Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_265 and Pf1N1B4_266 are separated by 284 nucleotides Pf1N1B4_266 and Pf1N1B4_267 overlap by 42 nucleotides Pf1N1B4_267 and Pf1N1B4_268 are separated by 117 nucleotides
Pf1N1B4_265 - DEAD-box ATP-dependent RNA helicase CshA (EC 3.6.4.13), at 324,901 to 326,574
_265
Pf1N1B4_266 - hypothetical protein, at 326,859 to 327,038
_266
Pf1N1B4_267 - Enoyl-CoA hydratase (EC 4.2.1.17), at 326,997 to 327,788
_267
Pf1N1B4_268 - Spermidine synthase (EC 2.5.1.16), at 327,906 to 328,661
_268
Position (kb) 326
327
328 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 326.587 kb on - strand at 326.587 kb on - strand at 326.633 kb on - strand at 326.760 kb on - strand at 326.796 kb on - strand at 326.796 kb on - strand at 326.796 kb on - strand at 326.892 kb on - strand, within Pf1N1B4_266 at 326.892 kb on - strand at 326.892 kb on - strand, within Pf1N1B4_266 at 327.022 kb on + strand at 327.123 kb on + strand, within Pf1N1B4_267 at 327.124 kb on - strand at 327.324 kb on + strand, within Pf1N1B4_267 at 327.324 kb on + strand, within Pf1N1B4_267 at 327.494 kb on + strand, within Pf1N1B4_267 at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.795 kb on + strand at 327.796 kb on - strand at 327.907 kb on + strand at 327.907 kb on + strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 327.908 kb on - strand at 328.082 kb on - strand, within Pf1N1B4_268 at 328.231 kb on + strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.232 kb on - strand, within Pf1N1B4_268 at 328.276 kb on - strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.303 kb on + strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.304 kb on - strand, within Pf1N1B4_268 at 328.333 kb on + strand, within Pf1N1B4_268 at 328.334 kb on - strand, within Pf1N1B4_268 at 328.502 kb on - strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.507 kb on + strand, within Pf1N1B4_268 at 328.508 kb on - strand, within Pf1N1B4_268 at 328.623 kb on + strand at 328.623 kb on + strand at 328.624 kb on - strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.696 kb on + strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.697 kb on - strand at 328.699 kb on + strand at 328.699 kb on + strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.700 kb on - strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.765 kb on + strand at 328.766 kb on - strand at 328.766 kb on - strand at 328.775 kb on + strand at 328.780 kb on + strand at 328.781 kb on - strand at 328.781 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 326,587 - -0.5 326,587 - -0.2 326,633 - -0.8 326,760 - -1.0 326,796 - +1.8 326,796 - -0.6 326,796 - -0.6 326,892 - Pf1N1B4_266 0.18 +0.8 326,892 - -0.2 326,892 - Pf1N1B4_266 0.18 -0.8 327,022 + +1.4 327,123 + Pf1N1B4_267 0.16 -3.4 327,124 - -0.2 327,324 + Pf1N1B4_267 0.41 -0.2 327,324 + Pf1N1B4_267 0.41 -1.8 327,494 + Pf1N1B4_267 0.63 -1.6 327,795 + -0.8 327,795 + -1.2 327,795 + -0.2 327,795 + +0.3 327,795 + +1.2 327,795 + +0.3 327,795 + -0.2 327,795 + +1.4 327,795 + -1.5 327,796 - -1.0 327,907 + -1.8 327,907 + +0.6 327,908 - +0.4 327,908 - -0.0 327,908 - -0.5 327,908 - +1.8 327,908 - -1.0 327,908 - -0.6 328,082 - Pf1N1B4_268 0.23 -0.2 328,231 + Pf1N1B4_268 0.43 +0.6 328,232 - Pf1N1B4_268 0.43 +1.8 328,232 - Pf1N1B4_268 0.43 +0.8 328,276 - Pf1N1B4_268 0.49 -0.2 328,303 + Pf1N1B4_268 0.53 -0.9 328,303 + Pf1N1B4_268 0.53 -1.8 328,304 - Pf1N1B4_268 0.53 +1.1 328,304 - Pf1N1B4_268 0.53 -0.6 328,304 - Pf1N1B4_268 0.53 +0.2 328,304 - Pf1N1B4_268 0.53 +0.6 328,333 + Pf1N1B4_268 0.56 -0.0 328,334 - Pf1N1B4_268 0.57 -1.6 328,502 - Pf1N1B4_268 0.79 -0.8 328,507 + Pf1N1B4_268 0.79 -0.0 328,507 + Pf1N1B4_268 0.79 -1.8 328,508 - Pf1N1B4_268 0.80 -1.0 328,623 + +1.8 328,623 + -0.8 328,624 - -0.1 328,696 + -0.2 328,696 + -1.2 328,696 + -1.2 328,696 + +0.7 328,696 + +0.1 328,697 - +0.4 328,697 - +0.5 328,697 - +0.8 328,697 - -2.2 328,697 - +0.9 328,697 - +0.2 328,697 - +1.4 328,697 - -0.8 328,697 - -0.4 328,699 + +0.5 328,699 + -1.2 328,700 - -0.6 328,700 - +0.0 328,700 - +1.8 328,700 - +0.3 328,700 - +1.4 328,700 - -0.0 328,700 - +0.8 328,700 - -0.5 328,765 + -1.8 328,765 + -1.2 328,765 + +2.1 328,766 - +0.6 328,766 - -0.5 328,775 + +0.6 328,780 + +0.1 328,781 - -1.1 328,781 - -1.0
Or see this region's nucleotide sequence