Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_254 and Pf1N1B4_255 are separated by 0 nucleotides Pf1N1B4_255 and Pf1N1B4_256 overlap by 4 nucleotides Pf1N1B4_256 and Pf1N1B4_257 are separated by 111 nucleotides
Pf1N1B4_254 - Adenylate cyclase (EC 4.6.1.1), at 312,051 to 314,996
_254
Pf1N1B4_255 - Free methionine-(R)-sulfoxide reductase, contains GAF domain, at 314,997 to 315,479
_255
Pf1N1B4_256 - putative ATP/GTP-binding protein, at 315,476 to 316,366
_256
Pf1N1B4_257 - sensor histidine kinase/response regulator, at 316,478 to 318,841
_257
Position (kb) 314
315
316 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 314.046 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.145 kb on + strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.146 kb on - strand, within Pf1N1B4_254 at 314.194 kb on + strand, within Pf1N1B4_254 at 314.195 kb on - strand, within Pf1N1B4_254 at 314.195 kb on - strand, within Pf1N1B4_254 at 314.509 kb on - strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand, within Pf1N1B4_254 at 314.514 kb on + strand at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand, within Pf1N1B4_254 at 314.515 kb on - strand at 314.532 kb on + strand, within Pf1N1B4_254 at 314.532 kb on + strand, within Pf1N1B4_254 at 314.533 kb on - strand, within Pf1N1B4_254 at 314.533 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand at 314.578 kb on - strand, within Pf1N1B4_254 at 314.578 kb on - strand, within Pf1N1B4_254 at 314.608 kb on + strand at 314.648 kb on + strand, within Pf1N1B4_254 at 314.648 kb on + strand, within Pf1N1B4_254 at 314.796 kb on + strand at 314.796 kb on + strand at 314.796 kb on + strand at 314.797 kb on - strand at 314.904 kb on + strand at 314.904 kb on + strand at 314.905 kb on - strand at 314.914 kb on - strand at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand, within Pf1N1B4_255 at 315.186 kb on + strand at 315.186 kb on + strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand, within Pf1N1B4_255 at 315.187 kb on - strand at 315.187 kb on - strand, within Pf1N1B4_255 at 315.387 kb on - strand, within Pf1N1B4_255 at 315.468 kb on + strand at 315.469 kb on - strand at 315.529 kb on - strand at 315.685 kb on - strand, within Pf1N1B4_256 at 315.685 kb on - strand, within Pf1N1B4_256 at 315.685 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.832 kb on - strand, within Pf1N1B4_256 at 315.844 kb on + strand, within Pf1N1B4_256 at 315.844 kb on + strand, within Pf1N1B4_256 at 316.392 kb on + strand at 316.392 kb on + strand at 316.392 kb on + strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.393 kb on - strand at 316.427 kb on - strand at 316.434 kb on + strand at 316.434 kb on + strand at 316.435 kb on - strand at 316.435 kb on - strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.477 kb on + strand at 316.478 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 314,046 + Pf1N1B4_254 0.68 -1.0 314,145 + Pf1N1B4_254 0.71 -0.6 314,145 + Pf1N1B4_254 0.71 -1.8 314,145 + Pf1N1B4_254 0.71 -1.8 314,145 + Pf1N1B4_254 0.71 +0.1 314,146 - Pf1N1B4_254 0.71 -1.2 314,146 - Pf1N1B4_254 0.71 +1.8 314,146 - Pf1N1B4_254 0.71 -0.2 314,146 - Pf1N1B4_254 0.71 -0.2 314,146 - Pf1N1B4_254 0.71 -1.2 314,194 + Pf1N1B4_254 0.73 -0.2 314,195 - Pf1N1B4_254 0.73 +0.2 314,195 - Pf1N1B4_254 0.73 -0.1 314,509 - Pf1N1B4_254 0.83 -0.1 314,514 + Pf1N1B4_254 0.84 -0.2 314,514 + Pf1N1B4_254 0.84 -2.5 314,514 + Pf1N1B4_254 0.84 -0.6 314,514 + Pf1N1B4_254 0.84 +0.8 314,514 + -0.8 314,515 - Pf1N1B4_254 0.84 +2.1 314,515 - Pf1N1B4_254 0.84 +1.2 314,515 - Pf1N1B4_254 0.84 -0.5 314,515 - -0.2 314,532 + Pf1N1B4_254 0.84 -0.2 314,532 + Pf1N1B4_254 0.84 -0.5 314,533 - Pf1N1B4_254 0.84 +1.4 314,533 - Pf1N1B4_254 0.84 -1.4 314,578 - Pf1N1B4_254 0.86 -1.2 314,578 - -0.2 314,578 - Pf1N1B4_254 0.86 +0.3 314,578 - Pf1N1B4_254 0.86 +0.2 314,608 + -0.2 314,648 + Pf1N1B4_254 0.88 -0.5 314,648 + Pf1N1B4_254 0.88 +0.2 314,796 + -0.6 314,796 + -0.2 314,796 + -0.1 314,797 - +0.9 314,904 + +0.8 314,904 + -0.6 314,905 - -0.4 314,914 - -1.0 315,186 + Pf1N1B4_255 0.39 -0.2 315,186 + Pf1N1B4_255 0.39 -1.2 315,186 + Pf1N1B4_255 0.39 -3.0 315,186 + Pf1N1B4_255 0.39 -3.2 315,186 + -0.2 315,186 + Pf1N1B4_255 0.39 -0.8 315,187 - Pf1N1B4_255 0.39 -0.4 315,187 - Pf1N1B4_255 0.39 -0.8 315,187 - Pf1N1B4_255 0.39 +0.8 315,187 - Pf1N1B4_255 0.39 -1.5 315,187 - Pf1N1B4_255 0.39 -0.9 315,187 - Pf1N1B4_255 0.39 -1.0 315,187 - -0.2 315,187 - Pf1N1B4_255 0.39 -0.8 315,387 - Pf1N1B4_255 0.81 -0.2 315,468 + -1.0 315,469 - -2.8 315,529 - +0.6 315,685 - Pf1N1B4_256 0.23 -0.2 315,685 - Pf1N1B4_256 0.23 -0.8 315,685 - Pf1N1B4_256 0.23 -0.4 315,832 - Pf1N1B4_256 0.40 -0.2 315,832 - Pf1N1B4_256 0.40 +0.1 315,832 - Pf1N1B4_256 0.40 -0.6 315,844 + Pf1N1B4_256 0.41 -2.2 315,844 + Pf1N1B4_256 0.41 -0.0 316,392 + -0.7 316,392 + +0.6 316,392 + -0.4 316,393 - +1.9 316,393 - +0.8 316,393 - -0.2 316,393 - -1.2 316,393 - -1.0 316,393 - -0.8 316,427 - +0.8 316,434 + -0.2 316,434 + -0.8 316,435 - +2.8 316,435 - +0.5 316,477 + -0.8 316,477 + +0.8 316,477 + +1.4 316,477 + +1.4 316,478 - -1.5
Or see this region's nucleotide sequence