Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1666 and Pf1N1B4_1667 are separated by 40 nucleotides Pf1N1B4_1667 and Pf1N1B4_1668 are separated by 117 nucleotides Pf1N1B4_1668 and Pf1N1B4_1669 overlap by 4 nucleotides
Pf1N1B4_1666 - C4-dicarboxylate transport transcriptional regulatory protein, at 1,910,422 to 1,911,816
_1666
Pf1N1B4_1667 - Histone acetyltransferase HPA2 and related acetyltransferases, at 1,911,857 to 1,912,315
_1667
Pf1N1B4_1668 - 3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7), at 1,912,433 to 1,913,266
_1668
Pf1N1B4_1669 - ADP compounds hydrolase NudE (EC 3.6.1.-), at 1,913,263 to 1,913,829
_1669
Position (kb)
1911
1912
1913 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1911.337 kb on + strand, within Pf1N1B4_1666 at 1911.337 kb on + strand, within Pf1N1B4_1666 at 1911.337 kb on + strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.338 kb on - strand, within Pf1N1B4_1666 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.515 kb on + strand, within Pf1N1B4_1666 at 1911.516 kb on - strand, within Pf1N1B4_1666 at 1911.819 kb on + strand at 1911.825 kb on - strand at 1912.022 kb on + strand, within Pf1N1B4_1667 at 1912.023 kb on - strand, within Pf1N1B4_1667 at 1912.023 kb on - strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.208 kb on + strand, within Pf1N1B4_1667 at 1912.209 kb on - strand at 1912.209 kb on - strand, within Pf1N1B4_1667 at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.298 kb on + strand at 1912.299 kb on - strand at 1912.299 kb on - strand at 1912.299 kb on - strand at 1912.422 kb on - strand at 1912.422 kb on - strand at 1912.422 kb on - strand at 1912.731 kb on - strand, within Pf1N1B4_1668 at 1912.837 kb on + strand, within Pf1N1B4_1668 at 1912.838 kb on - strand, within Pf1N1B4_1668 at 1912.875 kb on + strand, within Pf1N1B4_1668 at 1912.875 kb on + strand, within Pf1N1B4_1668 at 1912.876 kb on - strand, within Pf1N1B4_1668 at 1912.876 kb on - strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.972 kb on + strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1912.973 kb on - strand, within Pf1N1B4_1668 at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.293 kb on + strand at 1913.294 kb on - strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.309 kb on + strand at 1913.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 1,911,337 + Pf1N1B4_1666 0.66 -0.6 1,911,337 + Pf1N1B4_1666 0.66 -0.3 1,911,337 + Pf1N1B4_1666 0.66 -2.2 1,911,338 - Pf1N1B4_1666 0.66 +1.4 1,911,338 - Pf1N1B4_1666 0.66 +2.4 1,911,338 - Pf1N1B4_1666 0.66 -0.6 1,911,338 - Pf1N1B4_1666 0.66 +1.0 1,911,338 - Pf1N1B4_1666 0.66 +1.6 1,911,338 - Pf1N1B4_1666 0.66 +1.6 1,911,338 - Pf1N1B4_1666 0.66 +0.4 1,911,338 - Pf1N1B4_1666 0.66 -0.6 1,911,338 - Pf1N1B4_1666 0.66 -1.8 1,911,515 + Pf1N1B4_1666 0.78 -1.2 1,911,515 + Pf1N1B4_1666 0.78 -2.2 1,911,515 + Pf1N1B4_1666 0.78 +0.1 1,911,516 - Pf1N1B4_1666 0.78 +1.4 1,911,819 + +0.4 1,911,825 - -1.2 1,912,022 + Pf1N1B4_1667 0.36 -2.8 1,912,023 - Pf1N1B4_1667 0.36 -1.2 1,912,023 - Pf1N1B4_1667 0.36 -1.2 1,912,208 + Pf1N1B4_1667 0.76 -0.6 1,912,208 + Pf1N1B4_1667 0.76 -1.9 1,912,208 + Pf1N1B4_1667 0.76 -0.9 1,912,208 + Pf1N1B4_1667 0.76 -1.4 1,912,208 + Pf1N1B4_1667 0.76 +0.5 1,912,209 - +1.0 1,912,209 - Pf1N1B4_1667 0.77 -1.2 1,912,298 + -0.6 1,912,298 + +0.4 1,912,298 + +1.4 1,912,298 + -1.6 1,912,298 + -1.9 1,912,298 + -1.2 1,912,298 + +0.3 1,912,298 + -0.4 1,912,298 + +0.8 1,912,298 + +2.4 1,912,298 + +0.4 1,912,298 + -0.2 1,912,298 + -0.4 1,912,299 - +1.4 1,912,299 - +1.4 1,912,299 - +1.1 1,912,422 - -0.2 1,912,422 - -1.6 1,912,422 - -0.2 1,912,731 - Pf1N1B4_1668 0.36 -0.6 1,912,837 + Pf1N1B4_1668 0.48 -1.6 1,912,838 - Pf1N1B4_1668 0.49 +0.4 1,912,875 + Pf1N1B4_1668 0.53 -0.8 1,912,875 + Pf1N1B4_1668 0.53 -0.4 1,912,876 - Pf1N1B4_1668 0.53 -1.6 1,912,876 - Pf1N1B4_1668 0.53 -3.4 1,912,972 + Pf1N1B4_1668 0.65 +0.1 1,912,972 + Pf1N1B4_1668 0.65 -3.0 1,912,972 + Pf1N1B4_1668 0.65 -2.2 1,912,972 + Pf1N1B4_1668 0.65 +0.4 1,912,972 + Pf1N1B4_1668 0.65 -1.2 1,912,973 - Pf1N1B4_1668 0.65 -1.0 1,912,973 - Pf1N1B4_1668 0.65 +1.0 1,912,973 - Pf1N1B4_1668 0.65 -2.8 1,912,973 - Pf1N1B4_1668 0.65 -1.9 1,912,973 - Pf1N1B4_1668 0.65 -0.4 1,913,293 + -1.2 1,913,293 + -0.0 1,913,293 + +0.7 1,913,293 + -0.1 1,913,293 + -1.2 1,913,293 + -0.4 1,913,294 - +0.2 1,913,309 + -0.6 1,913,309 + -1.3 1,913,309 + +1.4 1,913,309 + -0.3 1,913,309 + +0.4 1,913,310 - -0.0
Or see this region's nucleotide sequence