Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2189 and Pf1N1B4_2190 are separated by 179 nucleotides Pf1N1B4_2190 and Pf1N1B4_2191 are separated by 62 nucleotides Pf1N1B4_2191 and Pf1N1B4_2192 are separated by 192 nucleotides
Pf1N1B4_2189 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 2,501,816 to 2,502,367
_2189
Pf1N1B4_2190 - Regulator of sigma D, at 2,502,547 to 2,503,005
_2190
Pf1N1B4_2191 - Macrophage infectivity potentiator, at 2,503,068 to 2,503,733
_2191
Pf1N1B4_2192 - Twin-arginine translocation protein TatB, at 2,503,926 to 2,505,059
_2192
Position (kb)
2503
2504 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.148 kb on + strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.149 kb on - strand, within Pf1N1B4_2189 at 2502.365 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.375 kb on + strand at 2502.384 kb on + strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.385 kb on - strand at 2502.395 kb on + strand at 2502.395 kb on + strand at 2502.422 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.450 kb on + strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.451 kb on - strand at 2502.510 kb on + strand at 2502.510 kb on + strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.511 kb on - strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.643 kb on + strand at 2502.643 kb on + strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.644 kb on - strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.724 kb on + strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.725 kb on - strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.751 kb on + strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.752 kb on - strand, within Pf1N1B4_2190 at 2502.789 kb on + strand, within Pf1N1B4_2190 at 2502.820 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.869 kb on + strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2502.870 kb on - strand, within Pf1N1B4_2190 at 2503.076 kb on + strand at 2503.076 kb on + strand at 2503.077 kb on - strand at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.151 kb on - strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.235 kb on + strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.236 kb on - strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.303 kb on + strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.304 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.372 kb on - strand, within Pf1N1B4_2191 at 2503.549 kb on + strand, within Pf1N1B4_2191 at 2503.549 kb on + strand, within Pf1N1B4_2191 at 2503.550 kb on - strand at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.550 kb on - strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.639 kb on + strand, within Pf1N1B4_2191 at 2503.714 kb on - strand at 2503.764 kb on + strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.765 kb on - strand at 2503.823 kb on - strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.837 kb on + strand at 2503.838 kb on - strand at 2503.888 kb on + strand at 2503.888 kb on + strand at 2503.888 kb on + strand at 2503.889 kb on - strand at 2503.891 kb on + strand at 2503.891 kb on + strand at 2503.891 kb on + strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.892 kb on - strand at 2503.920 kb on + strand at 2503.920 kb on + strand at 2503.921 kb on - strand at 2503.921 kb on - strand at 2503.921 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.949 kb on - strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.952 kb on + strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.953 kb on - strand at 2503.966 kb on + strand at 2503.966 kb on + strand at 2503.967 kb on - strand at 2503.967 kb on - strand at 2503.967 kb on - strand at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.226 kb on + strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.227 kb on - strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.290 kb on + strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.291 kb on - strand, within Pf1N1B4_2192 at 2504.562 kb on - strand, within Pf1N1B4_2192 at 2504.720 kb on + strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192 at 2504.721 kb on - strand, within Pf1N1B4_2192
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,502,148 + Pf1N1B4_2189 0.60 -1.9 2,502,148 + Pf1N1B4_2189 0.60 -0.6 2,502,149 - Pf1N1B4_2189 0.60 -0.6 2,502,149 - Pf1N1B4_2189 0.60 -2.2 2,502,149 - Pf1N1B4_2189 0.60 -0.0 2,502,365 + -1.0 2,502,375 + +0.4 2,502,375 + -0.6 2,502,375 + -0.6 2,502,384 + -1.8 2,502,385 - -1.6 2,502,385 - +0.1 2,502,385 - +1.0 2,502,395 + -0.0 2,502,395 + -0.4 2,502,422 + -0.2 2,502,450 + +0.7 2,502,450 + -0.6 2,502,450 + -0.1 2,502,450 + -1.2 2,502,451 - +0.4 2,502,451 - +0.1 2,502,451 - +0.4 2,502,451 - -1.2 2,502,451 - -1.2 2,502,451 - +1.0 2,502,451 - -0.0 2,502,451 - -0.2 2,502,451 - +2.3 2,502,510 + -0.1 2,502,510 + +0.0 2,502,511 - +0.1 2,502,511 - +1.7 2,502,511 - +0.4 2,502,511 - +1.4 2,502,643 + Pf1N1B4_2190 0.21 -0.2 2,502,643 + -1.2 2,502,643 + Pf1N1B4_2190 0.21 +0.6 2,502,644 - Pf1N1B4_2190 0.21 -0.3 2,502,644 - Pf1N1B4_2190 0.21 +0.8 2,502,644 - Pf1N1B4_2190 0.21 +0.4 2,502,724 + Pf1N1B4_2190 0.39 -1.6 2,502,724 + Pf1N1B4_2190 0.39 -1.2 2,502,724 + Pf1N1B4_2190 0.39 +2.4 2,502,725 - Pf1N1B4_2190 0.39 +0.9 2,502,725 - Pf1N1B4_2190 0.39 +0.4 2,502,751 + Pf1N1B4_2190 0.44 -1.2 2,502,751 + Pf1N1B4_2190 0.44 -0.9 2,502,751 + Pf1N1B4_2190 0.44 -0.2 2,502,751 + Pf1N1B4_2190 0.44 +0.4 2,502,752 - Pf1N1B4_2190 0.45 -1.2 2,502,752 - Pf1N1B4_2190 0.45 -0.5 2,502,752 - Pf1N1B4_2190 0.45 -0.3 2,502,789 + Pf1N1B4_2190 0.53 +1.1 2,502,820 + Pf1N1B4_2190 0.59 +1.0 2,502,869 + Pf1N1B4_2190 0.70 +0.8 2,502,869 + Pf1N1B4_2190 0.70 -0.5 2,502,869 + Pf1N1B4_2190 0.70 +0.2 2,502,869 + Pf1N1B4_2190 0.70 +0.4 2,502,869 + Pf1N1B4_2190 0.70 -1.3 2,502,869 + Pf1N1B4_2190 0.70 +0.4 2,502,869 + Pf1N1B4_2190 0.70 -1.4 2,502,869 + Pf1N1B4_2190 0.70 -1.6 2,502,869 + Pf1N1B4_2190 0.70 -0.7 2,502,869 + Pf1N1B4_2190 0.70 -0.2 2,502,869 + Pf1N1B4_2190 0.70 +0.7 2,502,869 + Pf1N1B4_2190 0.70 -0.2 2,502,869 + Pf1N1B4_2190 0.70 -1.0 2,502,869 + Pf1N1B4_2190 0.70 -1.6 2,502,870 - Pf1N1B4_2190 0.70 +1.4 2,502,870 - Pf1N1B4_2190 0.70 -0.3 2,502,870 - Pf1N1B4_2190 0.70 +1.0 2,502,870 - Pf1N1B4_2190 0.70 +0.4 2,503,076 + +1.8 2,503,076 + -0.6 2,503,077 - -0.2 2,503,151 - Pf1N1B4_2191 0.12 -0.3 2,503,151 - Pf1N1B4_2191 