Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2328 and Pf1N1B4_2329 are separated by 74 nucleotides Pf1N1B4_2329 and Pf1N1B4_2330 are separated by 72 nucleotides Pf1N1B4_2330 and Pf1N1B4_2331 are separated by 60 nucleotides Pf1N1B4_2331 and Pf1N1B4_2332 are separated by 9 nucleotides Pf1N1B4_2332 and Pf1N1B4_2333 are separated by 116 nucleotides
Pf1N1B4_2328 - Monofunctional biosynthetic peptidoglycan transglycosylase (EC 2.4.2.-), at 2,648,347 to 2,649,069
_2328
Pf1N1B4_2329 - COG2363, at 2,649,144 to 2,649,515
_2329
Pf1N1B4_2330 - Sulfur carrier protein ThiS, at 2,649,588 to 2,649,806
_2330
Pf1N1B4_2331 - Thiazole biosynthesis protein ThiG, at 2,649,867 to 2,650,661
_2331
Pf1N1B4_2332 - tRNA (guanine46-N7-)-methyltransferase (EC 2.1.1.33), at 2,650,671 to 2,651,396
_2332
Pf1N1B4_2333 - Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific, at 2,651,513 to 2,651,836
_2333
Position (kb)
2649
2650
2651 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2649.007 kb on + strand at 2649.007 kb on + strand at 2649.058 kb on + strand at 2649.069 kb on + strand at 2649.069 kb on + strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.070 kb on - strand at 2649.105 kb on + strand at 2649.105 kb on + strand at 2649.106 kb on - strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.107 kb on + strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.108 kb on - strand at 2649.276 kb on + strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.277 kb on - strand, within Pf1N1B4_2329 at 2649.290 kb on + strand, within Pf1N1B4_2329 at 2649.310 kb on + strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.311 kb on - strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.402 kb on + strand, within Pf1N1B4_2329 at 2649.784 kb on + strand, within Pf1N1B4_2330 at 2649.852 kb on + strand at 2649.881 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.911 kb on + strand at 2649.912 kb on - strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.915 kb on + strand at 2649.916 kb on - strand at 2649.934 kb on + strand at 2649.934 kb on + strand at 2649.935 kb on - strand at 2649.939 kb on - strand at 2650.083 kb on + strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.084 kb on - strand, within Pf1N1B4_2331 at 2650.098 kb on - strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.284 kb on + strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.285 kb on - strand, within Pf1N1B4_2331 at 2650.591 kb on - strand at 2650.620 kb on + strand at 2650.620 kb on + strand at 2650.620 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.659 kb on + strand at 2650.660 kb on - strand at 2650.660 kb on - strand at 2650.813 kb on + strand, within Pf1N1B4_2332 at 2651.029 kb on - strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.235 kb on + strand, within Pf1N1B4_2332 at 2651.394 kb on + strand at 2651.394 kb on + strand at 2651.504 kb on + strand at 2651.504 kb on + strand at 2651.505 kb on - strand at 2651.505 kb on - strand at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.577 kb on + strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.578 kb on - strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.622 kb on + strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333 at 2651.623 kb on - strand, within Pf1N1B4_2333
