Experiment: Defined MoLS4
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and DvMF_2216 are separated by 13 nucleotides DvMF_2216 and DvMF_2217 are separated by 179 nucleotides DvMF_2217 and DvMF_2218 are separated by 655 nucleotides
DvMF_2215: fliP - flagellar biosynthesis protein FliP (RefSeq), at 2,767,902 to 2,768,783
fliP
DvMF_2216: DvMF_2216 - flagellar biosynthetic protein FliQ (RefSeq), at 2,768,797 to 2,769,066
_2216
DvMF_2217: DvMF_2217 - tRNA/rRNA methyltransferase (SpoU) (RefSeq), at 2,769,246 to 2,770,280
_2217
DvMF_2218: DvMF_2218 - Lytic transglycosylase catalytic (RefSeq), at 2,770,936 to 2,772,792
_2218
Position (kb)
2769
2770
2771 Strain fitness (log2 ratio)
-1
0
1 at 2768.299 kb on - strand, within fliP at 2768.315 kb on - strand, within fliP at 2768.466 kb on - strand, within fliP at 2768.575 kb on + strand, within fliP at 2768.892 kb on - strand, within DvMF_2216 at 2769.297 kb on - strand at 2769.309 kb on - strand at 2769.353 kb on - strand, within DvMF_2217 at 2769.617 kb on - strand, within DvMF_2217 at 2770.026 kb on - strand, within DvMF_2217 at 2770.026 kb on - strand, within DvMF_2217 at 2770.223 kb on + strand at 2770.375 kb on - strand at 2770.797 kb on - strand at 2770.958 kb on + strand at 2770.958 kb on + strand at 2771.081 kb on - strand at 2771.116 kb on - strand at 2771.144 kb on - strand, within DvMF_2218 at 2771.178 kb on + strand, within DvMF_2218
Per-strain Table
Position Strand Gene LocusTag Fraction Defined MoLS4 remove 2,768,299 - fliP DvMF_2215 0.45 +0.8 2,768,315 - fliP DvMF_2215 0.47 +0.9 2,768,466 - fliP DvMF_2215 0.64 +0.7 2,768,575 + fliP DvMF_2215 0.76 +0.7 2,768,892 - DvMF_2216 0.35 +0.9 2,769,297 - -0.6 2,769,309 - -0.7 2,769,353 - DvMF_2217 0.10 -0.1 2,769,617 - DvMF_2217 0.36 -0.2 2,770,026 - DvMF_2217 0.75 -0.8 2,770,026 - DvMF_2217 0.75 -0.4 2,770,223 + -0.0 2,770,375 - -0.9 2,770,797 - -0.3 2,770,958 + -0.2 2,770,958 + -0.4 2,771,081 - -0.7 2,771,116 - +0.4 2,771,144 - DvMF_2218 0.11 -0.4 2,771,178 + DvMF_2218 0.13 -0.5
Or see this region's nucleotide sequence