Experiment: Recovery broth with Nisin from Lactococcus lactis 7e-04 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14140 and BBR_RS14145 are separated by 33 nucleotides BBR_RS14145 and BBR_RS14150 are separated by 126 nucleotides BBR_RS14150 and BBR_RS14155 are separated by 131 nucleotides BBR_RS14155 and BBR_RS14160 are separated by 60 nucleotides BBR_RS14160 and BBR_RS14165 are separated by 97 nucleotides
BBR_RS14140: BBR_RS14140 - Ppx/GppA family phosphatase, at 949,005 to 950,006
_RS14140
BBR_RS14145: BBR_RS14145 - tRNA-Leu, at 950,040 to 950,119
_RS14145
BBR_RS14150: BBR_RS14150 - hypothetical protein, at 950,246 to 950,815
_RS14150
BBR_RS14155: BBR_RS14155 - L-serine ammonia-lyase, at 950,947 to 952,404
_RS14155
BBR_RS14160: BBR_RS14160 - peptidylprolyl isomerase, at 952,465 to 952,872
_RS14160
BBR_RS14165: BBR_RS14165 - transcription elongation factor GreA, at 952,970 to 953,449
_RS14165
Position (kb)
950
951
952
953 Strain fitness (log2 ratio)
-2
-1
0
1 at 949.961 kb on + strand at 950.007 kb on - strand at 950.009 kb on - strand at 950.015 kb on + strand at 950.192 kb on + strand at 950.202 kb on + strand at 950.202 kb on + strand at 950.210 kb on - strand at 950.242 kb on + strand at 950.296 kb on - strand at 950.361 kb on + strand, within BBR_RS14150 at 950.428 kb on - strand, within BBR_RS14150 at 950.505 kb on + strand, within BBR_RS14150 at 950.518 kb on + strand, within BBR_RS14150 at 950.519 kb on + strand, within BBR_RS14150 at 950.545 kb on - strand, within BBR_RS14150 at 950.610 kb on + strand, within BBR_RS14150 at 950.652 kb on + strand, within BBR_RS14150 at 950.722 kb on + strand, within BBR_RS14150 at 950.723 kb on + strand, within BBR_RS14150 at 950.730 kb on + strand, within BBR_RS14150 at 950.738 kb on - strand, within BBR_RS14150 at 950.754 kb on - strand, within BBR_RS14150 at 950.878 kb on + strand at 950.893 kb on + strand at 950.944 kb on + strand at 950.946 kb on + strand at 950.952 kb on - strand at 950.952 kb on - strand at 951.010 kb on - strand at 951.010 kb on - strand at 951.093 kb on + strand, within BBR_RS14155 at 951.150 kb on - strand, within BBR_RS14155 at 951.166 kb on + strand, within BBR_RS14155 at 951.174 kb on - strand, within BBR_RS14155 at 951.176 kb on + strand, within BBR_RS14155 at 951.177 kb on + strand, within BBR_RS14155 at 951.222 kb on + strand, within BBR_RS14155 at 951.246 kb on + strand, within BBR_RS14155 at 951.254 kb on - strand, within BBR_RS14155 at 951.308 kb on - strand, within BBR_RS14155 at 951.357 kb on - strand, within BBR_RS14155 at 951.404 kb on + strand, within BBR_RS14155 at 951.451 kb on - strand, within BBR_RS14155 at 951.462 kb on + strand, within BBR_RS14155 at 951.462 kb on + strand, within BBR_RS14155 at 951.481 kb on - strand, within BBR_RS14155 at 951.510 kb on + strand, within BBR_RS14155 at 951.518 kb on - strand, within BBR_RS14155 at 951.621 kb on + strand, within BBR_RS14155 at 951.621 kb on + strand, within BBR_RS14155 at 951.659 kb on - strand, within BBR_RS14155 at 951.678 kb on - strand, within BBR_RS14155 at 951.697 kb on + strand, within BBR_RS14155 at 951.737 kb on + strand, within BBR_RS14155 at 951.751 kb on + strand, within BBR_RS14155 at 951.755 kb on - strand, within BBR_RS14155 at 951.796 kb on - strand, within BBR_RS14155 at 951.827 kb on + strand, within BBR_RS14155 at 951.827 kb on + strand, within BBR_RS14155 at 951.868 kb on + strand, within BBR_RS14155 at 951.872 kb on - strand, within BBR_RS14155 at 951.912 kb on + strand, within BBR_RS14155 at 951.915 kb on + strand, within BBR_RS14155 