Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1970 and Pf1N1B4_1971 overlap by 7 nucleotides Pf1N1B4_1971 and Pf1N1B4_1972 are separated by 65 nucleotides
Pf1N1B4_1970 - O-antigen acetylase, at 2,252,088 to 2,253,236
_1970
Pf1N1B4_1971 - SAM-dependent methyltransferases, at 2,253,230 to 2,254,066
_1971
Pf1N1B4_1972 - FIG01055313: hypothetical protein, at 2,254,132 to 2,255,325
_1972
Position (kb)
2253
2254
2255 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2252.300 kb on + strand, within Pf1N1B4_1970 at 2252.313 kb on + strand, within Pf1N1B4_1970 at 2252.314 kb on - strand, within Pf1N1B4_1970 at 2252.407 kb on + strand, within Pf1N1B4_1970 at 2252.407 kb on + strand, within Pf1N1B4_1970 at 2252.408 kb on - strand, within Pf1N1B4_1970 at 2252.408 kb on - strand, within Pf1N1B4_1970 at 2252.481 kb on + strand, within Pf1N1B4_1970 at 2252.481 kb on + strand, within Pf1N1B4_1970 at 2252.482 kb on - strand, within Pf1N1B4_1970 at 2252.486 kb on + strand, within Pf1N1B4_1970 at 2252.487 kb on - strand, within Pf1N1B4_1970 at 2252.541 kb on + strand, within Pf1N1B4_1970 at 2252.571 kb on + strand, within Pf1N1B4_1970 at 2252.571 kb on + strand, within Pf1N1B4_1970 at 2252.572 kb on - strand, within Pf1N1B4_1970 at 2252.572 kb on - strand, within Pf1N1B4_1970 at 2252.572 kb on - strand, within Pf1N1B4_1970 at 2252.572 kb on - strand, within Pf1N1B4_1970 at 2252.572 kb on - strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.575 kb on + strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.576 kb on - strand, within Pf1N1B4_1970 at 2252.610 kb on + strand, within Pf1N1B4_1970 at 2252.610 kb on + strand, within Pf1N1B4_1970 at 2252.610 kb on + strand, within Pf1N1B4_1970 at 2252.611 kb on - strand at 2252.650 kb on + strand, within Pf1N1B4_1970 at 2252.650 kb on + strand, within Pf1N1B4_1970 at 2252.651 kb on - strand, within Pf1N1B4_1970 at 2252.653 kb on + strand, within Pf1N1B4_1970 at 2252.653 kb on + strand, within Pf1N1B4_1970 at 2252.822 kb on + strand, within Pf1N1B4_1970 at 2252.822 kb on + strand, within Pf1N1B4_1970 at 2252.822 kb on + strand, within Pf1N1B4_1970 at 2252.822 kb on + strand, within Pf1N1B4_1970 at 2252.823 kb on - strand, within Pf1N1B4_1970 at 2252.823 kb on - strand, within Pf1N1B4_1970 at 2252.823 kb on - strand, within Pf1N1B4_1970 at 2252.910 kb on + strand, within Pf1N1B4_1970 at 2252.910 kb on + strand, within Pf1N1B4_1970 at 2252.911 kb on - strand, within Pf1N1B4_1970 at 2252.911 kb on - strand, within Pf1N1B4_1970 at 2252.911 kb on - strand, within Pf1N1B4_1970 at 2252.922 kb on + strand, within Pf1N1B4_1970 at 2252.922 kb on + strand, within Pf1N1B4_1970 at 2252.979 kb on + strand, within Pf1N1B4_1970 at 2252.988 kb on + strand, within Pf1N1B4_1970 at 2252.988 kb on + strand, within Pf1N1B4_1970 at 2252.988 kb on + strand, within Pf1N1B4_1970 at 2253.009 kb on + strand, within Pf1N1B4_1970 at 2253.115 kb on + strand, within Pf1N1B4_1970 at 2253.115 kb on + strand, within Pf1N1B4_1970 at 2253.115 kb on + strand, within