0.12 -2.4 2,503,235 + Pf1N1B4_2191 0.25 -1.2 2,503,235 + Pf1N1B4_2191 0.25 +2.0 2,503,235 + Pf1N1B4_2191 0.25 +2.2 2,503,236 - Pf1N1B4_2191 0.25 +1.7 2,503,236 - Pf1N1B4_2191 0.25 -0.2 2,503,303 + Pf1N1B4_2191 0.35 -0.2 2,503,303 + Pf1N1B4_2191 0.35 -0.5 2,503,303 + Pf1N1B4_2191 0.35 +1.4 2,503,303 + Pf1N1B4_2191 0.35 -0.2 2,503,303 + Pf1N1B4_2191 0.35 +1.4 2,503,303 + Pf1N1B4_2191 0.35 +1.7 2,503,303 + Pf1N1B4_2191 0.35 +0.6 2,503,303 + Pf1N1B4_2191 0.35 -2.4 2,503,303 + Pf1N1B4_2191 0.35 +0.4 2,503,303 + Pf1N1B4_2191 0.35 -0.0 2,503,303 + Pf1N1B4_2191 0.35 -0.5 2,503,303 + Pf1N1B4_2191 0.35 +0.7 2,503,303 + Pf1N1B4_2191 0.35 +2.4 2,503,303 + Pf1N1B4_2191 0.35 +0.1 2,503,304 - Pf1N1B4_2191 0.35 -0.6 2,503,304 - Pf1N1B4_2191 0.35 +0.4 2,503,304 - Pf1N1B4_2191 0.35 -0.2 2,503,304 - Pf1N1B4_2191 0.35 +0.6 2,503,304 - Pf1N1B4_2191 0.35 +0.4 2,503,304 - Pf1N1B4_2191 0.35 +2.7 2,503,304 - Pf1N1B4_2191 0.35 -0.6 2,503,304 - Pf1N1B4_2191 0.35 -1.2 2,503,304 - Pf1N1B4_2191 0.35 +0.9 2,503,304 - Pf1N1B4_2191 0.35 +1.7 2,503,304 - Pf1N1B4_2191 0.35 -1.2 2,503,304 - Pf1N1B4_2191 0.35 -0.6 2,503,304 - Pf1N1B4_2191 0.35 -0.1 2,503,372 - Pf1N1B4_2191 0.46 +0.2 2,503,372 - Pf1N1B4_2191 0.46 +1.7 2,503,372 - Pf1N1B4_2191 0.46 +1.4 2,503,549 + Pf1N1B4_2191 0.72 +0.8 2,503,549 + Pf1N1B4_2191 0.72 -1.6 2,503,550 - -0.6 2,503,550 - Pf1N1B4_2191 0.72 -0.6 2,503,550 - Pf1N1B4_2191 0.72 -0.2 2,503,550 - Pf1N1B4_2191 0.72 -1.2 2,503,639 + Pf1N1B4_2191 0.86 -1.4 2,503,639 + Pf1N1B4_2191 0.86 +0.4 2,503,639 + Pf1N1B4_2191 0.86 -0.6 2,503,714 - -0.3 2,503,764 + -0.0 2,503,765 - -1.6 2,503,765 - -0.6 2,503,765 - -0.6 2,503,765 - +2.0 2,503,765 - -0.9 2,503,823 - -1.3 2,503,837 + -0.9 2,503,837 + -1.1 2,503,837 + -0.1 2,503,837 + -1.6 2,503,838 - +1.4 2,503,888 + +1.0 2,503,888 + -0.3 2,503,888 + +0.4 2,503,889 - +0.4 2,503,891 + -0.2 2,503,891 + +1.0 2,503,891 + -0.1 2,503,892 - -1.2 2,503,892 - -1.2 2,503,892 - +0.8 2,503,892 - +1.0 2,503,920 + +0.7 2,503,920 + +0.4 2,503,921 - +0.7 2,503,921 - -0.9 2,503,921 - +2.0 2,503,949 - +0.4 2,503,949 - +0.4 2,503,949 - -0.2 2,503,949 - -0.3 2,503,949 - -0.6 2,503,949 - +1.0 2,503,952 + -0.6 2,503,952 + +1.7 2,503,952 + +0.8 2,503,952 + +0.4 2,503,953 - -1.8 2,503,953 - -0.1 2,503,953 - -0.8 2,503,953 - -0.1 2,503,953 - +0.4 2,503,966 + -0.6 2,503,966 + -0.6 2,503,967 - -0.6 2,503,967 - +1.4 2,503,967 - +2.0 2,504,226 + Pf1N1B4_2192 0.26 -0.6 2,504,226 + Pf1N1B4_2192 0.26 -0.1 2,504,226 + Pf1N1B4_2192 0.26 +1.1 2,504,226 + Pf1N1B4_2192 0.26 +0.1 2,504,226 + Pf1N1B4_2192 0.26 -0.0 2,504,226 + Pf1N1B4_2192 0.26 +0.2 2,504,227 - Pf1N1B4_2192 0.27 -0.1 2,504,227 - Pf1N1B4_2192 0.27 +0.9 2,504,227 - Pf1N1B4_2192 0.27 -0.3 2,504,290 + Pf1N1B4_2192 0.32 -1.2 2,504,290 + Pf1N1B4_2192 0.32 +0.4 2,504,290 + Pf1N1B4_2192 0.32 -0.6 2,504,290 + Pf1N1B4_2192 0.32 -0.6 2,504,290 + Pf1N1B4_2192 0.32 +0.4 2,504,291 - Pf1N1B4_2192 0.32 -1.2 2,504,291 - Pf1N1B4_2192 0.32 -1.2 2,504,291 - Pf1N1B4_2192 0.32 -1.9 2,504,291 - Pf1N1B4_2192 0.32 +0.8 2,504,562 - Pf1N1B4_2192 0.56 +0.4 2,504,720 + Pf1N1B4_2192 0.70 +0.4 2,504,721 - Pf1N1B4_2192 0.70 +2.3 2,504,721 - Pf1N1B4_2192 0.70 -1.6
Or see this region's nucleotide sequence