at 951.992 kb on + strand, within BBR_RS14155 at 951.992 kb on + strand, within BBR_RS14155 at 952.022 kb on - strand, within BBR_RS14155 at 952.065 kb on + strand, within BBR_RS14155 at 952.091 kb on + strand, within BBR_RS14155 at 952.129 kb on - strand, within BBR_RS14155 at 952.160 kb on + strand, within BBR_RS14155 at 952.174 kb on - strand, within BBR_RS14155 at 952.178 kb on + strand, within BBR_RS14155 at 952.178 kb on + strand, within BBR_RS14155 at 952.191 kb on + strand, within BBR_RS14155 at 952.260 kb on + strand at 952.260 kb on + strand at 952.269 kb on - strand at 952.308 kb on + strand at 952.352 kb on + strand at 952.537 kb on + strand, within BBR_RS14160 at 952.537 kb on + strand, within BBR_RS14160 at 952.566 kb on + strand, within BBR_RS14160 at 952.572 kb on + strand, within BBR_RS14160 at 952.572 kb on + strand, within BBR_RS14160 at 952.615 kb on + strand, within BBR_RS14160 at 952.640 kb on + strand, within BBR_RS14160 at 952.671 kb on + strand, within BBR_RS14160
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Nisin from Lactococcus lactis 7e-04 mM remove 949,961 + +0.1 950,007 - -0.1 950,009 - +0.5 950,015 + -0.2 950,192 + -0.1 950,202 + +0.4 950,202 + +0.4 950,210 - -0.4 950,242 + +0.9 950,296 - +0.4 950,361 + BBR_RS14150 0.20 +0.2 950,428 - BBR_RS14150 0.32 -0.5 950,505 + BBR_RS14150 0.45 -0.2 950,518 + BBR_RS14150 0.48 +0.6 950,519 + BBR_RS14150 0.48 -0.0 950,545 - BBR_RS14150 0.52 +0.7 950,610 + BBR_RS14150 0.64 -1.4 950,652 + BBR_RS14150 0.71 +0.5 950,722 + BBR_RS14150 0.84 +0.1 950,723 + BBR_RS14150 0.84 +0.3 950,730 + BBR_RS14150 0.85 -0.7 950,738 - BBR_RS14150 0.86 +0.3 950,754 - BBR_RS14150 0.89 +0.5 950,878 + -0.2 950,893 + +0.6 950,944 + +0.2 950,946 + +0.3 950,952 - +0.8 950,952 - +0.3 951,010 - +1.2 951,010 - +0.9 951,093 + BBR_RS14155 0.10 -0.0 951,150 - BBR_RS14155 0.14 -0.3 951,166 + BBR_RS14155 0.15 +0.1 951,174 - BBR_RS14155 0.16 -0.1 951,176 + BBR_RS14155 0.16 +1.1 951,177 + BBR_RS14155 0.16 -0.1 951,222 + BBR_RS14155 0.19 +1.2 951,246 + BBR_RS14155 0.21 -0.7 951,254 - BBR_RS14155 0.21 -0.8 951,308 - BBR_RS14155 0.25 -0.6 951,357 - BBR_RS14155 0.28 +0.2 951,404 + BBR_RS14155 0.31 -0.1 951,451 - BBR_RS14155 0.35 +1.4 951,462 + BBR_RS14155 0.35 -0.1 951,462 + BBR_RS14155 0.35 +0.3 951,481 - BBR_RS14155 0.37 +0.8 951,510 + BBR_RS14155 0.39 -0.4 951,518 - BBR_RS14155 0.39 -0.9 951,621 + BBR_RS14155 0.46 -0.8 951,621 + BBR_RS14155 0.46 +0.7 951,659 - BBR_RS14155 0.49 +0.6 951,678 - BBR_RS14155 0.50 +0.9 951,697 + BBR_RS14155 0.51 +0.8 951,737 + BBR_RS14155 0.54 +0.2 951,751 + BBR_RS14155 0.55 -0.3 951,755 - BBR_RS14155 0.55 +0.5 951,796 - BBR_RS14155 0.58 -1.8 951,827 + BBR_RS14155 0.60 +0.5 951,827 + BBR_RS14155 0.60 +1.2 951,868 + BBR_RS14155 0.63 -0.1 951,872 - BBR_RS14155 0.63 -0.7 951,912 + BBR_RS14155 0.66 -0.3 951,915 + BBR_RS14155 0.66 -0.9 951,992 + BBR_RS14155 0.72 +0.3 951,992 + BBR_RS14155 0.72 +0.0 952,022 - BBR_RS14155 0.74 -0.3 952,065 + BBR_RS14155 0.77 -2.7 952,091 + BBR_RS14155 0.78 +0.5 952,129 - BBR_RS14155 0.81 -0.2 952,160 + BBR_RS14155 0.83 +0.5 952,174 - BBR_RS14155 0.84 -1.4 952,178 + BBR_RS14155 0.84 -0.0 952,178 + BBR_RS14155 0.84 +0.1 952,191 + BBR_RS14155 0.85 -0.1 952,260 + -1.2 952,260 + +1.4 952,269 - -0.4 952,308 + +0.3 952,352 + -0.6 952,537 + BBR_RS14160 0.18 +0.2 952,537 + BBR_RS14160 0.18 -0.2 952,566 + BBR_RS14160 0.25 -0.2 952,572 + BBR_RS14160 0.26 +0.5 952,572 + BBR_RS14160 0.26 +1.4 952,615 + BBR_RS14160 0.37 -0.2 952,640 + BBR_RS14160 0.43 +0.4 952,671 + BBR_RS14160 0.50 +0.2
Or see this region's nucleotide sequence