Experiment: D-Cycloserine 0.1 mg/ml 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2209 and Pf1N1B4_2210 are separated by 208 nucleotides Pf1N1B4_2210 and Pf1N1B4_2211 overlap by 14 nucleotides Pf1N1B4_2211 and Pf1N1B4_2212 overlap by 4 nucleotides 
        Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381 
        _2209 
         
        
        Pf1N1B4_2210 - Glutamate transport ATP-binding protein, at 2,524,590 to 2,525,327 
        _2210 
         
        
        Pf1N1B4_2211 - Putative amino acid ABC transporter, permease protein, at 2,525,314 to 2,525,964 
        _2211 
         
        
        Pf1N1B4_2212 - amino acid ABC transporter, permease protein, at 2,525,961 to 2,526,632 
        _2212 
         Position (kb) 2524 
2525 
2526 Strain fitness (log2 ratio) -4 
-3 
-2 
-1 
0 
1 
2 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.811 kb on - strand, within Pf1N1B4_2209 at 2523.855 kb on + strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.868 kb on - strand, within Pf1N1B4_2209 at 2524.008 kb on + strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.151 kb on - strand, within Pf1N1B4_2209 at 2524.512 kb on + strand at 2524.548 kb on + strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2525.131 kb on + strand, within Pf1N1B4_2210 at 2525.132 kb on - strand, within Pf1N1B4_2210 at 2525.132 kb on - strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.369 kb on + strand at 2525.385 kb on - strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.567 kb on + strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.666 kb on + strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.880 kb on + strand, within Pf1N1B4_2211 at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.986 kb on + strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2526.040 kb on + strand at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction D-Cycloserine 0.1 mg/ml remove 2,523,810 +  Pf1N1B4_2209 0.62  -4.1 2,523,810 +  Pf1N1B4_2209 0.62  -3.3 2,523,810 +  Pf1N1B4_2209 0.62  -3.2 2,523,811 -  Pf1N1B4_2209 0.62  -1.6 2,523,855 +  Pf1N1B4_2209 0.65  -2.8 2,523,856 -  Pf1N1B4_2209 0.65  -2.9 2,523,856 -  Pf1N1B4_2209 0.65  -2.3 2,523,867 +  Pf1N1B4_2209 0.66  -3.5 2,523,867 +  Pf1N1B4_2209 0.66  -2.6 2,523,867 +  Pf1N1B4_2209 0.66  -2.2 2,523,867 +  Pf1N1B4_2209 0.66  -2.3 2,523,867 +  Pf1N1B4_2209 0.66  -1.9 2,523,867 +  Pf1N1B4_2209 0.66  -2.9 2,523,867 +  Pf1N1B4_2209 0.66  -2.2 2,523,868 -  Pf1N1B4_2209 0.66  -3.8 2,524,008 +  Pf1N1B4_2209 0.75  -1.6 2,524,009 -  Pf1N1B4_2209 0.75  -2.8 2,524,009 -  Pf1N1B4_2209 0.75  -2.3 2,524,151 -  Pf1N1B4_2209 0.85  -2.8 2,524,512 +  +1.4 2,524,548 +  -0.7 2,524,548 +  -0.1 2,524,549 -  -0.8 2,524,656 +  +0.9 2,524,656 +  +1.0 2,524,656 +  +0.2 2,524,657 -  -1.6 2,524,657 -  -1.9 2,524,657 -  +1.2 2,524,657 -  +0.4 2,525,131 +  Pf1N1B4_2210 0.73  -2.2 2,525,132 -  