Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1769 and Pf1N1B4_1770 are separated by 268 nucleotides Pf1N1B4_1770 and Pf1N1B4_1771 overlap by 1 nucleotides
Pf1N1B4_1769 - Glutamate--cysteine ligase (EC 6.3.2.2), at 2,021,931 to 2,023,514
_1769
Pf1N1B4_1770 - Uncharacterized protein, possibly involved in aromatic compounds catabolism, at 2,023,783 to 2,024,166
_1770
Pf1N1B4_1771 - Transcription accessory protein (S1 RNA-binding domain), at 2,024,166 to 2,026,490
_1771
Position (kb)
2023
2024
2025 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2023.123 kb on + strand, within Pf1N1B4_1769 at 2023.124 kb on - strand, within Pf1N1B4_1769 at 2023.568 kb on + strand at 2023.568 kb on + strand at 2023.569 kb on - strand at 2023.582 kb on + strand at 2023.682 kb on + strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.697 kb on - strand at 2023.749 kb on - strand at 2023.776 kb on + strand at 2023.776 kb on + strand at 2023.777 kb on - strand at 2023.777 kb on - strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.780 kb on + strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.781 kb on - strand at 2023.784 kb on + strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.785 kb on - strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.925 kb on + strand at 2023.925 kb on + strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2023.926 kb on - strand, within Pf1N1B4_1770 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.459 kb on + strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand, within Pf1N1B4_1771 at 2024.460 kb on - strand at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.463 kb on + strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.464 kb on - strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.683 kb on + strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.684 kb on - strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2024.829 kb on + strand, within Pf1N1B4_1771 at 2025.089 kb on - strand, within Pf1N1B4_1771
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 2,023,123 + Pf1N1B4_1769 0.75 +1.2 2,023,124 - Pf1N1B4_1769 0.75 +0.4 2,023,568 + +1.2 2,023,568 + +0.4 2,023,569 - +1.6 2,023,582 + -1.6 2,023,682 + +0.4 2,023,697 - -0.3 2,023,697 - -1.2 2,023,697 - +0.8 2,023,697 - -0.7 2,023,749 - -0.3 2,023,776 + +0.4 2,023,776 + -0.6 2,023,777 - -0.8 2,023,777 - -0.3 2,023,780 + +1.4 2,023,780 + -0.9 2,023,780 + +0.4 2,023,780 + +1.0 2,023,780 + +1.0 2,023,780 + +0.4 2,023,781 - +3.0 2,023,781 - +0.7 2,023,781 - -1.2 2,023,781 - +0.8 2,023,781 - +0.8 2,023,784 + -0.6 2,023,785 - -1.2 2,023,785 - +0.8 2,023,785 - -0.6 2,023,785 - +0.4 2,023,785 - -1.4 2,023,785 - -1.4 2,023,925 + Pf1N1B4_1770 0.37 +1.4 2,023,925 + Pf1N1B4_1770 0.37 -1.6 2,023,925 + Pf1N1B4_1770 0.37 +1.2 2,023,925 + Pf1N1B4_1770 0.37 +0.1 2,023,925 + Pf1N1B4_1770 0.37 -3.0 2,023,925 + Pf1N1B4_1770 0.37 +0.0 2,023,925 + -0.6 2,023,925 + Pf1N1B4_1770 0.37 +0.4 2,023,926 - Pf1N1B4_1770 0.37 +0.4 2,023,926 - Pf1N1B4_1770 0.37 -1.6 2,023,926 - Pf1N1B4_1770 0.37 -2.2 2,023,926 - Pf1N1B4_1770 0.37 -1.2 2,023,926 - Pf1N1B4_1770 0.37 -0.9 2,023,926 - Pf1N1B4_1770 0.37 -0.6 2,023,926 - Pf1N1B4_1770 0.37 -0.8 2,024,459 + Pf1N1B4_1771 0.13 +0.1 2,024,459 + Pf1N1B4_1771 0.13 -0.2 2,024,459 + Pf1N1B4_1771 0.13 +0.8 2,024,459 + Pf1N1B4_1771 0.13 -0.9 2,024,459 + Pf1N1B4_1771 0.13 -0.6 2,024,459 + Pf1N1B4_1771 0.13 -0.6 2,024,459 + Pf1N1B4_1771 0.13 +0.4 2,024,459 + Pf1N1B4_1771 0.13 -2.6 2,024,459 + Pf1N1B4_1771 0.13 +0.4 2,024,459 + Pf1N1B4_1771 0.13 -0.8 2,024,459 + Pf1N1B4_1771 0.13 +0.4 2,024,459 + Pf1N1B4_1771 0.13 -0.4 2,024,459 + Pf1N1B4_1771 0.13 +2.7 2,024,459 + Pf1N1B4_1771 0.13 +1.0 2,024,460 - Pf1N1B4_1771 0.13 +1.0 2,024,460 - Pf1N1B4_1771 0.13 -1.4 2,024,460 - Pf1N1B4_1771 0.13 -1.2 2,024,460 - Pf1N1B4_1771 0.13 -2.2 2,024,460 - Pf1N1B4_1771 0.13 +0.4 2,024,460 - Pf1N1B4_1771 0.13 -0.3 2,024,460 - Pf1N1B4_1771 0.13 +1.2 2,024,460 - Pf1N1B4_1771 0.13 -1.6 2,024,460 - Pf1N1B4_1771 0.13 -0.4 2,024,460 - +0.4 2,024,460 - Pf1N1B4_1771 0.13 -1.2 2,024,460 - -0.6 2,024,460 - Pf1N1B4_1771 0.13 +2.0 2,024,460 - Pf1N1B4_1771 0.13 -0.6 2,024,460 - Pf1N1B4_1771 0.13 +1.4 2,024,460 - Pf1N1B4_1771 0.13 +0.4 2,024,460 - +0.4 2,024,463 + Pf1N1B4_1771 0.13 -0.3 2,024,463 + Pf1N1B4_1771 0.13 -0.9 2,024,463 + Pf1N1B4_1771 0.13 -0.3 2,024,463 + Pf1N1B4_1771 0.13 +0.4 2,024,463 + Pf1N1B4_1771 0.13 +0.2 2,024,464 - Pf1N1B4_1771 0.13 -2.2 2,024,464 - Pf1N1B4_1771 0.13 -1.2 2,024,464 - Pf1N1B4_1771 0.13 -0.8 2,024,683 + Pf1N1B4_1771 0.22 -1.2 2,024,683 + Pf1N1B4_1771 0.22 -0.8 2,024,683 + Pf1N1B4_1771 0.22 +0.4 2,024,683 + Pf1N1B4_1771 0.22 -1.3 2,024,683 + Pf1N1B4_1771 0.22 +1.0 2,024,684 - Pf1N1B4_1771 0.22 +1.2 2,024,684 - Pf1N1B4_1771 0.22 +0.9 2,024,684 - Pf1N1B4_1771 0.22 -1.6 2,024,829 + Pf1N1B4_1771 0.29 +0.4 2,024,829 + Pf1N1B4_1771 0.29 +1.0 2,024,829 + Pf1N1B4_1771 0.29 +0.4 2,025,089 - Pf1N1B4_1771 0.40 +2.1
Or see this region's nucleotide sequence