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,649,007 + -0.1 2,649,007 + +0.6 2,649,058 + -0.9 2,649,069 + -0.2 2,649,069 + +0.0 2,649,070 - -1.7 2,649,070 - -0.3 2,649,070 - +0.3 2,649,105 + -0.4 2,649,105 + -0.7 2,649,106 - -2.3 2,649,107 + -0.3 2,649,107 + -1.7 2,649,107 + -1.4 2,649,107 + +1.1 2,649,107 + +0.9 2,649,107 + -0.7 2,649,107 + -0.4 2,649,107 + -0.8 2,649,107 + +0.9 2,649,107 + +1.3 2,649,107 + -0.4 2,649,107 + +0.0 2,649,108 - -1.7 2,649,108 - -1.3 2,649,108 - +0.3 2,649,108 - +0.3 2,649,108 - +0.1 2,649,108 - +0.9 2,649,108 - -0.4 2,649,108 - +0.3 2,649,108 - +1.9 2,649,108 - -0.4 2,649,108 - -0.7 2,649,108 - +0.7 2,649,108 - -0.3 2,649,108 - -1.3 2,649,108 - -1.3 2,649,108 - -0.6 2,649,276 + Pf1N1B4_2329 0.35 +1.1 2,649,277 - Pf1N1B4_2329 0.36 -2.3 2,649,277 - Pf1N1B4_2329 0.36 +1.6 2,649,277 - Pf1N1B4_2329 0.36 +0.3 2,649,277 - Pf1N1B4_2329 0.36 -0.1 2,649,277 - Pf1N1B4_2329 0.36 -2.5 2,649,277 - Pf1N1B4_2329 0.36 +0.7 2,649,277 - Pf1N1B4_2329 0.36 -0.7 2,649,290 + Pf1N1B4_2329 0.39 -1.4 2,649,310 + Pf1N1B4_2329 0.45 -1.3 2,649,311 - Pf1N1B4_2329 0.45 -1.8 2,649,311 - Pf1N1B4_2329 0.45 -0.0 2,649,402 + Pf1N1B4_2329 0.69 +0.7 2,649,402 + Pf1N1B4_2329 0.69 -0.7 2,649,402 + Pf1N1B4_2329 0.69 +0.3 2,649,784 + Pf1N1B4_2330 0.89 -1.3 2,649,852 + +0.9 2,649,881 + +0.3 2,649,911 + -1.3 2,649,911 + -0.7 2,649,911 + -0.2 2,649,912 - +1.3 2,649,915 + -0.5 2,649,915 + -0.1 2,649,915 + +2.7 2,649,915 + -0.7 2,649,916 - -1.7 2,649,934 + +1.3 2,649,934 + -1.3 2,649,935 - -0.7 2,649,939 - +0.3 2,650,083 + Pf1N1B4_2331 0.27 -0.7 2,650,084 - Pf1N1B4_2331 0.27 -0.8 2,650,084 - Pf1N1B4_2331 0.27 -0.3 2,650,098 - Pf1N1B4_2331 0.29 -0.7 2,650,284 + Pf1N1B4_2331 0.52 -1.2 2,650,284 + Pf1N1B4_2331 0.52 -0.3 2,650,284 + Pf1N1B4_2331 0.52 -0.3 2,650,284 + Pf1N1B4_2331 0.52 -1.7 2,650,284 + Pf1N1B4_2331 0.52 -0.7 2,650,285 - Pf1N1B4_2331 0.53 -1.3 2,650,285 - Pf1N1B4_2331 0.53 +0.3 2,650,591 - -1.7 2,650,620 + -0.7 2,650,620 + -1.3 2,650,620 + -0.4 2,650,659 + -1.0 2,650,659 + +1.1 2,650,659 + -1.3 2,650,659 + -1.3 2,650,659 + -1.2 2,650,659 + -0.1 2,650,660 - -1.7 2,650,660 - +1.0 2,650,813 + Pf1N1B4_2332 0.20 +0.7 2,651,029 - Pf1N1B4_2332 0.49 -1.7 2,651,235 + Pf1N1B4_2332 0.78 -0.1 2,651,235 + Pf1N1B4_2332 0.78 -1.3 2,651,235 + Pf1N1B4_2332 0.78 -1.0 2,651,394 + -0.3 2,651,394 + -1.3 2,651,504 + +0.1 2,651,504 + +0.9 2,651,505 - +0.3 2,651,505 - +0.0 2,651,577 + Pf1N1B4_2333 0.20 -0.3 2,651,577 + Pf1N1B4_2333 0.20 -0.7 2,651,577 + Pf1N1B4_2333 0.20 +0.9 2,651,577 + Pf1N1B4_2333 0.20 +0.2 2,651,577 + Pf1N1B4_2333 0.20 -0.7 2,651,577 + Pf1N1B4_2333 0.20 +0.7 2,651,577 + Pf1N1B4_2333 0.20 +2.3 2,651,578 - Pf1N1B4_2333 0.20 -2.8 2,651,578 - Pf1N1B4_2333 0.20 +0.3 2,651,578 - Pf1N1B4_2333 0.20 -1.7 2,651,578 - Pf1N1B4_2333 0.20 -0.5 2,651,578 - Pf1N1B4_2333 0.20 -0.8 2,651,578 - Pf1N1B4_2333 0.20 -1.4 2,651,578 - -0.7 2,651,578 - Pf1N1B4_2333 0.20 +0.2 2,651,578 - Pf1N1B4_2333 0.20 -0.1 2,651,578 - Pf1N1B4_2333 0.20 -0.9 2,651,578 - Pf1N1B4_2333 0.20 -1.0 2,651,578 - Pf1N1B4_2333 0.20 +0.3 2,651,578 - Pf1N1B4_2333 0.20 -0.4 2,651,622 + Pf1N1B4_2333 0.34 -0.7 2,651,622 + Pf1N1B4_2333 0.34 -2.5 2,651,622 + Pf1N1B4_2333 0.34 +0.3 2,651,623 - Pf1N1B4_2333 0.34 -0.3 2,651,623 - Pf1N1B4_2333 0.34 -1.7 2,651,623 - Pf1N1B4_2333 0.34 +0.7 2,651,623 - Pf1N1B4_2333 0.34 +0.3 2,651,623 - Pf1N1B4_2333 0.34 -1.7 2,651,623 - Pf1N1B4_2333 0.34 -0.5 2,651,623 - Pf1N1B4_2333 0.34 -0.8 2,651,623 - Pf1N1B4_2333 0.34 -0.7 2,651,623 - Pf1N1B4_2333 0.34 -1.3 2,651,623 - Pf1N1B4_2333 0.34 +0.3 2,651,623 - Pf1N1B4_2333 0.34 -0.5
Or see this region's nucleotide sequence