Experiment: D-Cycloserine 0.1 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1568 and Pf1N1B4_1569 overlap by 4 nucleotides Pf1N1B4_1569 and Pf1N1B4_1570 overlap by 4 nucleotides Pf1N1B4_1570 and Pf1N1B4_1571 are separated by 219 nucleotides
Pf1N1B4_1568 - Twin-arginine translocation protein TatB, at 1,804,711 to 1,805,136
_1568
Pf1N1B4_1569 - Twin-arginine translocation protein TatC, at 1,805,133 to 1,805,927
_1569
Pf1N1B4_1570 - Ribosomal RNA small subunit methyltransferase E (EC 2.1.1.-), at 1,805,924 to 1,806,631
_1570
Pf1N1B4_1571 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 1,806,851 to 1,808,767
_1571
Position (kb)
1805
1806
1807 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1804.986 kb on - strand, within Pf1N1B4_1568 at 1805.060 kb on + strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.061 kb on - strand, within Pf1N1B4_1568 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.268 kb on + strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand at 1805.269 kb on - strand at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.269 kb on - strand, within Pf1N1B4_1569 at 1805.298 kb on + strand, within Pf1N1B4_1569 at 1805.298 kb on + strand, within Pf1N1B4_1569 at 1805.299 kb on - strand, within Pf1N1B4_1569 at 1805.299 kb on - strand, within Pf1N1B4_1569 at 1805.299 kb on - strand, within Pf1N1B4_1569 at 1805.299 kb on - strand, within Pf1N1B4_1569 at 1805.299 kb on - strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.442 kb on + strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.443 kb on - strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.499 kb on + strand, within Pf1N1B4_1569 at 1805.500 kb on - strand at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.500 kb on - strand, within Pf1N1B4_1569 at 1805.520 kb on + strand, within Pf1N1B4_1569 at 1805.520 kb on + strand, within Pf1N1B4_1569 at 1805.520 kb on + strand, within Pf1N1B4_1569 at 1805.520 kb on + strand, within Pf1N1B4_1569 at 1805.521 kb on - strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.604 kb on + strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.605 kb on - strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.706 kb on + strand, within Pf1N1B4_1569 at 1805.707 kb on - strand, within Pf1N1B4_1569 at 1805.727 kb on + strand, within Pf1N1B4_1569 at 1805.727 kb on + strand, within Pf1N1B4_1569 at 1805.727 kb on + strand, within Pf1N1B4_1569 at 1805.727 kb on + strand, within Pf1N1B4_1569 at 1805.727 kb on + strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1805.728 kb on - strand, within Pf1N1B4_1569 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.081 kb on + strand, within Pf1N1B4_1570 at 1806.082 kb on - strand, within Pf1N1B4_1570 at 1806.082 kb on - strand at 1806.154 kb on - strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.452 kb on + strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.453 kb on - strand, within Pf1N1B4_1570 at 1806.645 kb on - strand at 1806.717 kb on + strand at 1806.775 kb on + strand at 1806.784 kb on - strand at 1806.784 kb on - strand at 1806.818 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.828 kb on + strand at 1806.829 kb on - strand at 1806.844 kb on + strand at 1806.844 kb on + strand at 1806.845 kb on - strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.854 kb on + strand at 1806.855 kb on - strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.885 kb on + strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1806.886 kb on - strand at 1807.007 kb on - strand at 1807.058 kb on + strand, within Pf1N1B4_1571 at 1807.059 kb on - strand, within Pf1N1B4_1571 at 1807.206 kb on - strand, within Pf1N1B4_1571 at 1807.225 kb on + strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.226 kb on - strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.415 kb on + strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.416 kb on - strand, within Pf1N1B4_1571 at 1807.418 kb on - strand, within Pf1N1B4_1571
Per-strain Table
Position Strand Gene LocusTag Fraction D-Cycloserine 0.1 mg/ml remove 1,804,986 - Pf1N1B4_1568 0.65 -1.3 1,805,060 + Pf1N1B4_1568 0.82 -2.0 1,805,061 - Pf1N1B4_1568 0.82 -1.5 1,805,061 - Pf1N1B4_1568 0.82 -1.2 1,805,061 - Pf1N1B4_1568 0.82 -0.5 1,805,061 - Pf1N1B4_1568 0.82 -1.0 1,805,061 - Pf1N1B4_1568 0.82 -1.2 1,805,061 - Pf1N1B4_1568 0.82 -2.6 1,805,061 - Pf1N1B4_1568 0.82 +1.6 1,805,268 + Pf1N1B4_1569 0.17 +0.0 1,805,268 + Pf1N1B4_1569 0.17 +0.4 1,805,268 + +1.4 1,805,268 + Pf1N1B4_1569 0.17 +2.4 1,805,268 + Pf1N1B4_1569 0.17 +0.5 1,805,268 + Pf1N1B4_1569 0.17 -0.6 1,805,268 + Pf1N1B4_1569 0.17 -1.2 1,805,268 + Pf1N1B4_1569 0.17 +1.2 1,805,268 + Pf1N1B4_1569 0.17 +0.1 1,805,268 + Pf1N1B4_1569 0.17 +1.4 1,805,268 + Pf1N1B4_1569 0.17 -1.2 1,805,268 + Pf1N1B4_1569 0.17 -0.2 1,805,268 + Pf1N1B4_1569 0.17 +0.0 1,805,268 + Pf1N1B4_1569 0.17 -0.2 1,805,268 + Pf1N1B4_1569 0.17 -2.5 1,805,268 + Pf1N1B4_1569 0.17 +0.1 1,805,268 + Pf1N1B4_1569 0.17 -0.5 1,805,268 + Pf1N1B4_1569 0.17 -0.6 1,805,268 + Pf1N1B4_1569 