Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_230 and Pf1N1B4_231 are separated by 469 nucleotides Pf1N1B4_231 and Pf1N1B4_232 are separated by 47 nucleotides Pf1N1B4_232 and Pf1N1B4_233 overlap by 17 nucleotides
Pf1N1B4_230 - FIG00953472: hypothetical protein, at 291,131 to 291,601
_230
Pf1N1B4_231 - Sensor histidine kinase, at 292,071 to 292,964
_231
Pf1N1B4_232 - DNA-binding response regulator, LuxR family, at 293,012 to 293,620
_232
Pf1N1B4_233 - Transcription regulator [contains diacylglycerol kinase catalytic domain], at 293,604 to 294,536
_233
Position (kb) 292
293 Strain fitness (log2 ratio) -2
-1
0
1
2 at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.071 kb on + strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.072 kb on - strand at 291.126 kb on + strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.127 kb on - strand at 291.344 kb on - strand, within Pf1N1B4_230 at 291.505 kb on - strand, within Pf1N1B4_230 at 291.505 kb on - strand, within Pf1N1B4_230 at 291.600 kb on - strand at 291.600 kb on - strand at 291.600 kb on - strand at 291.633 kb on + strand at 291.653 kb on - strand at 291.654 kb on + strand at 291.655 kb on - strand at 291.713 kb on - strand at 291.713 kb on - strand at 291.716 kb on + strand at 291.717 kb on - strand at 291.717 kb on - strand at 291.717 kb on - strand at 291.738 kb on - strand at 291.831 kb on + strand at 291.844 kb on + strand at 291.844 kb on + strand at 291.844 kb on + strand at 291.844 kb on + strand at 291.844 kb on + strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.845 kb on - strand at 291.850 kb on + strand at 291.850 kb on + strand at 291.850 kb on + strand at 291.850 kb on + strand at 291.851 kb on - strand at 291.851 kb on - strand at 291.851 kb on - strand at 291.851 kb on - strand at 291.851 kb on - strand at 291.851 kb on - strand at 291.852 kb on + strand at 291.878 kb on + strand at 291.879 kb on - strand at 291.879 kb on - strand at 291.885 kb on + strand at 291.889 kb on - strand at 292.070 kb on + strand at 292.071 kb on - strand at 292.074 kb on + strand at 292.075 kb on - strand at 292.075 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.095 kb on - strand at 292.148 kb on + strand at 292.148 kb on + strand at 292.149 kb on - strand at 292.193 kb on - strand, within Pf1N1B4_231 at 292.224 kb on - strand, within Pf1N1B4_231 at 292.561 kb on + strand, within Pf1N1B4_231 at 292.561 kb on + strand at 292.562 kb on - strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.580 kb on + strand, within Pf1N1B4_231 at 292.581 kb on - strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.682 kb on + strand, within Pf1N1B4_231 at 292.683 kb on - strand at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.684 kb on + strand, within Pf1N1B4_231 at 292.706 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.849 kb on + strand, within Pf1N1B4_231 at 292.931 kb on - strand at 292.949 kb on + strand at 292.950 kb on - strand at 292.969 kb on + strand at 292.969 kb on + strand at 292.970 kb on - strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.974 