 + Pf1N1B4_2678 0.34 -0.2 3,016,578 + Pf1N1B4_2678 0.34 -1.1 3,016,603 - Pf1N1B4_2678 0.42 +0.2 3,016,738 + +0.2 3,016,764 + +1.0 3,016,764 + -0.6 3,016,764 + -0.6 3,016,765 - -1.8 3,016,765 - -0.2 3,016,765 - +1.0 3,016,765 - -1.3 3,016,765 - -0.1 3,016,765 - +0.0 3,016,810 + +1.8 3,016,810 + +1.8 3,016,810 + -0.4 3,016,810 + -1.3 3,016,810 + -0.3 3,016,811 - -1.6 3,016,811 - -0.4 3,016,811 - -0.3 3,016,811 - -0.8 3,016,832 + -0.8 3,016,832 + +0.7 3,016,832 + +2.0 3,016,832 + +0.2 3,016,832 + +0.2 3,016,832 + -0.3 3,016,833 - +0.2 3,016,833 - +0.2 3,016,833 - +0.2 3,016,833 - +1.0 3,016,833 - +0.8 3,016,833 - +0.9 3,016,833 - -0.8
Or see this region's nucleotide sequence