Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2196

Experiment: D-Cycloserine 0.1 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2194 and Pf1N1B4_2195 overlap by 1 nucleotidesPf1N1B4_2195 and Pf1N1B4_2196 are separated by 255 nucleotidesPf1N1B4_2196 and Pf1N1B4_2197 are separated by 158 nucleotidesPf1N1B4_2197 and Pf1N1B4_2198 are separated by 21 nucleotides Pf1N1B4_2194 - Glutathione-regulated potassium-efflux system ATP-binding protein, at 2,505,571 to 2,507,481 _2194 Pf1N1B4_2195 - Small-conductance mechanosensitive channel, at 2,507,481 to 2,508,062 _2195 Pf1N1B4_2196 - Homoserine/homoserine lactone efflux protein, at 2,508,318 to 2,508,950 _2196 Pf1N1B4_2197 - FIG00957574: hypothetical protein, at 2,509,109 to 2,509,834 _2197 Pf1N1B4_2198 - Lipoprotein YcfM, part of a salvage pathway of unknown substrate, at 2,509,856 to 2,510,443 _2198 Position (kb) 2508 2509Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2507.335 kb on + strandat 2507.335 kb on + strandat 2507.335 kb on + strandat 2507.335 kb on + strandat 2507.336 kb on - strandat 2507.568 kb on + strand, within Pf1N1B4_2195at 2507.568 kb on + strand, within Pf1N1B4_2195at 2507.569 kb on - strandat 2507.592 kb on - strand, within Pf1N1B4_2195at 2507.828 kb on + strand, within Pf1N1B4_2195at 2507.925 kb on + strand, within Pf1N1B4_2195at 2507.926 kb on - strand, within Pf1N1B4_2195at 2508.060 kb on + strandat 2508.060 kb on + strandat 2508.060 kb on + strandat 2508.060 kb on + strandat 2508.214 kb on + strandat 2508.278 kb on + strandat 2508.278 kb on + strandat 2508.278 kb on + strandat 2508.278 kb on + strandat 2508.279 kb on - strandat 2508.283 kb on + strandat 2508.283 kb on + strandat 2508.284 kb on - strandat 2508.284 kb on - strandat 2508.284 kb on - strandat 2508.284 kb on - strandat 2508.284 kb on - strandat 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.534 kb on + strand, within Pf1N1B4_2196at 2508.535 kb on - strand, within Pf1N1B4_2196at 2508.561 kb on + strand, within Pf1N1B4_2196at 2508.562 kb on - strand, within Pf1N1B4_2196at 2508.571 kb on - strand, within Pf1N1B4_2196at 2508.608 kb on + strand, within Pf1N1B4_2196at 2508.608 kb on + strand, within Pf1N1B4_2196at 2508.608 kb on + strand, within Pf1N1B4_2196at 2508.948 kb on + strandat 2508.948 kb on + strandat 2509.106 kb on + strandat 2509.106 kb on + strandat 2509.107 kb on - strandat 2509.250 kb on + strand, within Pf1N1B4_2197at 2509.251 kb on - strand, within Pf1N1B4_2197at 2509.251 kb on - strand, within Pf1N1B4_2197at 2509.251 kb on - strand, within Pf1N1B4_2197at 2509.350 kb on + strand, within Pf1N1B4_2197at 2509.350 kb on + strand, within Pf1N1B4_2197at 2509.351 kb on - strand, within Pf1N1B4_2197at 2509.351 kb on - strand, within Pf1N1B4_2197at 2509.351 kb on - strand, within Pf1N1B4_2197at 2509.351 kb on - strand, within Pf1N1B4_2197at 2509.410 kb on + strand, within Pf1N1B4_2197at 2509.411 kb on - strand, within Pf1N1B4_2197at 2509.411 kb on - strand, within Pf1N1B4_2197at 2509.411 kb on - strand, within Pf1N1B4_2197at 