kb on + strand at 292.975 kb on - strand at 292.975 kb on - strand at 292.999 kb on + strand at 293.049 kb on + strand at 293.125 kb on + strand at 293.558 kb on + strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.559 kb on - strand, within Pf1N1B4_232 at 293.735 kb on + strand, within Pf1N1B4_233 at 293.736 kb on - strand, within Pf1N1B4_233 at 293.736 kb on - strand at 293.737 kb on + strand at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand, within Pf1N1B4_233 at 293.738 kb on - strand at 293.738 kb on - strand, within Pf1N1B4_233
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 291,071 + +1.1 291,071 + -2.2 291,071 + +0.4 291,071 + +1.4 291,071 + -2.6 291,072 - -1.2 291,072 - -1.2 291,072 - +1.8 291,072 - +0.6 291,072 - +0.8 291,072 - -1.6 291,072 - -0.2 291,072 - +0.8 291,072 - +0.5 291,072 - +0.2 291,126 + -0.2 291,127 - -0.9 291,127 - -1.4 291,127 - +0.4 291,127 - -0.2 291,127 - -1.6 291,127 - +0.3 291,127 - -0.2 291,127 - +0.9 291,127 - -2.0 291,127 - +1.0 291,344 - Pf1N1B4_230 0.45 +0.4 291,505 - Pf1N1B4_230 0.79 +0.1 291,505 - Pf1N1B4_230 0.79 +0.2 291,600 - +0.1 291,600 - -0.8 291,600 - -0.7 291,633 + -1.2 291,653 - +0.8 291,654 + -1.2 291,655 - -1.6 291,713 - +1.4 291,713 - -1.5 291,716 + +0.4 291,717 - -1.0 291,717 - +0.5 291,717 - +0.1 291,738 - +1.8 291,831 + +0.4 291,844 + +1.6 291,844 + -1.2 291,844 + -0.8 291,844 + -1.2 291,844 + -0.2 291,845 - -1.0 291,845 - -0.5 291,845 - +0.4 291,845 - +0.5 291,845 - -1.2 291,845 - -0.8 291,845 - -2.0 291,850 + -0.2 291,850 + -0.2 291,850 + +1.8 291,850 + +0.4 291,851 - -0.2 291,851 - -0.8 291,851 - -0.8 291,851 - +0.2 291,851 - -1.2 291,851 - -2.6 291,852 + -0.2 291,878 + -0.6 291,879 - -1.2 291,879 - -0.2 291,885 + -0.2 291,889 - -1.0 292,070 + -0.2 292,071 - +1.2 292,074 + -0.0 292,075 - +2.1 292,075 - -0.6 292,095 - -0.6 292,095 - -1.8 292,095 - -0.2 292,148 + -0.2 292,148 + +2.4 292,149 - +0.5 292,193 - Pf1N1B4_231 0.14 -2.4 292,224 - Pf1N1B4_231 0.17 -0.6 292,561 + Pf1N1B4_231 0.55 -1.0 292,561 + -0.2 292,562 - Pf1N1B4_231 0.55 +2.4 292,580 + Pf1N1B4_231 0.57 -2.2 292,580 + Pf1N1B4_231 0.57 -0.6 292,580 + Pf1N1B4_231 0.57 -1.2 292,581 - Pf1N1B4_231 0.57 -1.7 292,682 + Pf1N1B4_231 0.68 -1.2 292,682 + Pf1N1B4_231 0.68 +0.4 292,683 - +0.8 292,684 + Pf1N1B4_231 0.69 -1.2 292,684 + Pf1N1B4_231 0.69 -0.6 292,684 + Pf1N1B4_231 0.69 -1.8 292,706 + Pf1N1B4_231 0.71 +0.8 292,849 + Pf1N1B4_231 0.87 -0.7 292,849 + Pf1N1B4_231 0.87 -2.6 292,931 - -0.8 292,949 + -1.2 292,950 - +0.4 292,969 + -1.8 292,969 + -0.2 292,970 - -0.3 292,974 + -1.2 292,974 + -0.6 292,974 + -1.2 292,974 + -0.2 292,975 - +2.4 292,975 - -0.6 292,999 + -1.5 293,049 + -0.2 293,125 + -0.2 293,558 + Pf1N1B4_232 0.90 -0.5 293,559 - Pf1N1B4_232 0.90 +0.8 293,559 - Pf1N1B4_232 0.90 -0.9 293,735 + Pf1N1B4_233 0.14 +0.8 293,736 - Pf1N1B4_233 0.14 -1.8 293,736 - -0.2 293,737 + +0.4 293,738 - -0.2 293,738 - Pf1N1B4_233 0.14 +1.4 293,738 - Pf1N1B4_233 0.14 -1.8 293,738 - Pf1N1B4_233 0.14 +1.0 293,738 - Pf1N1B4_233 0.14 -0.0 293,738 - -1.8 293,738 - Pf1N1B4_233 0.14 -2.6
Or see this region's nucleotide sequence