Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2209 and Pf1N1B4_2210 are separated by 208 nucleotides Pf1N1B4_2210 and Pf1N1B4_2211 overlap by 14 nucleotides Pf1N1B4_2211 and Pf1N1B4_2212 overlap by 4 nucleotides
Pf1N1B4_2209 - Exopolyphosphatase (EC 3.6.1.11), at 2,522,879 to 2,524,381
_2209
Pf1N1B4_2210 - Glutamate transport ATP-binding protein, at 2,524,590 to 2,525,327
_2210
Pf1N1B4_2211 - Putative amino acid ABC transporter, permease protein, at 2,525,314 to 2,525,964
_2211
Pf1N1B4_2212 - amino acid ABC transporter, permease protein, at 2,525,961 to 2,526,632
_2212
Position (kb)
2524
2525
2526 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.810 kb on + strand, within Pf1N1B4_2209 at 2523.811 kb on - strand, within Pf1N1B4_2209 at 2523.855 kb on + strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.856 kb on - strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.867 kb on + strand, within Pf1N1B4_2209 at 2523.868 kb on - strand, within Pf1N1B4_2209 at 2524.008 kb on + strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.009 kb on - strand, within Pf1N1B4_2209 at 2524.151 kb on - strand, within Pf1N1B4_2209 at 2524.512 kb on + strand at 2524.548 kb on + strand at 2524.548 kb on + strand at 2524.549 kb on - strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.656 kb on + strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2524.657 kb on - strand at 2525.131 kb on + strand, within Pf1N1B4_2210 at 2525.132 kb on - strand, within Pf1N1B4_2210 at 2525.132 kb on - strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.293 kb on + strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.294 kb on - strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.297 kb on + strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.298 kb on - strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.315 kb on + strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.316 kb on - strand at 2525.369 kb on + strand at 2525.385 kb on - strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.405 kb on + strand, within Pf1N1B4_2211 at 2525.567 kb on + strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.568 kb on - strand, within Pf1N1B4_2211 at 2525.666 kb on + strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.667 kb on - strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.798 kb on + strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.799 kb on - strand, within Pf1N1B4_2211 at 2525.880 kb on + strand, within Pf1N1B4_2211 at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.982 kb on + strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.983 kb on - strand at 2525.986 kb on + strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2525.987 kb on - strand at 2526.040 kb on + strand at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.040 kb on + strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.041 kb on - strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.061 kb on + strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.062 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.084 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212 at 2526.323 kb on - strand, within Pf1N1B4_2212