Pf1N1B4_2210 0.73  -0.7 2,525,132 -  -1.2 2,525,293 +  -0.3 2,525,293 +  +0.4 2,525,293 +  -0.0 2,525,293 +  -1.6 2,525,293 +  -0.0 2,525,293 +  -1.8 2,525,294 -  -1.6 2,525,294 -  +0.4 2,525,294 -  +0.0 2,525,294 -  +0.1 2,525,294 -  -0.8 2,525,294 -  -1.9 2,525,294 -  -1.2 2,525,297 +  -1.1 2,525,297 +  +1.4 2,525,297 +  -1.6 2,525,298 -  -0.0 2,525,298 -  -1.2 2,525,298 -  -0.2 2,525,298 -  +0.4 2,525,298 -  +2.7 2,525,298 -  +0.8 2,525,298 -  +0.4 2,525,298 -  -0.4 2,525,298 -  -0.6 2,525,298 -  -0.4 2,525,315 +  +1.1 2,525,315 +  +1.4 2,525,315 +  +0.4 2,525,316 -  -1.6 2,525,316 -  -0.5 2,525,316 -  -0.6 2,525,316 -  -2.2 2,525,369 +  +1.1 2,525,385 -  Pf1N1B4_2211 0.11  -1.2 2,525,405 +  Pf1N1B4_2211 0.14  -1.2 2,525,405 +  Pf1N1B4_2211 0.14  +0.8 2,525,567 +  Pf1N1B4_2211 0.39  -0.0 2,525,568 -  Pf1N1B4_2211 0.39  -0.2 2,525,568 -  Pf1N1B4_2211 0.39  +0.6 2,525,666 +  Pf1N1B4_2211 0.54  -0.2 2,525,667 -  Pf1N1B4_2211 0.54  +0.8 2,525,667 -  Pf1N1B4_2211 0.54  -0.6 2,525,798 +  Pf1N1B4_2211 0.74  -0.0 2,525,798 +  Pf1N1B4_2211 0.74  +0.4 2,525,798 +  Pf1N1B4_2211 0.74  +1.0 2,525,798 +  Pf1N1B4_2211 0.74  +0.8 2,525,798 +  Pf1N1B4_2211 0.74  +1.2 2,525,799 -  Pf1N1B4_2211 0.75  -1.2 2,525,799 -  Pf1N1B4_2211 0.75  +0.5 2,525,799 -  Pf1N1B4_2211 0.75  +0.4 2,525,799 -  Pf1N1B4_2211 0.75  +1.4 2,525,880 +  Pf1N1B4_2211 0.87  -0.6 2,525,982 +  +0.1 2,525,982 +  +0.8 2,525,982 +  +0.4 2,525,982 +  -1.2 2,525,982 +  -1.2 2,525,982 +  +2.0 2,525,982 +  +0.4 2,525,982 +  -0.6 2,525,982 +  -0.6 2,525,982 +  +0.4 2,525,982 +  -1.8 2,525,982 +  +0.4 2,525,982 +  -0.8 2,525,982 +  -1.2 2,525,982 +  +1.0 2,525,982 +  -0.1 2,525,982 +  +1.1 2,525,982 +  -0.4 2,525,983 -  +0.2 2,525,983 -  -1.3 2,525,983 -  -0.0 2,525,983 -  -0.1 2,525,983 -  +0.1 2,525,983 -  -1.4 2,525,983 -  +0.2 2,525,983 -  +0.4 2,525,983 -  -0.3 2,525,983 -  +0.8 2,525,986 +  -0.6 2,525,987 -  -0.6 2,525,987 -  -0.6 2,525,987 -  -1.6 2,525,987 -  -0.7 2,525,987 -  -0.2 2,525,987 -  +0.4 2,526,040 +  +0.4 2,526,040 +  Pf1N1B4_2212 0.12  -0.0 2,526,040 +  Pf1N1B4_2212 0.12  -1.0 2,526,040 +  Pf1N1B4_2212 0.12  -1.2 2,526,040 +  Pf1N1B4_2212 0.12  +0.7 2,526,041 -  Pf1N1B4_2212 0.12  -0.9 2,526,041 -  Pf1N1B4_2212 0.12  +0.7 2,526,041 -  Pf1N1B4_2212 0.12  +0.2 2,526,041 -  Pf1N1B4_2212 0.12  -1.3 2,526,041 -  Pf1N1B4_2212 0.12  +0.4 2,526,041 -  Pf1N1B4_2212 0.12  -1.2 2,526,061 +  Pf1N1B4_2212 0.15  +0.1 2,526,061 +  Pf1N1B4_2212 0.15  +0.2 2,526,061 +  Pf1N1B4_2212 0.15  +0.8 2,526,061 +  Pf1N1B4_2212 0.15  -0.0 2,526,061 +  Pf1N1B4_2212 0.15  -1.2 2,526,061 +  Pf1N1B4_2212 0.15  +0.1 2,526,062 -  Pf1N1B4_2212 0.15  -1.2 2,526,062 -  Pf1N1B4_2212 0.15  +1.4 2,526,062 -  Pf1N1B4_2212 0.15  -0.4 2,526,084 -  Pf1N1B4_2212 0.18  +0.4 2,526,084 -  Pf1N1B4_2212 0.18  -1.2 2,526,323 -  Pf1N1B4_2212 0.54  -0.2 2,526,323 -  Pf1N1B4_2212 0.54  +0.4 
Or see this region's nucleotide sequence