Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2386

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2385 and Pf1N1B4_2386 are separated by 325 nucleotidesPf1N1B4_2386 and Pf1N1B4_2387 are separated by 205 nucleotides Pf1N1B4_2385 - Adenosylhomocysteinase (EC 3.3.1.1), at 2,702,566 to 2,703,975 _2385 Pf1N1B4_2386 - cytosolic long-chain acyl-CoA thioester hydrolase family protein, at 2,704,301 to 2,704,705 _2386 Pf1N1B4_2387 - Putative transport, at 2,704,911 to 2,705,798 _2387 Position (kb) 2704 2705Strain fitness (log2 ratio) -2 -1 0 1 2at 2703.993 kb on - strandat 2703.993 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2703.998 kb on - strandat 2704.155 kb on - strandat 2704.165 kb on - strandat 2704.210 kb on + strandat 2704.222 kb on + strandat 2704.223 kb on - strandat 2704.237 kb on - strandat 2704.244 kb on + strandat 2704.277 kb on + strandat 2704.278 kb on - strandat 2704.300 kb on + strandat 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.406 kb on + strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.407 kb on - strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.448 kb on + strand, within Pf1N1B4_2386at 2704.449 kb on - strand, within Pf1N1B4_2386at 2704.449 kb on - strand, within Pf1N1B4_2386at 2704.588 kb on + strand, within Pf1N1B4_2386at 2704.664 kb on + strand, within Pf1N1B4_2386at 2704.664 kb on + strand, within Pf1N1B4_2386at 2704.840 kb on + strandat 2704.877 kb on + strandat 2704.878 kb on - strandat 2704.882 kb on - strandat 2704.910 kb on + strandat 2704.910 kb on + strandat 2704.910 kb on + strandat 2704.911 kb on - strandat 2704.911 kb on - strandat 2705.027 kb on + strandat 2705.028 kb on - strand, within Pf1N1B4_2387at 2705.245 kb on + strand, within Pf1N1B4_2387at 2705.246 kb on - strand, within Pf1N1B4_2387at 2705.267 kb on + strand, within Pf1N1B4_2387at 2705.268 kb on - strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.349 kb on + strand, within Pf1N1B4_2387at 2705.350 kb on - strand, within Pf1N1B4_2387at 2705.350 kb on - strand, within Pf1N1B4_2387at 2705.350 kb on - strand, within Pf1N1B4_2387at 2705.350 kb on - strand, within Pf1N1B4_2387at 2705.544 kb on + strand, within Pf1N1B4_2387at 2705.544 kb on + strand, within Pf1N1B4_2387at 2705.544 kb on + strand, within Pf1N1B4_2387at 2705.545 kb on - strand, within Pf1N1B4_2387at 2705.545 kb on - strand, within Pf1N1B4_2387at 2705.545 kb on - strand, within Pf1N1B4_2387at 2705.601 kb on + strand, within Pf1N1B4_2387at 2705.601 kb on + strand, within Pf1N1B4_2387at 2705.601 kb on + strand, within Pf1N1B4_2387at 2705.602 kb on - strand, within Pf1N1B4_2387at 2705.602 kb on - strand, within Pf1N1B4_2387at 2705.647 kb on + strand, within Pf1N1B4_2387at 2705.647 kb on + strand, within Pf1N1B4_2387at 2705.648 kb on - strand, within Pf1N1B4_2387at 2705.693 kb on + strand, within Pf1N1B4_2387at 2705.693 kb on + strand, within Pf1N1B4_2387at 2705.693 kb on + strand, within Pf1N1B4_2387at 2705.694 kb on - strand, within Pf1N1B4_2387