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,523,810 + Pf1N1B4_2209 0.62 -4.1 2,523,810 + Pf1N1B4_2209 0.62 -3.3 2,523,810 + Pf1N1B4_2209 0.62 -3.2 2,523,811 - Pf1N1B4_2209 0.62 -1.6 2,523,855 + Pf1N1B4_2209 0.65 -2.8 2,523,856 - Pf1N1B4_2209 0.65 -2.9 2,523,856 - Pf1N1B4_2209 0.65 -2.3 2,523,867 + Pf1N1B4_2209 0.66 -3.5 2,523,867 + Pf1N1B4_2209 0.66 -2.6 2,523,867 + Pf1N1B4_2209 0.66 -2.2 2,523,867 + Pf1N1B4_2209 0.66 -2.3 2,523,867 + Pf1N1B4_2209 0.66 -1.9 2,523,867 + Pf1N1B4_2209 0.66 -2.9 2,523,867 + Pf1N1B4_2209 0.66 -2.2 2,523,868 - Pf1N1B4_2209 0.66 -3.8 2,524,008 + Pf1N1B4_2209 0.75 -1.6 2,524,009 - Pf1N1B4_2209 0.75 -2.8 2,524,009 - Pf1N1B4_2209 0.75 -2.3 2,524,151 - Pf1N1B4_2209 0.85 -2.8 2,524,512 + +1.4 2,524,548 + -0.7 2,524,548 + -0.1 2,524,549 - -0.8 2,524,656 + +0.9 2,524,656 + +1.0 2,524,656 + +0.2 2,524,657 - -1.6 2,524,657 - -1.9 2,524,657 - +1.2 2,524,657 - +0.4 2,525,131 + Pf1N1B4_2210 0.73 -2.2 2,525,132 - Pf1N1B4_2210 0.73 -0.7 2,525,132 - -1.2 2,525,293 + -0.3 2,525,293 + +0.4 2,525,293 + -0.0 2,525,293 + -1.6 2,525,293 + -0.0 2,525,293 + -1.8 2,525,294 - -1.6 2,525,294 - +0.4 2,525,294 - +0.0 2,525,294 - +0.1 2,525,294 - -0.8 2,525,294 - -1.9 2,525,294 - -1.2 2,525,297 + -1.1 2,525,297 + +1.4 2,525,297 + -1.6 2,525,298 - -0.0 2,525,298 - -1.2 2,525,298 - -0.2 2,525,298 - +0.4 2,525,298 - +2.7 2,525,298 - +0.8 2,525,298 - +0.4 2,525,298 - -0.4 2,525,298 - -0.6 2,525,298 - -0.4 2,525,315 + +1.1 2,525,315 + +1.4 2,525,315 + +0.4 2,525,316 - -1.6 2,525,316 - -0.5 2,525,316 - -0.6 2,525,316 - -2.2 2,525,369 + +1.1 2,525,385 - Pf1N1B4_2211 0.11 -1.2 2,525,405 + Pf1N1B4_2211 0.14 -1.2 2,525,405 + Pf1N1B4_2211 0.14 +0.8 2,525,567 + Pf1N1B4_2211 0.39 -0.0 2,525,568 - Pf1N1B4_2211 0.39 -0.2 2,525,568 - Pf1N1B4_2211 0.39 +0.6 2,525,666 + Pf1N1B4_2211 0.54 -0.2 2,525,667 - Pf1N1B4_2211 0.54 +0.8 2,525,667 - Pf1N1B4_2211 0.54 -0.6 2,525,798 + Pf1N1B4_2211 0.74 -0.0 2,525,798 + Pf1N1B4_2211 0.74 +0.4 2,525,798 + Pf1N1B4_2211 0.74 +1.0 2,525,798 + Pf1N1B4_2211 0.74 +0.8 2,525,798 + Pf1N1B4_2211 0.74 +1.2 2,525,799 - Pf1N1B4_2211 0.75 -1.2 2,525,799 - Pf1N1B4_2211 0.75 +0.5 2,525,799 - Pf1N1B4_2211 0.75 +0.4 2,525,799 - Pf1N1B4_2211 0.75 +1.4 2,525,880 + Pf1N1B4_2211 0.87 -0.6 2,525,982 + +0.1 2,525,982 + +0.8 2,525,982 + +0.4 2,525,982 + -1.2 2,525,982 + -1.2 2,525,982 + +2.0 2,525,982 + +0.4 2,525,982 + -0.6 2,525,982 + -0.6 2,525,982 + +0.4 2,525,982 + -1.8 2,525,982 + +0.4 2,525,982 + -0.8 2,525,982 + -1.2 2,525,982 + +1.0 2,525,982 + -0.1 2,525,982 + +1.1 2,525,982 + -0.4 2,525,983 - +0.2 2,525,983 - -1.3 2,525,983 - -0.0 2,525,983 - -0.1 2,525,983 - +0.1 2,525,983 - -1.4 2,525,983 - +0.2 2,525,983 - +0.4 2,525,983 - -0.3 2,525,983 - +0.8 2,525,986 + -0.6 2,525,987 - -0.6 2,525,987 - -0.6 2,525,987 - -1.6 2,525,987 - -0.7 2,525,987 - -0.2 2,525,987 - +0.4 2,526,040 + +0.4 2,526,040 + Pf1N1B4_2212 0.12 -0.0 2,526,040 + Pf1N1B4_2212 0.12 -1.0 2,526,040 + Pf1N1B4_2212 0.12 -1.2 2,526,040 + Pf1N1B4_2212 0.12 +0.7 2,526,041 - Pf1N1B4_2212 0.12 -0.9 2,526,041 - Pf1N1B4_2212 0.12 +0.7 2,526,041 - Pf1N1B4_2212 0.12 +0.2 2,526,041 - Pf1N1B4_2212 0.12 -1.3 2,526,041 - Pf1N1B4_2212 0.12 +0.4 2,526,041 - Pf1N1B4_2212 0.12 -1.2 2,526,061 + Pf1N1B4_2212 0.15 +0.1 2,526,061 + Pf1N1B4_2212 0.15 +0.2 2,526,061 + Pf1N1B4_2212 0.15 +0.8 2,526,061 + Pf1N1B4_2212 0.15 -0.0 2,526,061 + Pf1N1B4_2212 0.15 -1.2 2,526,061 + Pf1N1B4_2212 0.15 +0.1 2,526,062 - Pf1N1B4_2212 0.15 -1.2 2,526,062 - Pf1N1B4_2212 0.15 +1.4 2,526,062 - Pf1N1B4_2212 0.15 -0.4 2,526,084 - Pf1N1B4_2212 0.18 +0.4 2,526,084 - Pf1N1B4_2212 0.18 -1.2 2,526,323 - Pf1N1B4_2212 0.54 -0.2 2,526,323 - Pf1N1B4_2212 0.54 +0.4
Or see this region's nucleotide sequence