0.17 +1.0 1,805,268 + Pf1N1B4_1569 0.17 +0.2 1,805,268 + Pf1N1B4_1569 0.17 -1.2 1,805,269 - Pf1N1B4_1569 0.17 -0.9 1,805,269 - Pf1N1B4_1569 0.17 -1.9 1,805,269 - Pf1N1B4_1569 0.17 +0.4 1,805,269 - Pf1N1B4_1569 0.17 +1.4 1,805,269 - Pf1N1B4_1569 0.17 -2.5 1,805,269 - Pf1N1B4_1569 0.17 -1.8 1,805,269 - Pf1N1B4_1569 0.17 +0.4 1,805,269 - Pf1N1B4_1569 0.17 -0.2 1,805,269 - -0.6 1,805,269 - +1.4 1,805,269 - Pf1N1B4_1569 0.17 -0.1 1,805,269 - Pf1N1B4_1569 0.17 -2.6 1,805,269 - Pf1N1B4_1569 0.17 -1.1 1,805,298 + Pf1N1B4_1569 0.21 -0.6 1,805,298 + Pf1N1B4_1569 0.21 +1.4 1,805,299 - Pf1N1B4_1569 0.21 -2.2 1,805,299 - Pf1N1B4_1569 0.21 +1.5 1,805,299 - Pf1N1B4_1569 0.21 -0.5 1,805,299 - Pf1N1B4_1569 0.21 -0.2 1,805,299 - Pf1N1B4_1569 0.21 -1.8 1,805,442 + Pf1N1B4_1569 0.39 -0.2 1,805,442 + Pf1N1B4_1569 0.39 -2.8 1,805,442 + Pf1N1B4_1569 0.39 -1.2 1,805,442 + Pf1N1B4_1569 0.39 -0.2 1,805,442 + Pf1N1B4_1569 0.39 -1.6 1,805,442 + Pf1N1B4_1569 0.39 -2.0 1,805,442 + Pf1N1B4_1569 0.39 +0.6 1,805,442 + Pf1N1B4_1569 0.39 -0.5 1,805,442 + Pf1N1B4_1569 0.39 -1.2 1,805,442 + Pf1N1B4_1569 0.39 +0.4 1,805,442 + Pf1N1B4_1569 0.39 -0.2 1,805,442 + Pf1N1B4_1569 0.39 +0.4 1,805,443 - Pf1N1B4_1569 0.39 -1.1 1,805,443 - Pf1N1B4_1569 0.39 +0.4 1,805,443 - Pf1N1B4_1569 0.39 -0.2 1,805,443 - Pf1N1B4_1569 0.39 -1.6 1,805,443 - Pf1N1B4_1569 0.39 -0.2 1,805,443 - Pf1N1B4_1569 0.39 -0.6 1,805,443 - Pf1N1B4_1569 0.39 -0.6 1,805,443 - Pf1N1B4_1569 0.39 -0.2 1,805,499 + Pf1N1B4_1569 0.46 -0.6 1,805,499 + Pf1N1B4_1569 0.46 +0.4 1,805,499 + Pf1N1B4_1569 0.46 +0.5 1,805,499 + Pf1N1B4_1569 0.46 -2.1 1,805,499 + Pf1N1B4_1569 0.46 +1.9 1,805,499 + Pf1N1B4_1569 0.46 -1.8 1,805,500 - -1.6 1,805,500 - Pf1N1B4_1569 0.46 -0.1 1,805,500 - Pf1N1B4_1569 0.46 +0.2 1,805,500 - Pf1N1B4_1569 0.46 +0.9 1,805,500 - Pf1N1B4_1569 0.46 -1.0 1,805,500 - Pf1N1B4_1569 0.46 +0.8 1,805,500 - Pf1N1B4_1569 0.46 +3.1 1,805,500 - Pf1N1B4_1569 0.46 -0.2 1,805,520 + Pf1N1B4_1569 0.49 -0.7 1,805,520 + Pf1N1B4_1569 0.49 -1.2 1,805,520 + Pf1N1B4_1569 0.49 -1.3 1,805,520 + Pf1N1B4_1569 0.49 +0.6 1,805,521 - Pf1N1B4_1569 0.49 -0.2 1,805,604 + Pf1N1B4_1569 0.59 +1.4 1,805,604 + Pf1N1B4_1569 0.59 -0.2 1,805,604 + Pf1N1B4_1569 0.59 -2.8 1,805,604 + Pf1N1B4_1569 0.59 -2.3 1,805,604 + Pf1N1B4_1569 0.59 +0.8 1,805,604 + Pf1N1B4_1569 0.59 -1.2 1,805,604 + Pf1N1B4_1569 0.59 -0.9 1,805,605 - Pf1N1B4_1569 0.59 +0.4 1,805,605 - Pf1N1B4_1569 0.59 -1.2 1,805,605 - Pf1N1B4_1569 0.59 -0.6 1,805,605 - Pf1N1B4_1569 0.59 -0.2 1,805,605 - Pf1N1B4_1569 0.59 -1.0 1,805,605 - Pf1N1B4_1569 0.59 -0.6 1,805,605 - Pf1N1B4_1569 0.59 -1.6 1,805,605 - Pf1N1B4_1569 0.59 +0.9 1,805,605 - Pf1N1B4_1569 0.59 +0.4 1,805,706 + Pf1N1B4_1569 0.72 -0.1 1,805,706 + Pf1N1B4_1569 0.72 -0.1 1,805,706 + Pf1N1B4_1569 0.72 -0.5 1,805,706 + Pf1N1B4_1569 0.72 -0.5 1,805,706 + Pf1N1B4_1569 0.72 -0.0 1,805,706 + Pf1N1B4_1569 0.72 -2.0 1,805,707 - Pf1N1B4_1569 0.72 +0.2 1,805,727 + Pf1N1B4_1569 0.75 +1.6 1,805,727 + Pf1N1B4_1569 0.75 -0.1 1,805,727 + Pf1N1B4_1569 0.75 -2.0 1,805,727 + Pf1N1B4_1569 0.75 +0.0 1,805,727 + Pf1N1B4_1569 0.75 -0.6 1,805,728 - Pf1N1B4_1569 0.75 -0.4 1,805,728 - Pf1N1B4_1569 0.75 -1.1 1,805,728 - Pf1N1B4_1569 0.75 -0.6 1,805,728 - Pf1N1B4_1569 0.75 -0.5 1,805,728 - Pf1N1B4_1569 0.75 -2.2 1,805,728 - Pf1N1B4_1569 0.75 -2.0 1,806,081 + Pf1N1B4_1570 0.22 -1.5 1,806,081 + Pf1N1B4_1570 0.22 +1.2 1,806,082 - Pf1N1B4_1570 0.22 -1.7 1,806,082 - +0.3 1,806,154 - Pf1N1B4_1570 0.32 +1.3 1,806,452 + Pf1N1B4_1570 0.75 -1.0 1,806,452 + Pf1N1B4_1570 0.75 -1.7 1,806,453 - Pf1N1B4_1570 0.75 -2.2 1,806,453 - Pf1N1B4_1570 0.75 +0.3 1,806,453 - Pf1N1B4_1570 0.75 +1.3 1,806,453 - Pf1N1B4_1570 0.75 -1.5 1,806,453 - Pf1N1B4_1570 0.75 -3.2 1,806,453 - Pf1N1B4_1570 0.75 -0.7 1,806,645 - +0.3 1,806,717 + +0.3 1,806,775 + -2.5 1,806,784 - +0.3 1,806,784 - -0.7 1,806,818 + -1.2 1,806,828 + -0.1 1,806,828 + -0.2 1,806,828 + +0.9 1,806,828 + +0.9 1,806,828 + +0.8 1,806,829 - -2.2 1,806,844 + -1.2 1,806,844 + +0.3 1,806,845 - +0.3 1,806,854 + +0.2 1,806,854 + +1.7 1,806,854 + -0.7 1,806,854 + +1.7 1,806,855 - +0.8 1,806,885 + +0.1 1,806,885 + -1.2 1,806,885 + +0.1 1,806,885 + -0.7 1,806,886 - -1.8 1,806,886 - -3.0 1,806,886 - -0.5 1,806,886 - +0.8 1,807,007 - +0.8 1,807,058 + Pf1N1B4_1571 0.11 +1.9 1,807,059 - Pf1N1B4_1571 0.11 -0.4 1,807,206 - Pf1N1B4_1571 0.19 +0.3 1,807,225 + Pf1N1B4_1571 0.20 +0.6 1,807,226 - Pf1N1B4_1571 0.20 -0.1 1,807,226 - Pf1N1B4_1571 0.20 +0.3 1,807,415 + Pf1N1B4_1571 0.29 -0.4 1,807,415 + Pf1N1B4_1571 0.29 +0.3 1,807,415 + Pf1N1B4_1571 0.29 -0.7 1,807,415 + Pf1N1B4_1571 0.29 -2.8 1,807,415 + Pf1N1B4_1571 0.29 -1.5 1,807,415 + Pf1N1B4_1571 0.29 -0.7 1,807,416 - -0.2 1,807,416 - Pf1N1B4_1571 0.29 +0.6 1,807,416 - Pf1N1B4_1571 0.29 -0.7 1,807,416 - Pf1N1B4_1571 0.29 -2.0 1,807,416 - Pf1N1B4_1571 0.29 -1.2 1,807,416 - +0.3 1,807,416 - Pf1N1B4_1571 0.29 +0.9 1,807,416 - Pf1N1B4_1571 0.29 +1.1 1,807,416 - Pf1N1B4_1571 0.29 -0.2 1,807,416 - Pf1N1B4_1571 0.29 -0.7 1,807,418 - Pf1N1B4_1571 0.30 -2.0
Or see this region's nucleotide sequence