Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2338 and Pf1N1B4_2339 are separated by 183 nucleotides Pf1N1B4_2339 and Pf1N1B4_2340 overlap by 1 nucleotides Pf1N1B4_2340 and Pf1N1B4_2341 are separated by 21 nucleotides
Pf1N1B4_2338 - Homoserine O-acetyltransferase (EC 2.3.1.31), at 2,654,730 to 2,655,869
_2338
Pf1N1B4_2339 - COG1872, at 2,656,053 to 2,656,349
_2339
Pf1N1B4_2340 - Integral membrane protein YggT, involved in response to extracytoplasmic stress (osmotic shock), at 2,656,349 to 2,656,939
_2340
Pf1N1B4_2341 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 2,656,961 to 2,657,779
_2341
Position (kb)
2656
2657 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2655.062 kb on - strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.070 kb on + strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.071 kb on - strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.106 kb on + strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.107 kb on - strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.169 kb on + strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.170 kb on - strand, within Pf1N1B4_2338 at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.569 kb on + strand at 2655.569 kb on + strand, within Pf1N1B4_2338 at 2655.570 kb on - strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.745 kb on + strand, within Pf1N1B4_2338 at 2655.746 kb on - strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.754 kb on + strand, within Pf1N1B4_2338 at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.766 kb on + strand at 2655.767 kb on - strand at 2655.767 kb on - strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.897 kb on + strand at 2655.898 kb on - strand at 2655.898 kb on - strand at 2655.903 kb on + strand at 2655.903 kb on + strand at 2656.201 kb on + strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.202 kb on - strand, within Pf1N1B4_2339 at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.342 kb on + strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.343 kb on - strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.386 kb on + strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.387 kb on - strand at 2656.389 kb on + strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand at 2656.952 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,655,062 - Pf1N1B4_2338 0.29 +0.5 2,655,070 + Pf1N1B4_2338 0.30 +0.8 2,655,070 + Pf1N1B4_2338 0.30 -2.0 2,655,070 + Pf1N1B4_2338 0.30 -1.7 2,655,070 + Pf1N1B4_2338 0.30 -1.3 2,655,071 - Pf1N1B4_2338 0.30 -0.7 2,655,071 - Pf1N1B4_2338 0.30 -1.3 2,655,106 + Pf1N1B4_2338 0.33 +1.3 2,655,106 + Pf1N1B4_2338 0.33 -0.2 2,655,107 - Pf1N1B4_2338 0.33 +0.3 2,655,107 - Pf1N1B4_2338 0.33 -0.9 2,655,169 + Pf1N1B4_2338 0.39 -1.7 2,655,169 + Pf1N1B4_2338 0.39 +1.6 2,655,169 + Pf1N1B4_2338 0.39 +1.7 2,655,169 + Pf1N1B4_2338 0.39 -1.7 2,655,169 + Pf1N1B4_2338 0.39 -0.8 2,655,169 + Pf1N1B4_2338 0.39 +0.4 2,655,169 + Pf1N1B4_2338 0.39 -0.1 2,655,169 + Pf1N1B4_2338 0.39 -2.0 2,655,169 + Pf1N1B4_2338 0.39 -0.3 2,655,170 - Pf1N1B4_2338 0.39 -1.7 2,655,170 - Pf1N1B4_2338 0.39 -0.5 2,655,170 - Pf1N1B4_2338 0.39 +0.3 2,655,170 - Pf1N1B4_2338 0.39 +1.3 2,655,569 + Pf1N1B4_2338 0.74 -1.3 2,655,569 + -0.7 2,655,569 + Pf1N1B4_2338 0.74 +1.9 2,655,570 - Pf1N1B4_2338 0.74 +0.1 2,655,745 + Pf1N1B4_2338 0.89 +1.5 2,655,745 + Pf1N1B4_2338 0.89 -1.5 2,655,746 - Pf1N1B4_2338 0.89 -0.0 2,655,754 + Pf1N1B4_2338 0.90 +0.3 2,655,754 + Pf1N1B4_2338 0.90 -1.7 2,655,754 + Pf1N1B4_2338 0.90 +1.1 2,655,766 + +0.9 2,655,766 + -0.2 2,655,766 + -0.9 2,655,766 + +0.3 2,655,766 + -1.3 2,655,767 - -1.7 2,655,767 - +0.1 2,655,897 + +0.3 2,655,897 + -0.5 2,655,897 + +2.1 2,655,897 + -0.1 2,655,898 - -0.6 2,655,898 - +0.7 2,655,903 + -0.1 2,655,903 + +0.6 2,656,201 + Pf1N1B4_2339 0.50 -1.0 2,656,202 - Pf1N1B4_2339 0.50 -0.9 2,656,202 - Pf1N1B4_2339 0.50 -1.0 2,656,202 - Pf1N1B4_2339 0.50 -2.7 2,656,342 + +0.5 2,656,342 + -0.9 2,656,342 + +0.6 2,656,343 - -1.4 2,656,343 - -0.8 2,656,343 - -0.5 2,656,343 - +0.1 2,656,386 + -0.3 2,656,386 + +1.3 2,656,386 + -0.6 2,656,387 - -1.7 2,656,387 - +1.7 2,656,387 - +1.6 2,656,387 - -1.7 2,656,387 - +0.3 2,656,387 - -0.7 2,656,389 + +0.3 2,656,952 - -1.0 2,656,952 - -1.7 2,656,952 - -2.0 2,656,952 - -0.7 2,656,952 - -1.1
Or see this region's nucleotide sequence