2509.482 kb on + strand, within Pf1N1B4_2197at 2509.482 kb on + strand, within Pf1N1B4_2197at 2509.483 kb on - strand, within Pf1N1B4_2197at 2509.483 kb on - strand, within Pf1N1B4_2197at 2509.530 kb on + strand, within Pf1N1B4_2197at 2509.530 kb on + strand, within Pf1N1B4_2197at 2509.530 kb on + strand, within Pf1N1B4_2197at 2509.530 kb on + strand, within Pf1N1B4_2197at 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strandat 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strand, within Pf1N1B4_2197at 2509.531 kb on - strandat 2509.557 kb on + strand, within Pf1N1B4_2197at 2509.558 kb on - strand, within Pf1N1B4_2197at 2509.558 kb on - strand, within Pf1N1B4_2197at 2509.627 kb on + strand, within Pf1N1B4_2197at 2509.627 kb on + strand, within Pf1N1B4_2197at 2509.628 kb on - strandat 2509.628 kb on - strand, within Pf1N1B4_2197at 2509.650 kb on + strand, within Pf1N1B4_2197at 2509.650 kb on + strandat 2509.651 kb on - strand, within Pf1N1B4_2197at 2509.651 kb on - strand, within Pf1N1B4_2197at 2509.651 kb on - strand, within Pf1N1B4_2197at 2509.677 kb on + strand, within Pf1N1B4_2197at 2509.701 kb on + strand, within Pf1N1B4_2197at 2509.702 kb on - strand, within Pf1N1B4_2197at 2509.704 kb on + strand, within Pf1N1B4_2197at 2509.704 kb on + strand, within Pf1N1B4_2197at 2509.704 kb on + strand, within Pf1N1B4_2197at 2509.705 kb on - strand, within Pf1N1B4_2197at 2509.746 kb on + strandat 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strandat 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strandat 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strandat 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.746 kb on + strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.747 kb on - strand, within Pf1N1B4_2197at 2509.767 kb on + strandat 2509.944 kb on + strand, within Pf1N1B4_2198at 2509.944 kb on + strand, within Pf1N1B4_2198at 2509.944 kb on + strand, within Pf1N1B4_2198at 2509.945 kb on - strand, within Pf1N1B4_2198

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml
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2,507,335 + +1.7
2,507,335 + +1.0
2,507,335 + +0.4
2,507,335 + -0.6
2,507,336 - -0.8
2,507,568 + Pf1N1B4_2195 0.15 +1.1
2,507,568 + Pf1N1B4_2195 0.15 -1.3
2,507,569 - +0.4
2,507,592 - Pf1N1B4_2195 0.19 +0.4
2,507,828 + Pf1N1B4_2195 0.60 +0.4
2,507,925 + Pf1N1B4_2195 0.76 -1.0
2,507,926 - Pf1N1B4_2195 0.76 -0.8
2,508,060 + +0.8
2,508,060 + +1.4
2,508,060 + +0.9
2,508,060 + +0.4
2,508,214 + -0.2
2,508,278 + -0.3
2,508,278 + -0.4
2,508,278 + -0.9
2,508,278 + +1.4
2,508,279 - -0.6
2,508,283 + -0.9
2,508,283 + +1.0
2,508,284 - -0.6
2,508,284 - -0.7
2,508,284 - +0.7
2,508,284 - +0.4
2,508,284 - -0.6
2,508,534 + Pf1N1B4_2196 0.34 -1.8
2,508,534 + Pf1N1B4_2196 0.34 +0.4
2,508,534 + Pf1N1B4_2196 0.34 -0.9
2,508,534 + Pf1N1B4_2196 0.34 +0.4
2,508,534 + Pf1N1B4_2196 0.34 -0.6
2,508,534 + Pf1N1B4_2196 0.34 -0.6
2,508,534 + Pf1N1B4_2196 0.34 -1.6
2,508,535 - Pf1N1B4_2196 0.34 -1.9
2,508,561 + Pf1N1B4_2196 0.38 -0.2