Pf1N1B4_1970 at 2253.115 kb on + strand, within Pf1N1B4_1970 at 2253.132 kb on + strand at 2253.224 kb on + strand at 2253.224 kb on + strand at 2253.225 kb on - strand at 2253.225 kb on - strand at 2253.225 kb on - strand at 2253.225 kb on - strand at 2253.374 kb on - strand, within Pf1N1B4_1971 at 2253.701 kb on + strand, within Pf1N1B4_1971 at 2253.701 kb on + strand, within Pf1N1B4_1971 at 2253.701 kb on + strand, within Pf1N1B4_1971 at 2253.702 kb on - strand, within Pf1N1B4_1971 at 2253.702 kb on - strand, within Pf1N1B4_1971 at 2253.702 kb on - strand, within Pf1N1B4_1971 at 2253.764 kb on + strand, within Pf1N1B4_1971 at 2253.764 kb on + strand, within Pf1N1B4_1971 at 2253.860 kb on + strand, within Pf1N1B4_1971 at 2253.869 kb on + strand, within Pf1N1B4_1971 at 2253.869 kb on + strand, within Pf1N1B4_1971 at 2253.869 kb on + strand, within Pf1N1B4_1971 at 2253.870 kb on - strand, within Pf1N1B4_1971 at 2253.870 kb on - strand, within Pf1N1B4_1971 at 2253.998 kb on - strand at 2253.998 kb on - strand at 2253.998 kb on - strand at 2254.111 kb on - strand at 2254.225 kb on + strand at 2254.225 kb on + strand at 2254.225 kb on + strand at 2254.225 kb on + strand at 2254.225 kb on + strand at 2254.226 kb on - strand at 2254.226 kb on - strand at 2254.226 kb on - strand at 2254.226 kb on - strand at 2254.226 kb on - strand at 2254.305 kb on - strand, within Pf1N1B4_1972 at 2254.568 kb on + strand, within Pf1N1B4_1972 at 2254.568 kb on + strand, within Pf1N1B4_1972 at 2254.673 kb on + strand, within Pf1N1B4_1972 at 2254.673 kb on + strand, within Pf1N1B4_1972 at 2254.674 kb on - strand, within Pf1N1B4_1972 at 2254.674 kb on - strand, within Pf1N1B4_1972 at 2254.674 kb on - strand, within Pf1N1B4_1972 at 2254.674 kb on - strand, within Pf1N1B4_1972 at 2254.759 kb on - strand, within Pf1N1B4_1972 at 2254.777 kb on + strand, within Pf1N1B4_1972 at 2254.777 kb on + strand, within Pf1N1B4_1972 at 2254.777 kb on + strand, within Pf1N1B4_1972 at 2254.778 kb on - strand, within Pf1N1B4_1972 at 2254.884 kb on - strand, within Pf1N1B4_1972 at 2255.015 kb on + strand, within Pf1N1B4_1972 at 2255.037 kb on + strand, within Pf1N1B4_1972
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,252,300 + Pf1N1B4_1970 0.18 +0.6 2,252,313 + Pf1N1B4_1970 0.20 -0.1 2,252,314 - Pf1N1B4_1970 0.20 -0.5 2,252,407 + Pf1N1B4_1970 0.28 -0.5 2,252,407 + Pf1N1B4_1970 0.28 -1.9 2,252,408 - Pf1N1B4_1970 0.28 -0.5 2,252,408 - Pf1N1B4_1970 0.28 -0.9 2,252,481 + Pf1N1B4_1970 0.34 +0.5 2,252,481 + Pf1N1B4_1970 0.34 +1.1 2,252,482 - Pf1N1B4_1970 0.34 -0.1 2,252,486 + Pf1N1B4_1970 0.35 +0.0 2,252,487 - Pf1N1B4_1970 0.35 -1.9 2,252,541 + Pf1N1B4_1970 0.39 -1.5 2,252,571 + Pf1N1B4_1970 0.42 -0.1 2,252,571 + Pf1N1B4_1970 0.42 +0.2 2,252,572 - Pf1N1B4_1970 0.42 +2.3 2,252,572 - Pf1N1B4_1970 0.42 +0.5 2,252,572 - Pf1N1B4_1970 0.42 +0.0 2,252,572 - Pf1N1B4_1970 0.42 +1.2 2,252,572 - Pf1N1B4_1970 0.42 -1.5 2,252,575 + Pf1N1B4_1970 0.42 +0.8 2,252,575 + Pf1N1B4_1970 0.42 -0.1 2,252,575 + Pf1N1B4_1970 