Experiment: Recovery broth with Nisin from Lactococcus lactis 7e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS15965 and BBR_RS15970 are separated by 203 nucleotides BBR_RS15970 and BBR_RS15975 overlap by 4 nucleotides BBR_RS15975 and BBR_RS15980 are separated by 4 nucleotides
BBR_RS15965: BBR_RS15965 - transporter, drug/metabolite exporter family, at 1,338,931 to 1,339,806
_RS15965
BBR_RS15970: BBR_RS15970 - hypothetical protein, at 1,340,010 to 1,340,633
_RS15970
BBR_RS15975: BBR_RS15975 - hypothetical protein, at 1,340,630 to 1,341,358
_RS15975
BBR_RS15980: BBR_RS15980 - Lantibiotic transport ATP-binding protein, at 1,341,363 to 1,342,019
_RS15980
Position (kb)
1340
1341 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1339.027 kb on - strand, within BBR_RS15965 at 1339.073 kb on + strand, within BBR_RS15965 at 1339.073 kb on + strand, within BBR_RS15965 at 1339.148 kb on + strand, within BBR_RS15965 at 1339.234 kb on - strand, within BBR_RS15965 at 1339.239 kb on - strand, within BBR_RS15965 at 1339.247 kb on - strand, within BBR_RS15965 at 1339.247 kb on - strand, within BBR_RS15965 at 1339.306 kb on + strand, within BBR_RS15965 at 1339.331 kb on + strand, within BBR_RS15965 at 1339.331 kb on + strand, within BBR_RS15965 at 1339.332 kb on - strand, within BBR_RS15965 at 1339.332 kb on - strand, within BBR_RS15965 at 1339.347 kb on + strand, within BBR_RS15965 at 1339.400 kb on + strand, within BBR_RS15965 at 1339.426 kb on - strand, within BBR_RS15965 at 1339.438 kb on - strand, within BBR_RS15965 at 1339.451 kb on - strand, within BBR_RS15965 at 1339.482 kb on + strand, within BBR_RS15965 at 1339.490 kb on - strand, within BBR_RS15965 at 1339.684 kb on - strand, within BBR_RS15965 at 1339.717 kb on + strand, within BBR_RS15965 at 1339.748 kb on - strand at 1339.768 kb on - strand at 1339.772 kb on - strand at 1339.772 kb on - strand at 1339.779 kb on - strand at 1339.843 kb on - strand at 1339.848 kb on - strand at 1339.894 kb on - strand at 1339.901 kb on - strand at 1339.978 kb on + strand at 1340.039 kb on - strand at 1340.103 kb on + strand, within BBR_RS15970 at 1340.103 kb on + strand, within BBR_RS15970 at 1340.103 kb on + strand, within BBR_RS15970 at 1340.229 kb on - strand, within BBR_RS15970 at 1340.291 kb on - strand, within BBR_RS15970 at 1340.326 kb on - strand, within BBR_RS15970 at 1340.364 kb on + strand, within BBR_RS15970 at 1340.388 kb on + strand, within BBR_RS15970 at 1340.453 kb on - strand, within BBR_RS15970 at 1340.465 kb on + strand, within BBR_RS15970 at 1340.490 kb on - strand, within BBR_RS15970 at 1340.590 kb on - strand at 1340.726 kb on - strand, within BBR_RS15975 at 1340.867 kb on + strand, within BBR_RS15975 at 1340.871 kb on - strand, within BBR_RS15975 at 1340.916 kb on + strand, within BBR_RS15975 at 1340.937 kb on + strand, within BBR_RS15975 at 1340.939 kb on + strand, within BBR_RS15975 at 1340.947 kb on - strand, within BBR_RS15975 at 1340.957 kb on + strand, within BBR_RS15975 at 1340.965 kb on - strand, within BBR_RS15975 at 1340.996 kb on + strand, within BBR_RS15975 at 1341.008 kb on + strand, within BBR_RS15975 at 1341.023 kb on + strand, within BBR_RS15975 at 1341.047 kb on + strand, within BBR_RS15975 at 1341.055 kb on - strand, within BBR_RS15975 at 1341.080 kb on - strand, within BBR_RS15975 at 1341.099 kb on + strand, within BBR_RS15975 at 1341.128 kb on - strand, within BBR_RS15975 at 1341.174 kb on - strand, within BBR_RS15975 at 1341.179 kb on - strand, within BBR_RS15975 at 1341.190 kb on - strand, within BBR_RS15975 at 1341.265 kb on + strand, within BBR_RS15975 at 1341.279 kb on + strand, within BBR_RS15975 at 1341.287 kb on - strand at 1341.351 kb on - strand at 1341.361 kb on + strand at 1341.396 kb on - strand at 1341.402 kb on - strand at 1341.421 kb on + strand at 1341.447 kb on - strand, within BBR_RS15980 at 1341.468 kb on - strand, within BBR_RS15980 at 1341.468 kb on - strand, within BBR_RS15980 at 1341.468 kb on - strand, within BBR_RS15980 at 1341.468 kb on - strand, within BBR_RS15980 at 1341.566 kb on - strand, within BBR_RS15980 at 1341.566 kb on - strand, within BBR_RS15980 at 1341.613 kb on + strand, within BBR_RS15980
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nisin from Lactococcus lactis 7e-04 mM remove 1,339,027 - BBR_RS15965 0.11 -0.2 1,339,073 + BBR_RS15965 0.16 -0.3 1,339,073 + BBR_RS15965 0.16 -1.8 1,339,148 + BBR_RS15965 0.25 -0.1 1,339,234 - BBR_RS15965 0.35 +1.0 1,339,239 - BBR_RS15965 0.35 +0.7 1,339,247 - BBR_RS15965 0.36 -2.1 1,339,247 - BBR_RS15965 0.36 +0.1 1,339,306 + BBR_RS15965 0.43 +0.8 1,339,331 + BBR_RS15965 0.46 +0.8 1,339,331 + BBR_RS15965 0.46 +1.6 1,339,332 - BBR_RS15965 0.46 +0.8 1,339,332 - BBR_RS15965 0.46 -0.1 1,339,347 + BBR_RS15965 0.47 +0.2 1,339,400 + BBR_RS15965 0.54 +0.6 1,339,426 - BBR_RS15965 0.57 -0.3 1,339,438 - BBR_RS15965 0.58 +0.2 1,339,451 - BBR_RS15965 0.59 +1.3 1,339,482 + BBR_RS15965 0.63 -1.4 1,339,490 - BBR_RS15965 0.64 -0.7 1,339,684 - BBR_RS15965 0.86 +0.0 1,339,717 + BBR_RS15965 0.90 +1.5 1,339,748 - +0.3 1,339,768 - +0.8 1,339,772 - -0.1 1,339,772 - -0.4 1,339,779 - +2.1 1,339,843 - +0.2 1,339,848 - -0.5 1,339,894 - -0.9 1,339,901 - +0.7 1,339,978 + -1.4 1,340,039 - +0.4 1,340,103 + BBR_RS15970 0.15 -3.5 1,340,103 + BBR_RS15970 0.15 -3.7 1,340,103 + BBR_RS15970 0.15 -2.9 1,340,229 - BBR_RS15970 0.35 -1.5 1,340,291 - BBR_RS15970 0.45 -2.2 1,340,326 - BBR_RS15970 0.51 -2.8 1,340,364 + BBR_RS15970 0.57 -3.3 1,340,388 + BBR_RS15970 0.61 -3.8 1,340,453 - BBR_RS15970 0.71 -2.3 1,340,465 + BBR_RS15970 0.73 -0.8 1,340,490 - BBR_RS15970 0.77 -2.7 1,340,590 - -3.2 1,340,726 - BBR_RS15975 0.13 -4.8 1,340,867 + BBR_RS15975 0.33 -1.4 1,340,871 - BBR_RS15975 0.33 -2.8 1,340,916 + BBR_RS15975 0.39 -3.3 1,340,937 + BBR_RS15975 0.42 -0.0 1,340,939 + BBR_RS15975 0.42 -2.5 1,340,947 - BBR_RS15975 0.43 -2.7 1,340,957 + BBR_RS15975 0.45 -4.0 1,340,965 - BBR_RS15975 0.46 -4.0 1,340,996 + BBR_RS15975 0.50 -2.8 1,341,008 + BBR_RS15975 0.52 -1.8 1,341,023 + BBR_RS15975 0.54 -3.5 1,341,047 + BBR_RS15975 0.57 -2.7 1,341,055 - BBR_RS15975 0.58 -2.4 1,341,080 - BBR_RS15975 0.62 -3.4 1,341,099 + BBR_RS15975 0.64 -3.7 1,341,128 - BBR_RS15975 0.68 -3.0 1,341,174 - BBR_RS15975 0.75 -4.4 1,341,179 - BBR_RS15975 0.75 -4.3 1,341,190 - BBR_RS15975 0.77 -3.7 1,341,265 + BBR_RS15975 0.87 +0.8 1,341,279 + BBR_RS15975 0.89 -2.4 1,341,287 - -1.1 1,341,351 - -3.5 1,341,361 + -1.9 1,341,396 - -3.5 1,341,402 - -3.1 1,341,421 + -3.4 1,341,447 - BBR_RS15980 0.13 -1.5 1,341,468 - BBR_RS15980 0.16 -5.0 1,341,468 - BBR_RS15980 0.16 -2.4 1,341,468 - BBR_RS15980 0.16 -4.0 1,341,468 - BBR_RS15980 0.16 -2.6 1,341,566 - BBR_RS15980 0.31 -2.5 1,341,566 - BBR_RS15980 0.31 -4.3 1,341,613 + BBR_RS15980 0.38 -2.9
Or see this region's nucleotide sequence