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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2,703,993 - -0.0
2,703,993 - -0.8
2,703,998 - -0.3
2,703,998 - -1.3
2,703,998 - +1.3
2,703,998 - -0.7
2,704,155 - +0.9
2,704,165 - +0.3
2,704,210 + -0.7
2,704,222 + +1.9
2,704,223 - +2.3
2,704,237 - -0.5
2,704,244 + +1.3
2,704,277 + +0.0
2,704,278 - +0.3
2,704,300 + +0.3
2,704,406 + Pf1N1B4_2386 0.26 +0.7
2,704,406 + Pf1N1B4_2386 0.26 -2.5
2,704,406 + Pf1N1B4_2386 0.26 -2.3
2,704,406 + Pf1N1B4_2386 0.26 -0.1
2,704,406 + Pf1N1B4_2386 0.26 -0.7
2,704,406 + Pf1N1B4_2386 0.26 -2.0
2,704,406 + Pf1N1B4_2386 0.26 -1.7
2,704,407 - Pf1N1B4_2386 0.26 -1.3
2,704,407 - Pf1N1B4_2386 0.26 +0.3
2,704,407 - Pf1N1B4_2386 0.26 -1.8
2,704,407 - Pf1N1B4_2386 0.26 -2.3
2,704,448 + Pf1N1B4_2386 0.36 -1.3
2,704,448 + Pf1N1B4_2386 0.36 +0.3
2,704,448 + Pf1N1B4_2386 0.36 -0.3
2,704,449 - Pf1N1B4_2386 0.37 +0.3
2,704,449 - Pf1N1B4_2386 0.37 -1.7
2,704,588 + Pf1N1B4_2386 0.71 -2.0
2,704,664 + Pf1N1B4_2386 0.90 +0.3
2,704,664 + Pf1N1B4_2386 0.90 +0.3
2,704,840 + -1.1
2,704,877 + -0.7
2,704,878 - +0.3
2,704,882 - +1.4
2,704,910 + +0.3
2,704,910 + +1.1
2,704,910 + +0.1
2,704,911 - -0.7
2,704,911 - -1.7
2,705,027 + +1.3
2,705,028 - Pf1N1B4_2387 0.13 +0.5
2,705,245 + Pf1N1B4_2387 0.38 -2.0
2,705,246 - Pf1N1B4_2387 0.38 -1.1
2,705,267 + Pf1N1B4_2387 0.40 -1.3
2,705,268 - Pf1N1B4_2387 0.40 +1.8
2,705,349 + Pf1N1B4_2387 0.49 -0.3
2,705,349 + Pf1N1B4_2387 0.49 +0.6
2,705,349 + Pf1N1B4_2387 0.49 +2.3
2,705,349 + Pf1N1B4_2387 0.49 -0.3
2,705,349 + Pf1N1B4_2387 0.49 +0.3
2,705,349 + Pf1N1B4_2387 0.49 +0.7
2,705,349 + Pf1N1B4_2387 0.49 +1.9
2,705,350 - Pf1N1B4_2387 0.49 -0.7
2,705,350 - Pf1N1B4_2387 0.49 -0.4
2,705,350 - Pf1N1B4_2387 0.49 +0.9
2,705,350 - Pf1N1B4_2387 0.49 -0.0
2,705,544 + Pf1N1B4_2387 0.71 -0.7
2,705,544 + Pf1N1B4_2387 0.71 -1.3
2,705,544 + Pf1N1B4_2387 0.71 +0.9
2,705,545 - Pf1N1B4_2387 0.71 -0.8
2,705,545 - Pf1N1B4_2387 0.71 -0.1
2,705,545 - Pf1N1B4_2387 0.71 +0.3
2,705,601 + Pf1N1B4_2387 0.78 -0.7
2,705,601 + Pf1N1B4_2387 0.78 -0.7
2,705,601 + Pf1N1B4_2387 0.78 -0.9
2,705,602 - Pf1N1B4_2387 0.78 -0.9
2,705,602 - Pf1N1B4_2387 0.78 -1.3
2,705,647 + Pf1N1B4_2387 0.83 +0.7
2,705,647 + Pf1N1B4_2387 0.83 +0.9
2,705,648 - Pf1N1B4_2387 0.83 +0.3
2,705,693 + Pf1N1B4_2387 0.88 +1.9
2,705,693 + Pf1N1B4_2387 0.88 +0.3
2,705,693 + Pf1N1B4_2387 0.88 +1.0
2,705,694 - Pf1N1B4_2387 0.88 +0.7

Or see this region's nucleotide sequence