0.42 -1.0 2,252,575 + Pf1N1B4_1970 0.42 +2.5 2,252,575 + Pf1N1B4_1970 0.42 -0.3 2,252,575 + Pf1N1B4_1970 0.42 -0.1 2,252,575 + Pf1N1B4_1970 0.42 +1.0 2,252,576 - Pf1N1B4_1970 0.42 -1.9 2,252,576 - Pf1N1B4_1970 0.42 +0.5 2,252,576 - Pf1N1B4_1970 0.42 +0.9 2,252,576 - Pf1N1B4_1970 0.42 -1.1 2,252,576 - Pf1N1B4_1970 0.42 -0.1 2,252,576 - Pf1N1B4_1970 0.42 +0.9 2,252,576 - Pf1N1B4_1970 0.42 -0.1 2,252,610 + Pf1N1B4_1970 0.45 -0.8 2,252,610 + Pf1N1B4_1970 0.45 +0.4 2,252,610 + Pf1N1B4_1970 0.45 -0.5 2,252,611 - -1.5 2,252,650 + Pf1N1B4_1970 0.49 -0.3 2,252,650 + Pf1N1B4_1970 0.49 -2.2 2,252,651 - Pf1N1B4_1970 0.49 -0.7 2,252,653 + Pf1N1B4_1970 0.49 +1.0 2,252,653 + Pf1N1B4_1970 0.49 -1.4 2,252,822 + Pf1N1B4_1970 0.64 -1.1 2,252,822 + Pf1N1B4_1970 0.64 -1.7 2,252,822 + Pf1N1B4_1970 0.64 -0.5 2,252,822 + Pf1N1B4_1970 0.64 +1.1 2,252,823 - Pf1N1B4_1970 0.64 -0.5 2,252,823 - Pf1N1B4_1970 0.64 +0.5 2,252,823 - Pf1N1B4_1970 0.64 -0.5 2,252,910 + Pf1N1B4_1970 0.72 +0.2 2,252,910 + Pf1N1B4_1970 0.72 +0.8 2,252,911 - Pf1N1B4_1970 0.72 -0.1 2,252,911 - Pf1N1B4_1970 0.72 +0.0 2,252,911 - Pf1N1B4_1970 0.72 -1.1 2,252,922 + Pf1N1B4_1970 0.73 -0.3 2,252,922 + Pf1N1B4_1970 0.73 +1.5 2,252,979 + Pf1N1B4_1970 0.78 -0.3 2,252,988 + Pf1N1B4_1970 0.78 -1.9 2,252,988 + Pf1N1B4_1970 0.78 -0.1 2,252,988 + Pf1N1B4_1970 0.78 -0.2 2,253,009 + Pf1N1B4_1970 0.80 +0.8 2,253,115 + Pf1N1B4_1970 0.89 +0.9 2,253,115 + Pf1N1B4_1970 0.89 -0.1 2,253,115 + Pf1N1B4_1970 0.89 -2.5 2,253,115 + Pf1N1B4_1970 0.89 -2.4 2,253,132 + +1.5 2,253,224 + -0.5 2,253,224 + +1.7 2,253,225 - +0.9 2,253,225 - -1.1 2,253,225 - -0.1 2,253,225 - +0.5 2,253,374 - Pf1N1B4_1971 0.17 -1.1 2,253,701 + Pf1N1B4_1971 0.56 -2.1 2,253,701 + Pf1N1B4_1971 0.56 -2.0 2,253,701 + Pf1N1B4_1971 0.56 -1.1 2,253,702 - Pf1N1B4_1971 0.56 -0.5 2,253,702 - Pf1N1B4_1971 0.56 +0.1 2,253,702 - Pf1N1B4_1971 0.56 +0.0 2,253,764 + Pf1N1B4_1971 0.64 -1.7 2,253,764 + Pf1N1B4_1971 0.64 -0.1 2,253,860 + Pf1N1B4_1971 0.75 -0.5 2,253,869 + Pf1N1B4_1971 0.76 -0.5 2,253,869 + Pf1N1B4_1971 0.76 -0.8 2,253,869 + Pf1N1B4_1971 0.76 -0.5 2,253,870 - Pf1N1B4_1971 0.76 -0.1 2,253,870 - Pf1N1B4_1971 0.76 -0.9 2,253,998 - -2.4 2,253,998 - +1.0 2,253,998 - -1.5 2,254,111 - +0.3 2,254,225 + +0.5 2,254,225 + -1.1 2,254,225 + -0.5 2,254,225 + +1.0 2,254,225 + -1.1 2,254,226 - -1.5 2,254,226 - +1.0 2,254,226 - +1.0 2,254,226 - -0.5 2,254,226 - -1.1 2,254,305 - Pf1N1B4_1972 0.14 -0.5 2,254,568 + Pf1N1B4_1972 0.37 +0.5 2,254,568 + Pf1N1B4_1972 0.37 -1.1 2,254,673 + Pf1N1B4_1972 0.45 -0.5 2,254,673 + Pf1N1B4_1972 0.45 +2.5 2,254,674 - Pf1N1B4_1972 0.45 -0.1 2,254,674 - Pf1N1B4_1972 0.45 -0.4 2,254,674 - Pf1N1B4_1972 0.45 +0.0 2,254,674 - Pf1N1B4_1972 0.45 -1.1 2,254,759 - Pf1N1B4_1972 0.53 -0.5 2,254,777 + Pf1N1B4_1972 0.54 -0.3 2,254,777 + Pf1N1B4_1972 0.54 +0.0 2,254,777 + Pf1N1B4_1972 0.54 +1.5 2,254,778 - Pf1N1B4_1972 0.54 -0.3 2,254,884 - Pf1N1B4_1972 0.63 +0.3 2,255,015 + Pf1N1B4_1972 0.74 +0.5 2,255,037 + Pf1N1B4_1972 0.76 -1.1
Or see this region's nucleotide sequence