2,508,562 - Pf1N1B4_2196 0.39 -0.2
2,508,571 - Pf1N1B4_2196 0.40 +0.4
2,508,608 + Pf1N1B4_2196 0.46 -1.9
2,508,608 + Pf1N1B4_2196 0.46 +0.1
2,508,608 + Pf1N1B4_2196 0.46 -1.0
2,508,948 + -1.7
2,508,948 + -0.6
2,509,106 + +0.1
2,509,106 + -1.9
2,509,107 - +2.4
2,509,250 + Pf1N1B4_2197 0.19 -0.6
2,509,251 - Pf1N1B4_2197 0.20 +0.4
2,509,251 - Pf1N1B4_2197 0.20 -1.3
2,509,251 - Pf1N1B4_2197 0.20 +1.7
2,509,350 + Pf1N1B4_2197 0.33 +2.4
2,509,350 + Pf1N1B4_2197 0.33 -0.3
2,509,351 - Pf1N1B4_2197 0.33 +1.7
2,509,351 - Pf1N1B4_2197 0.33 +0.4
2,509,351 - Pf1N1B4_2197 0.33 +0.3
2,509,351 - Pf1N1B4_2197 0.33 -1.2
2,509,410 + Pf1N1B4_2197 0.41 +0.1
2,509,411 - Pf1N1B4_2197 0.42 -1.2
2,509,411 - Pf1N1B4_2197 0.42 +0.4
2,509,411 - Pf1N1B4_2197 0.42 +0.1
2,509,482 + Pf1N1B4_2197 0.51 -1.3
2,509,482 + Pf1N1B4_2197 0.51 -1.6
2,509,483 - Pf1N1B4_2197 0.52 -1.6
2,509,483 - Pf1N1B4_2197 0.52 -0.6
2,509,530 + Pf1N1B4_2197 0.58 +0.3
2,509,530 + Pf1N1B4_2197 0.58 -1.9
2,509,530 + Pf1N1B4_2197 0.58 -0.3
2,509,530 + Pf1N1B4_2197 0.58 -1.2
2,509,531 - Pf1N1B4_2197 0.58 +1.4
2,509,531 - Pf1N1B4_2197 0.58 +0.4
2,509,531 - Pf1N1B4_2197 0.58 -0.7
2,509,531 - +0.4
2,509,531 - Pf1N1B4_2197 0.58 -1.4
2,509,531 - Pf1N1B4_2197 0.58 -0.0
2,509,531 - Pf1N1B4_2197 0.58 -0.3
2,509,531 - -0.6
2,509,557 + Pf1N1B4_2197 0.62 +1.4
2,509,558 - Pf1N1B4_2197 0.62 +0.4
2,509,558 - Pf1N1B4_2197 0.62 -1.6
2,509,627 + Pf1N1B4_2197 0.71 -1.8
2,509,627 + Pf1N1B4_2197 0.71 +0.4
2,509,628 - +0.4
2,509,628 - Pf1N1B4_2197 0.71 -0.2
2,509,650 + Pf1N1B4_2197 0.75 +2.0
2,509,650 + -1.2
2,509,651 - Pf1N1B4_2197 0.75 -1.6
2,509,651 - Pf1N1B4_2197 0.75 -0.2
2,509,651 - Pf1N1B4_2197 0.75 -0.9
2,509,677 + Pf1N1B4_2197 0.78 -0.0
2,509,701 + Pf1N1B4_2197 0.82 -1.6
2,509,702 - Pf1N1B4_2197 0.82 +0.8
2,509,704 + Pf1N1B4_2197 0.82 +2.4
2,509,704 + Pf1N1B4_2197 0.82 -0.2
2,509,704 + Pf1N1B4_2197 0.82 -0.6
2,509,705 - Pf1N1B4_2197 0.82 +0.1
2,509,746 + -1.2
2,509,746 + Pf1N1B4_2197 0.88 -0.3
2,509,746 + Pf1N1B4_2197 0.88 +1.4
2,509,746 + Pf1N1B4_2197 0.88 -0.3
2,509,746 + +2.4
2,509,746 + Pf1N1B4_2197 0.88 -0.2
2,509,746 + Pf1N1B4_2197 0.88 -0.2
2,509,746 + Pf1N1B4_2197 0.88 -0.6
2,509,746 + +0.4
2,509,746 + Pf1N1B4_2197 0.88 -0.2
2,509,746 + Pf1N1B4_2197 0.88 -0.6
2,509,746 + Pf1N1B4_2197 0.88 +0.4
2,509,746 + +0.4
2,509,746 + Pf1N1B4_2197 0.88 +0.4
2,509,746 + Pf1N1B4_2197 0.88 +0.4
2,509,746 + Pf1N1B4_2197 0.88 +0.1
2,509,746 + Pf1N1B4_2197 0.88 +0.1
2,509,746 + Pf1N1B4_2197 0.88 -0.6
2,509,746 + Pf1N1B4_2197 0.88 -2.2
2,509,746 + Pf1N1B4_2197 0.88 +0.7
2,509,746 + Pf1N1B4_2197 0.88 -1.6
2,509,747 - Pf1N1B4_2197 0.88 -2.9
2,509,747 - Pf1N1B4_2197 0.88 -0.2
2,509,747 - Pf1N1B4_2197 0.88 -1.2
2,509,747 - Pf1N1B4_2197 0.88 +0.1
2,509,747 - Pf1N1B4_2197 0.88 -0.4
2,509,747 - Pf1N1B4_2197 0.88 +2.0
2,509,747 - Pf1N1B4_2197 0.88 -0.8
2,509,747 - Pf1N1B4_2197 0.88 -0.1
2,509,767 + -0.3
2,509,944 + Pf1N1B4_2198 0.15 -1.2
2,509,944 + Pf1N1B4_2198 0.15 +0.2
2,509,944 + Pf1N1B4_2198 0.15 -1.6
2,509,945 - Pf1N1B4_2198 0.15 +0.7

Or see this region's nucleotide sequence